Endothelial cell presentation of antigen to human T cells
Identifieur interne : 002684 ( Istex/Corpus ); précédent : 002683; suivant : 002685Endothelial cell presentation of antigen to human T cells
Auteurs : Denis R. Burger ; Dennis Ford ; R. Mark Vetto ; Anne Hamblin ; Andrew Goldstein ; Michael Hubbard ; D. C. DumondeSource :
- Human Immunology [ 0198-8859 ] ; 1981.
Abstract
Activation of human T cells requires presentation of antigen by Ia (HLA-DR in man) bearing cells of the mononuclear phagocytic series (macrophages, MØ), and more recently Langerbans cells, dendritic cells, and vascular endothelial cells. Since T cells must cross endothelial barriers to enter extavasulcar tissues during immune reactions, we investigated the role of endothelial cells in antigen presentation. Endothelial cells were cultured from human umbilical veins and identified by classic morphology and specific markers (factor VIII related antigen, and so on). Antigen-pulsed endothelial cells were used to present antigen to MØ-depleted human T cells; activation was assessed by 3H-thymidine uptake. The HLA-DR compatible endothelial cells were as effective as MØ-in reconstituting MØ-depleted T-cell responses. The endothelial cell reconstituted responses were antigen specific, HLA-DR restricted, and blocked by monoclonal antibodies to HLA-DR framework structures. Moreover, the T-cell responses were clonal with respect to HLA-DR. A monoclonal antibody completely eliminated MØ reconstitution of the MØ-depleted response without diminution of endothelial cell reconstitution of the same response. Fibroblasts and smooth muscle cells cultured from the same umbilical veins could not reconstitute the MØ-depleted T-cell response. These data indicate that endothelial cells play an important and distinctive role in lymphocyte triggering.
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DOI: 10.1016/0198-8859(81)90019-7
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Endothelial cell presentation of antigen to human T cells</title><author><name sortKey="Burger, Denis R" sort="Burger, Denis R" uniqKey="Burger D" first="Denis R." last="Burger">Denis R. Burger</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Ford, Dennis" sort="Ford, Dennis" uniqKey="Ford D" first="Dennis" last="Ford">Dennis Ford</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Mark Vetto, R" sort="Mark Vetto, R" uniqKey="Mark Vetto R" first="R." last="Mark Vetto">R. Mark Vetto</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Hamblin, Anne" sort="Hamblin, Anne" uniqKey="Hamblin A" first="Anne" last="Hamblin">Anne Hamblin</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Goldstein, Andrew" sort="Goldstein, Andrew" uniqKey="Goldstein A" first="Andrew" last="Goldstein">Andrew Goldstein</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Hubbard, Michael" sort="Hubbard, Michael" uniqKey="Hubbard M" first="Michael" last="Hubbard">Michael Hubbard</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Dumonde, D C" sort="Dumonde, D C" uniqKey="Dumonde D" first="D. C." last="Dumonde">D. C. Dumonde</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1AE2024806226D4EB99DE8392DA35B4005E15CBA</idno><date when="1981" year="1981">1981</date><idno type="doi">10.1016/0198-8859(81)90019-7</idno><idno type="url">https://api.istex.fr/document/1AE2024806226D4EB99DE8392DA35B4005E15CBA/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">002684</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Endothelial cell presentation of antigen to human T cells</title><author><name sortKey="Burger, Denis R" sort="Burger, Denis R" uniqKey="Burger D" first="Denis R." last="Burger">Denis R. Burger</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Ford, Dennis" sort="Ford, Dennis" uniqKey="Ford D" first="Dennis" last="Ford">Dennis Ford</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Mark Vetto, R" sort="Mark Vetto, R" uniqKey="Mark Vetto R" first="R." last="Mark Vetto">R. Mark Vetto</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Hamblin, Anne" sort="Hamblin, Anne" uniqKey="Hamblin A" first="Anne" last="Hamblin">Anne Hamblin</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Goldstein, Andrew" sort="Goldstein, Andrew" uniqKey="Goldstein A" first="Andrew" last="Goldstein">Andrew Goldstein</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Hubbard, Michael" sort="Hubbard, Michael" uniqKey="Hubbard M" first="Michael" last="Hubbard">Michael Hubbard</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author><author><name sortKey="Dumonde, D C" sort="Dumonde, D C" uniqKey="Dumonde D" first="D. C." last="Dumonde">D. C. Dumonde</name><affiliation><mods:affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Human Immunology</title><title level="j" type="abbrev">HIM</title><idno type="ISSN">0198-8859</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">3</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="209">209</biblScope><biblScope unit="page" to="230">230</biblScope></imprint><idno type="ISSN">0198-8859</idno></series><idno type="istex">1AE2024806226D4EB99DE8392DA35B4005E15CBA</idno><idno type="DOI">10.1016/0198-8859(81)90019-7</idno><idno type="PII">0198-8859(81)90019-7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0198-8859</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Activation of human T cells requires presentation of antigen by Ia (HLA-DR in man) bearing cells of the mononuclear phagocytic series (macrophages, MØ), and more recently Langerbans cells, dendritic cells, and vascular endothelial cells. Since T cells must cross endothelial barriers to enter extavasulcar tissues during immune reactions, we investigated the role of endothelial cells in antigen presentation. Endothelial cells were cultured from human umbilical veins and identified by classic morphology and specific markers (factor VIII related antigen, and so on). Antigen-pulsed endothelial cells were used to present antigen to MØ-depleted human T cells; activation was assessed by 3H-thymidine uptake. The HLA-DR compatible endothelial cells were as effective as MØ-in reconstituting MØ-depleted T-cell responses. The endothelial cell reconstituted responses were antigen specific, HLA-DR restricted, and blocked by monoclonal antibodies to HLA-DR framework structures. Moreover, the T-cell responses were clonal with respect to HLA-DR. A monoclonal antibody completely eliminated MØ reconstitution of the MØ-depleted response without diminution of endothelial cell reconstitution of the same response. Fibroblasts and smooth muscle cells cultured from the same umbilical veins could not reconstitute the MØ-depleted T-cell response. These data indicate that endothelial cells play an important and distinctive role in lymphocyte triggering.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Denis R. Burger</name><affiliations><json:string>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</json:string></affiliations></json:item><json:item><name>Dennis Ford</name><affiliations><json:string>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</json:string></affiliations></json:item><json:item><name>R. Mark Vetto</name><affiliations><json:string>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</json:string></affiliations></json:item><json:item><name>Anne Hamblin</name><affiliations><json:string>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</json:string></affiliations></json:item><json:item><name>Andrew Goldstein</name><affiliations><json:string>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</json:string></affiliations></json:item><json:item><name>Michael Hubbard</name><affiliations><json:string>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</json:string></affiliations></json:item><json:item><name>D.C. Dumonde</name><affiliations><json:string>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Ia : immune response associated</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CMI : cell-mediated immunity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>MNC : mononuclear cells</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PPD : purified protein derivative</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>MØ : cells of the mononuclear phagocytic series</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SK-SD : streptokinase-streptodornase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HLA : major histocompatibility complex of man</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>KLH : keyholelimpet hemocyanin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HCH : horsesshoe crab hemocyanin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SI : stimulation index</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>MLR : mixed lymphocyte reaction</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Activation of human T cells requires presentation of antigen by Ia (HLA-DR in man) bearing cells of the mononuclear phagocytic series (macrophages, MØ), and more recently Langerbans cells, dendritic cells, and vascular endothelial cells. Since T cells must cross endothelial barriers to enter extavasulcar tissues during immune reactions, we investigated the role of endothelial cells in antigen presentation. Endothelial cells were cultured from human umbilical veins and identified by classic morphology and specific markers (factor VIII related antigen, and so on). Antigen-pulsed endothelial cells were used to present antigen to MØ-depleted human T cells; activation was assessed by 3H-thymidine uptake. The HLA-DR compatible endothelial cells were as effective as MØ-in reconstituting MØ-depleted T-cell responses. The endothelial cell reconstituted responses were antigen specific, HLA-DR restricted, and blocked by monoclonal antibodies to HLA-DR framework structures. Moreover, the T-cell responses were clonal with respect to HLA-DR. A monoclonal antibody completely eliminated MØ reconstitution of the MØ-depleted response without diminution of endothelial cell reconstitution of the same response. Fibroblasts and smooth muscle cells cultured from the same umbilical veins could not reconstitute the MØ-depleted T-cell response. These data indicate that endothelial cells play an important and distinctive role in lymphocyte triggering.</abstract><qualityIndicators><score>7.364</score><pdfVersion>1.2</pdfVersion><pdfPageSize>339 x 504 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>11</keywordCount><abstractCharCount>1448</abstractCharCount><pdfWordCount>7507</pdfWordCount><pdfCharCount>44226</pdfCharCount><pdfPageCount>22</pdfPageCount><abstractWordCount>197</abstractWordCount></qualityIndicators><title>Endothelial cell presentation of antigen to human T cells</title><pii><json:string>0198-8859(81)90019-7</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>37</volume><pages><first>79</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Fed Proc</title></serie><host><volume>3</volume><pii><json:string>S0198-8859(00)X0125-5</json:string></pii><pages><last>230</last><first>209</first></pages><issn><json:string>0198-8859</json:string></issn><issue>3</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Human Immunology</title><publicationDate>1981</publicationDate></host><categories><wos><json:string>IMMUNOLOGY</json:string></wos></categories><publicationDate>1981</publicationDate><copyrightDate>1981</copyrightDate><doi><json:string>10.1016/0198-8859(81)90019-7</json:string></doi><id>1AE2024806226D4EB99DE8392DA35B4005E15CBA</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/1AE2024806226D4EB99DE8392DA35B4005E15CBA/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/1AE2024806226D4EB99DE8392DA35B4005E15CBA/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/1AE2024806226D4EB99DE8392DA35B4005E15CBA/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/1AE2024806226D4EB99DE8392DA35B4005E15CBA/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Endothelial cell presentation of antigen to human T cells</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1981</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Endothelial cell presentation of antigen to human T cells</title><author><persName><forename type="first">Denis R.</forename><surname>Burger</surname></persName><note type="biography">Address requests for reprints to Dr. Denis R. Burger, Veterans Administration Hospital, Portland Division, 3710 Southwest U.S., Veterans Hospital Road, P.O. Box 1034, Portland, OR 97201.</note><affiliation>Address requests for reprints to Dr. Denis R. Burger, Veterans Administration Hospital, Portland Division, 3710 Southwest U.S., Veterans Hospital Road, P.O. Box 1034, Portland, OR 97201.</affiliation><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation></author><author><persName><forename type="first">Dennis</forename><surname>Ford</surname></persName><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation></author><author><persName><forename type="first">R.</forename><surname>Mark Vetto</surname></persName><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation></author><author><persName><forename type="first">Anne</forename><surname>Hamblin</surname></persName><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation></author><author><persName><forename type="first">Andrew</forename><surname>Goldstein</surname></persName><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation></author><author><persName><forename type="first">Michael</forename><surname>Hubbard</surname></persName><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation></author><author><persName><forename type="first">D.C.</forename><surname>Dumonde</surname></persName><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation></author></analytic><monogr><title level="j">Human Immunology</title><title level="j" type="abbrev">HIM</title><idno type="pISSN">0198-8859</idno><idno type="PII">S0198-8859(00)X0125-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981"></date><biblScope unit="volume">3</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="209">209</biblScope><biblScope unit="page" to="230">230</biblScope></imprint></monogr><idno type="istex">1AE2024806226D4EB99DE8392DA35B4005E15CBA</idno><idno type="DOI">10.1016/0198-8859(81)90019-7</idno><idno type="PII">0198-8859(81)90019-7</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1981</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Activation of human T cells requires presentation of antigen by Ia (HLA-DR in man) bearing cells of the mononuclear phagocytic series (macrophages, MØ), and more recently Langerbans cells, dendritic cells, and vascular endothelial cells. Since T cells must cross endothelial barriers to enter extavasulcar tissues during immune reactions, we investigated the role of endothelial cells in antigen presentation. Endothelial cells were cultured from human umbilical veins and identified by classic morphology and specific markers (factor VIII related antigen, and so on). Antigen-pulsed endothelial cells were used to present antigen to MØ-depleted human T cells; activation was assessed by 3H-thymidine uptake. The HLA-DR compatible endothelial cells were as effective as MØ-in reconstituting MØ-depleted T-cell responses. The endothelial cell reconstituted responses were antigen specific, HLA-DR restricted, and blocked by monoclonal antibodies to HLA-DR framework structures. Moreover, the T-cell responses were clonal with respect to HLA-DR. A monoclonal antibody completely eliminated MØ reconstitution of the MØ-depleted response without diminution of endothelial cell reconstitution of the same response. Fibroblasts and smooth muscle cells cultured from the same umbilical veins could not reconstitute the MØ-depleted T-cell response. These data indicate that endothelial cells play an important and distinctive role in lymphocyte triggering.</p></abstract><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>Ia</term><term>immune response associated</term></item><item><term>CMI</term><term>cell-mediated immunity</term></item><item><term>MNC</term><term>mononuclear cells</term></item><item><term>PPD</term><term>purified protein derivative</term></item><item><term>MØ</term><term>cells of the mononuclear phagocytic series</term></item><item><term>SK-SD</term><term>streptokinase-streptodornase</term></item><item><term>HLA</term><term>major histocompatibility complex of man</term></item><item><term>KLH</term><term>keyholelimpet hemocyanin</term></item><item><term>HCH</term><term>horsesshoe crab hemocyanin</term></item><item><term>SI</term><term>stimulation index</term></item><item><term>MLR</term><term>mixed lymphocyte reaction</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1981">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>HIM</jid><aid>81900197</aid><ce:pii>0198-8859(81)90019-7</ce:pii><ce:doi>10.1016/0198-8859(81)90019-7</ce:doi><ce:copyright type="unknown" year="1981"></ce:copyright></item-info><head><ce:title>Endothelial cell presentation of antigen to human T cells</ce:title><ce:author-group><ce:author><ce:given-name>Denis R.</ce:given-name><ce:surname>Burger</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Dennis</ce:given-name><ce:surname>Ford</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>R.</ce:given-name><ce:surname>Mark Vetto</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Anne</ce:given-name><ce:surname>Hamblin</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Andrew</ce:given-name><ce:surname>Goldstein</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Michael</ce:given-name><ce:surname>Hubbard</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>D.C.</ce:given-name><ce:surname>Dumonde</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Departments of Microbiology and Immunology, University of Oregon Health Sciences Center, Portland, UK</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Department of Surgery, University of Oregon Health Sciences Center, Portland, UK</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>Immunologic Associates, Portland, Oregon, UK</ce:textfn></ce:affiliation><ce:affiliation id="AFF5"><ce:label>e</ce:label><ce:textfn>Department of Immunology, St. Thomas' Hospital, London, UK</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Address requests for reprints to Dr. Denis R. Burger, Veterans Administration Hospital, Portland Division, 3710 Southwest U.S., Veterans Hospital Road, P.O. Box 1034, Portland, OR 97201.</ce:text></ce:correspondence></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Activation of human T cells requires presentation of antigen by Ia (HLA-DR in man) bearing cells of the mononuclear phagocytic series (macrophages, MØ), and more recently Langerbans cells, dendritic cells, and vascular endothelial cells. Since T cells must cross endothelial barriers to enter extavasulcar tissues during immune reactions, we investigated the role of endothelial cells in antigen presentation. Endothelial cells were cultured from human umbilical veins and identified by classic morphology and specific markers (factor VIII related antigen, and so on). Antigen-pulsed endothelial cells were used to present antigen to MØ-depleted human T cells; activation was assessed by <ce:sup loc="pre">3</ce:sup>H-thymidine uptake. The HLA-DR compatible endothelial cells were as effective as MØ-in reconstituting MØ-depleted T-cell responses. The endothelial cell reconstituted responses were antigen specific, HLA-DR restricted, and blocked by monoclonal antibodies to HLA-DR framework structures. Moreover, the T-cell responses were clonal with respect to HLA-DR. A monoclonal antibody completely eliminated MØ reconstitution of the MØ-depleted response without diminution of endothelial cell reconstitution of the same response. Fibroblasts and smooth muscle cells cultured from the same umbilical veins could not reconstitute the MØ-depleted T-cell response. These data indicate that endothelial cells play an important and distinctive role in lymphocyte triggering.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>Ia</ce:text><ce:keyword><ce:text>immune response associated</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>CMI</ce:text><ce:keyword><ce:text>cell-mediated immunity</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>MNC</ce:text><ce:keyword><ce:text>mononuclear cells</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PPD</ce:text><ce:keyword><ce:text>purified protein derivative</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>MØ</ce:text><ce:keyword><ce:text>cells of the mononuclear phagocytic series</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SK-SD</ce:text><ce:keyword><ce:text>streptokinase-streptodornase</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>HLA</ce:text><ce:keyword><ce:text>major histocompatibility complex of man</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>KLH</ce:text><ce:keyword><ce:text>keyholelimpet hemocyanin</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>HCH</ce:text><ce:keyword><ce:text>horsesshoe crab hemocyanin</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SI</ce:text><ce:keyword><ce:text>stimulation index</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>MLR</ce:text><ce:keyword><ce:text>mixed lymphocyte reaction</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Endothelial cell presentation of antigen to human T cells</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Endothelial cell presentation of antigen to human T cells</title></titleInfo><name type="personal"><namePart type="given">Denis R.</namePart><namePart type="family">Burger</namePart><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation><description>Address requests for reprints to Dr. Denis R. Burger, Veterans Administration Hospital, Portland Division, 3710 Southwest U.S., Veterans Hospital Road, P.O. Box 1034, Portland, OR 97201.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dennis</namePart><namePart type="family">Ford</namePart><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Mark Vetto</namePart><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Anne</namePart><namePart type="family">Hamblin</namePart><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Andrew</namePart><namePart type="family">Goldstein</namePart><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Michael</namePart><namePart type="family">Hubbard</namePart><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.C.</namePart><namePart type="family">Dumonde</namePart><affiliation>Surgical Research Laboratory, Veterans Aministration Medical Center, Portland, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued><copyrightDate encoding="w3cdtf">1981</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Activation of human T cells requires presentation of antigen by Ia (HLA-DR in man) bearing cells of the mononuclear phagocytic series (macrophages, MØ), and more recently Langerbans cells, dendritic cells, and vascular endothelial cells. Since T cells must cross endothelial barriers to enter extavasulcar tissues during immune reactions, we investigated the role of endothelial cells in antigen presentation. Endothelial cells were cultured from human umbilical veins and identified by classic morphology and specific markers (factor VIII related antigen, and so on). Antigen-pulsed endothelial cells were used to present antigen to MØ-depleted human T cells; activation was assessed by 3H-thymidine uptake. The HLA-DR compatible endothelial cells were as effective as MØ-in reconstituting MØ-depleted T-cell responses. The endothelial cell reconstituted responses were antigen specific, HLA-DR restricted, and blocked by monoclonal antibodies to HLA-DR framework structures. Moreover, the T-cell responses were clonal with respect to HLA-DR. A monoclonal antibody completely eliminated MØ reconstitution of the MØ-depleted response without diminution of endothelial cell reconstitution of the same response. Fibroblasts and smooth muscle cells cultured from the same umbilical veins could not reconstitute the MØ-depleted T-cell response. These data indicate that endothelial cells play an important and distinctive role in lymphocyte triggering.</abstract><subject><genre>Abbreviations</genre><topic>Ia : immune response associated</topic><topic>CMI : cell-mediated immunity</topic><topic>MNC : mononuclear cells</topic><topic>PPD : purified protein derivative</topic><topic>MØ : cells of the mononuclear phagocytic series</topic><topic>SK-SD : streptokinase-streptodornase</topic><topic>HLA : major histocompatibility complex of man</topic><topic>KLH : keyholelimpet hemocyanin</topic><topic>HCH : horsesshoe crab hemocyanin</topic><topic>SI : stimulation index</topic><topic>MLR : mixed lymphocyte reaction</topic></subject><relatedItem type="host"><titleInfo><title>Human Immunology</title></titleInfo><titleInfo type="abbreviated"><title>HIM</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">198111</dateIssued></originInfo><identifier type="ISSN">0198-8859</identifier><identifier type="PII">S0198-8859(00)X0125-5</identifier><part><date>198111</date><detail type="volume"><number>3</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue pages"><start>187</start><end>275</end></extent><extent unit="pages"><start>209</start><end>230</end></extent></part></relatedItem><identifier type="istex">1AE2024806226D4EB99DE8392DA35B4005E15CBA</identifier><identifier type="DOI">10.1016/0198-8859(81)90019-7</identifier><identifier type="PII">0198-8859(81)90019-7</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/1AE2024806226D4EB99DE8392DA35B4005E15CBA/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">IMMUNOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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