Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum
Identifieur interne : 000835 ( Istex/Corpus ); précédent : 000834; suivant : 000836Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum
Auteurs : John F. Lechner ; Aage Haugen ; Irene A. Mcclendon ; Abulkalam M. ShamsuddinSource :
- Differentiation [ 0301-4681 ] ; 1983.
Abstract
Recently we reported a low calcium (110 M) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 2) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 2). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8 serum. Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2 (up to 1,000 M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 M) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.
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DOI: 10.1111/j.1432-0436.1984.tb01361.x
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum</title><author><name sortKey="Lechner, John F" sort="Lechner, John F" uniqKey="Lechner J" first="John F." last="Lechner">John F. Lechner</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author><author><name sortKey="Haugen, Aage" sort="Haugen, Aage" uniqKey="Haugen A" first="Aage" last="Haugen">Aage Haugen</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author><author><name sortKey="Mcclendon, Irene A" sort="Mcclendon, Irene A" uniqKey="Mcclendon I" first="Irene A." last="Mcclendon">Irene A. Mcclendon</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author><author><name sortKey="Shamsuddin, Abulkalam M" sort="Shamsuddin, Abulkalam M" uniqKey="Shamsuddin A" first="Abulkalam M." last="Shamsuddin">Abulkalam M. Shamsuddin</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0AACB1F87C5856C15AAD53899BFD8907734058EA</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1111/j.1432-0436.1984.tb01361.x</idno><idno type="url">https://api.istex.fr/document/0AACB1F87C5856C15AAD53899BFD8907734058EA/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000835</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum</title><author><name sortKey="Lechner, John F" sort="Lechner, John F" uniqKey="Lechner J" first="John F." last="Lechner">John F. Lechner</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author><author><name sortKey="Haugen, Aage" sort="Haugen, Aage" uniqKey="Haugen A" first="Aage" last="Haugen">Aage Haugen</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author><author><name sortKey="Mcclendon, Irene A" sort="Mcclendon, Irene A" uniqKey="Mcclendon I" first="Irene A." last="Mcclendon">Irene A. Mcclendon</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author><author><name sortKey="Shamsuddin, Abulkalam M" sort="Shamsuddin, Abulkalam M" uniqKey="Shamsuddin A" first="Abulkalam M." last="Shamsuddin">Abulkalam M. Shamsuddin</name><affiliation><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Differentiation</title><title level="j" type="abbrev">DIFF</title><idno type="ISSN">0301-4681</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983">1983</date><biblScope unit="volume">25</biblScope><biblScope unit="issue">1–3</biblScope><biblScope unit="page" from="229">229</biblScope><biblScope unit="page" to="237">237</biblScope></imprint><idno type="ISSN">0301-4681</idno></series><idno type="istex">0AACB1F87C5856C15AAD53899BFD8907734058EA</idno><idno type="DOI">10.1111/j.1432-0436.1984.tb01361.x</idno><idno type="PII">S0301-4681(11)61612-5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0301-4681</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recently we reported a low calcium (110 M) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 2) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 2). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8 serum. Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2 (up to 1,000 M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 M) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>John F. Lechner</name><affiliations><json:string>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</json:string></affiliations></json:item><json:item><name>Aage Haugen</name><affiliations><json:string>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</json:string></affiliations></json:item><json:item><name>Irene A. McClendon</name><affiliations><json:string>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</json:string></affiliations></json:item><json:item><name>Abulkalam M. Shamsuddin</name><affiliations><json:string>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><abstract>Recently we reported a low calcium (110 M) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 2) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 2). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8 serum. Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2 (up to 1,000 M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 M) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.</abstract><qualityIndicators><score>9.723</score><pdfVersion>1.7</pdfVersion><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1724</abstractCharCount><pdfWordCount>4723</pdfWordCount><pdfCharCount>29208</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>270</abstractWordCount></qualityIndicators><title>Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of 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level="a">Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1984</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum</title><author><persName><forename type="first">John F.</forename><surname>Lechner</surname></persName><note type="biography">National Institutes of Health, National Cancer Institute, Building 37, Room 2C27, Bethesda, Maryland 20205, USA</note><affiliation>National Institutes of Health, National Cancer Institute, Building 37, Room 2C27, Bethesda, Maryland 20205, USA</affiliation><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation></author><author><persName><forename type="first">Aage</forename><surname>Haugen</surname></persName><note type="biography">Current address: National Institute of Public Health, Department of Toxicology, Oslo, Norway</note><affiliation>Current address: National Institute of Public Health, Department of Toxicology, Oslo, Norway</affiliation><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation></author><author><persName><forename type="first">Irene A.</forename><surname>McClendon</surname></persName><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation></author><author><persName><forename type="first">Abulkalam M.</forename><surname>Shamsuddin</surname></persName><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation></author></analytic><monogr><title level="j">Differentiation</title><title level="j" type="abbrev">DIFF</title><idno type="pISSN">0301-4681</idno><idno type="PII">S0301-4681(11)X6128-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983"></date><biblScope unit="volume">25</biblScope><biblScope unit="issue">1–3</biblScope><biblScope unit="page" from="229">229</biblScope><biblScope unit="page" to="237">237</biblScope></imprint></monogr><idno type="istex">0AACB1F87C5856C15AAD53899BFD8907734058EA</idno><idno type="DOI">10.1111/j.1432-0436.1984.tb01361.x</idno><idno type="PII">S0301-4681(11)61612-5</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1984</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Recently we reported a low calcium (110 M) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 2) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 2). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8 serum. Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2 (up to 1,000 M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 M) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.</p></abstract></profileDesc><revisionDesc><change when="1983-08">Registration</change><change when="1983">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.1.0//EN//XML" URI="art510.dtd" name="istex:docType"></istex:docType><istex:document><article docsubtype="fla"> <item-info> <jid>DIFF</jid> <aid>61612</aid> <ce:pii>S0301-4681(11)61612-5</ce:pii> <ce:doi>10.1111/j.1432-0436.1984.tb01361.x</ce:doi> <ce:copyright type="society" year="1984">International Society of Differentiation</ce:copyright> </item-info> <head> <ce:title>Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum</ce:title> <ce:author-group> <ce:author> <ce:given-name>John F.</ce:given-name> <ce:surname>Lechner</ce:surname><ce:cross-ref refid="fn1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="aff1"><ce:sup>a</ce:sup></ce:cross-ref> </ce:author> <ce:author> <ce:given-name>Aage</ce:given-name> <ce:surname>Haugen</ce:surname><ce:cross-ref refid="fn2"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="aff1"><ce:sup>a</ce:sup></ce:cross-ref> </ce:author> <ce:author> <ce:given-name>Irene A.</ce:given-name> <ce:surname>McClendon</ce:surname><ce:cross-ref refid="aff1"><ce:sup>a</ce:sup></ce:cross-ref> </ce:author> <ce:author> <ce:given-name>Abulkalam M.</ce:given-name> <ce:surname>Shamsuddin</ce:surname><ce:cross-ref refid="aff1"><ce:sup>a</ce:sup></ce:cross-ref> </ce:author> <ce:affiliation id="aff1"> <ce:label>a</ce:label> <ce:textfn>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</ce:textfn> </ce:affiliation> <ce:footnote id="fn1"> <ce:label>1</ce:label> <ce:note-para>National Institutes of Health, National Cancer Institute, Building 37, Room 2C27, Bethesda, Maryland 20205, USA</ce:note-para> </ce:footnote> <ce:footnote id="fn2"> <ce:label>2</ce:label> <ce:note-para><ce:italic>Current address</ce:italic>: National Institute of Public Health, Department of Toxicology, Oslo, Norway</ce:note-para> </ce:footnote> </ce:author-group> <ce:date-received month="2" year="1983"></ce:date-received> <ce:date-accepted month="8" year="1983"></ce:date-accepted> <ce:abstract> <ce:section-title>Abstract</ce:section-title> <ce:abstract-sec> <ce:simple-para id="spara10">Recently we reported a low calcium (110 μM) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 μ<ce:sup>2</ce:sup>) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2%) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 μ<ce:sup>2</ce:sup>). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8% serum.</ce:simple-para> <ce:simple-para id="spara20">Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca<ce:sup>2+</ce:sup> (up to 1,000 μM) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 μM) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.</ce:simple-para> </ce:abstract-sec> </ce:abstract> </head> <tail> <ce:bibliography id="bibl10"> <ce:section-title>References</ce:section-title> <ce:bibliography-sec id="cebibsec10"> <ce:bib-reference id="bib1"> <ce:label>1</ce:label> <sb:reference> <sb:contribution> <sb:authors> <sb:author> <ce:given-name>PA</ce:given-name> <ce:surname>Buckley</ce:surname> </sb:author> <sb:author> <ce:given-name>IR</ce:given-name> <ce:surname>Konigsberg</ce:surname> </sb:author> </sb:authors> <sb:title> <sb:maintitle>Myogenic fusion and the duration of the post-mitotic gap (G<ce:inf>1</ce:inf>)</sb:maintitle> </sb:title> </sb:contribution> <sb:host> <sb:issue> <sb:series> <sb:title> <sb:maintitle>Dev Biol</sb:maintitle> </sb:title> <sb:volume-nr>37</sb:volume-nr> </sb:series> <sb:date>1974</sb:date> </sb:issue> <sb:pages> <sb:first-page>193</sb:first-page> <sb:last-page>212</sb:last-page> </sb:pages> </sb:host> </sb:reference> </ce:bib-reference> <ce:bib-reference id="bib2"> <ce:label>2</ce:label> <sb:reference> <sb:contribution> <sb:authors> <sb:author> <ce:given-name>KAO</ce:given-name> <ce:surname>Ellem</ce:surname> </sb:author> <sb:author> <ce:given-name>JF</ce:given-name> <ce:surname>Gierthy</ce:surname> </sb:author> </sb:authors> <sb:title> <sb:maintitle>Mechanism of regulation of fibroblastic cell replication. <ce:italic>IV. 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small amounts of serum</title></titleInfo><name type="personal"><namePart type="given">John F.</namePart><namePart type="family">Lechner</namePart><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation><description>National Institutes of Health, National Cancer Institute, Building 37, Room 2C27, Bethesda, Maryland 20205, USA</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Aage</namePart><namePart type="family">Haugen</namePart><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation><description>Current address: National Institute of Public Health, Department of Toxicology, Oslo, Norway</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Irene A.</namePart><namePart type="family">McClendon</namePart><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Abulkalam M.</namePart><namePart type="family">Shamsuddin</namePart><affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1983</dateIssued><dateValid encoding="w3cdtf">1983-08</dateValid><copyrightDate encoding="w3cdtf">1984</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Recently we reported a low calcium (110 M) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 2) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 2). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8 serum. Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2 (up to 1,000 M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 M) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.</abstract><relatedItem type="host"><titleInfo><title>Differentiation</title></titleInfo><titleInfo type="abbreviated"><title>DIFF</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">198402</dateIssued></originInfo><identifier type="ISSN">0301-4681</identifier><identifier type="PII">S0301-4681(11)X6128-5</identifier><part><date>198402</date><detail type="volume"><number>25</number><caption>vol.</caption></detail><detail type="issue"><number>1–3</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>264</end></extent><extent unit="pages"><start>229</start><end>237</end></extent></part></relatedItem><identifier type="istex">0AACB1F87C5856C15AAD53899BFD8907734058EA</identifier><identifier type="DOI">10.1111/j.1432-0436.1984.tb01361.x</identifier><identifier type="PII">S0301-4681(11)61612-5</identifier><accessCondition type="use and reproduction" contentType="">© 1984International Society of Differentiation</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>International Society of Differentiation, ©1984</recordOrigin></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/0AACB1F87C5856C15AAD53899BFD8907734058EA/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">CELL BIOLOGY</classCode><classCode scheme="WOS">DEVELOPMENTAL BIOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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