Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients
Identifieur interne : 000B53 ( Istex/Corpus ); précédent : 000B52; suivant : 000B54Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients
Auteurs : Anna Linda Zignego ; Frederic Dubois ; Didier Samuel ; Urania Georgopoulou ; Michel Reynes ; Paolo Gentilini ; Alain Bismuth ; Jean-Pierre Benhamou ; Stephanos J. Hadziyannis ; Henry Bismuth ; Christian BréchotSource :
- Journal of Hepatology [ 0168-8278 ] ; 1990.
Abstract
We have investigated the usefulness of serum hepatitis delta virus (HDV) RNA detection using a slot hybridization analysis of serum samples from ten patients with acute hepatitis and delta markers (group I), from 28 patients with chronic delta hepatitis (group II) and from seven liver graft recipients with hepatitis B virus (HBV) and HDV related cirrhosis or fulminant hepatitis (group III). The slot-blots were hybridized with both HDV-complementary DNA and single-stranded RNA probes. With the single-stranded RNA probe, HDV RNA was detected in the first serum sample available in 9 10 of the patients with acute hepatitis (group I). In addition, HDV RNA was detected in 8 9 and 7 8 of the samples obtained within and after 1 month of the onset of hepatitis. Five of the ten patients scored positive for HDV RNA and negative for hepatitis delta antigen (HDAg) while one was negative for HDV RNA and positive for HDAg. The same RNA probe enabled the detection of serum HDV RNA in 21 28 chronic hepatitis patients (liver HDAg and/or IgM anti-HD positive) (group II). Among the liver graft recipients (group III), 7 7 had a recurrent delta infection. Serum HDAg, liver HDAg and anti-HD IgM were identified in 3 7, 6 7, and, 5 7 of the patients, respectively. HDV RNA was detected in the seven patients with either persistent ( 4 7) or transient ( 3 7) positivity. In addition, HBsAg and HBV RNA were persistently shown in 4 7 patients with continuous HDV replication. In the remaining three patients, HDV RNA was detectable despite the absence of HBsAg. Liver histology showed features of chronic hepatitis in 3 7 with persistent detection of serum HDV RNA. The results of this study indicate that HDV RNA detection in serum, through identification of infectious particles, provide useful information for the appraisal of HDV-related liver disease.
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DOI: 10.1016/0168-8278(90)90279-Z
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Hadziyannis</name><affiliation><mods:affiliation>Academic Department of Medicine, Hippokration General Hospital, Athens, Greece</mods:affiliation></affiliation></author><author><name sortKey="Bismuth, Henry" sort="Bismuth, Henry" uniqKey="Bismuth H" first="Henry" last="Bismuth">Henry Bismuth</name><affiliation><mods:affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</mods:affiliation></affiliation></author><author><name sortKey="Brechot, Christian" sort="Brechot, Christian" uniqKey="Brechot C" first="Christian" last="Bréchot">Christian Bréchot</name><affiliation><mods:affiliation>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Hepatology</title><title level="j" type="abbrev">JHEPAT</title><idno type="ISSN">0168-8278</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="102">102</biblScope><biblScope unit="page" to="110">110</biblScope></imprint><idno type="ISSN">0168-8278</idno></series><idno type="istex">13CD6AE20C71A081AC49446B13992330677C9414</idno><idno type="DOI">10.1016/0168-8278(90)90279-Z</idno><idno type="PII">0168-8278(90)90279-Z</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-8278</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have investigated the usefulness of serum hepatitis delta virus (HDV) RNA detection using a slot hybridization analysis of serum samples from ten patients with acute hepatitis and delta markers (group I), from 28 patients with chronic delta hepatitis (group II) and from seven liver graft recipients with hepatitis B virus (HBV) and HDV related cirrhosis or fulminant hepatitis (group III). The slot-blots were hybridized with both HDV-complementary DNA and single-stranded RNA probes. With the single-stranded RNA probe, HDV RNA was detected in the first serum sample available in 9 10 of the patients with acute hepatitis (group I). In addition, HDV RNA was detected in 8 9 and 7 8 of the samples obtained within and after 1 month of the onset of hepatitis. Five of the ten patients scored positive for HDV RNA and negative for hepatitis delta antigen (HDAg) while one was negative for HDV RNA and positive for HDAg. The same RNA probe enabled the detection of serum HDV RNA in 21 28 chronic hepatitis patients (liver HDAg and/or IgM anti-HD positive) (group II). Among the liver graft recipients (group III), 7 7 had a recurrent delta infection. Serum HDAg, liver HDAg and anti-HD IgM were identified in 3 7, 6 7, and, 5 7 of the patients, respectively. HDV RNA was detected in the seven patients with either persistent ( 4 7) or transient ( 3 7) positivity. In addition, HBsAg and HBV RNA were persistently shown in 4 7 patients with continuous HDV replication. In the remaining three patients, HDV RNA was detectable despite the absence of HBsAg. Liver histology showed features of chronic hepatitis in 3 7 with persistent detection of serum HDV RNA. The results of this study indicate that HDV RNA detection in serum, through identification of infectious particles, provide useful information for the appraisal of HDV-related liver disease.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Anna Linda Zignego</name><affiliations><json:string>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</json:string></affiliations></json:item><json:item><name>Frederic Dubois</name><affiliations><json:string>Groupe d'Analyse Structurale des Antigènes (CNRS URA 225) CHU Bretonneau, Tours, France</json:string></affiliations></json:item><json:item><name>Didier Samuel</name><affiliations><json:string>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</json:string></affiliations></json:item><json:item><name>Urania Georgopoulou</name><affiliations><json:string>INSERM U 75 CHU Necker and Unité d'Hépatologie Hôpital Laennec, Paris, France</json:string></affiliations></json:item><json:item><name>Michel Reynes</name><affiliations><json:string>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</json:string></affiliations></json:item><json:item><name>Paolo Gentilini</name><affiliations><json:string>Istituto di Clinica Medica II, University of Florence, School of Medicine, Florence, Italy</json:string></affiliations></json:item><json:item><name>Alain Bismuth</name><affiliations><json:string>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</json:string></affiliations></json:item><json:item><name>Jean-Pierre Benhamou</name><affiliations><json:string>Unité d'Hépatologie, Hôpital Beaujon, Paris, France</json:string></affiliations></json:item><json:item><name>Stephanos J. Hadziyannis</name><affiliations><json:string>Academic Department of Medicine, Hippokration General Hospital, Athens, Greece</json:string></affiliations></json:item><json:item><name>Henry Bismuth</name><affiliations><json:string>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</json:string></affiliations></json:item><json:item><name>Christian Bréchot</name><affiliations><json:string>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><abstract>We have investigated the usefulness of serum hepatitis delta virus (HDV) RNA detection using a slot hybridization analysis of serum samples from ten patients with acute hepatitis and delta markers (group I), from 28 patients with chronic delta hepatitis (group II) and from seven liver graft recipients with hepatitis B virus (HBV) and HDV related cirrhosis or fulminant hepatitis (group III). The slot-blots were hybridized with both HDV-complementary DNA and single-stranded RNA probes. With the single-stranded RNA probe, HDV RNA was detected in the first serum sample available in 9 10 of the patients with acute hepatitis (group I). In addition, HDV RNA was detected in 8 9 and 7 8 of the samples obtained within and after 1 month of the onset of hepatitis. Five of the ten patients scored positive for HDV RNA and negative for hepatitis delta antigen (HDAg) while one was negative for HDV RNA and positive for HDAg. The same RNA probe enabled the detection of serum HDV RNA in 21 28 chronic hepatitis patients (liver HDAg and/or IgM anti-HD positive) (group II). Among the liver graft recipients (group III), 7 7 had a recurrent delta infection. Serum HDAg, liver HDAg and anti-HD IgM were identified in 3 7, 6 7, and, 5 7 of the patients, respectively. HDV RNA was detected in the seven patients with either persistent ( 4 7) or transient ( 3 7) positivity. In addition, HBsAg and HBV RNA were persistently shown in 4 7 patients with continuous HDV replication. In the remaining three patients, HDV RNA was detectable despite the absence of HBsAg. Liver histology showed features of chronic hepatitis in 3 7 with persistent detection of serum HDV RNA. The results of this study indicate that HDV RNA detection in serum, through identification of infectious particles, provide useful information for the appraisal of HDV-related liver disease.</abstract><qualityIndicators><score>6.337</score><pdfVersion>1.2</pdfVersion><pdfPageSize>360 x 468 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1849</abstractCharCount><pdfWordCount>3337</pdfWordCount><pdfCharCount>21075</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>308</abstractWordCount></qualityIndicators><title>Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients</title><pii><json:string>0168-8278(90)90279-Z</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>Vol. VIII</volume><pages><last>431</last><first>417</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Progress in Liver 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level="a">Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1990</date></publicationStmt><notesStmt><note type="content">Section title: Regular paper</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients</title><author><persName><forename type="first">Anna Linda</forename><surname>Zignego</surname></persName><note type="correspondence"><p>Correspondence: Anna Linda Zignego, M.D., Institut Pasteur, Unité de Recombinaison et Expression Génétique, 28, rue du Dr. Roux, 75015 Paris Cedex, France.</p></note><affiliation>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</affiliation></author><author><persName><forename type="first">Frederic</forename><surname>Dubois</surname></persName><affiliation>Groupe d'Analyse Structurale des Antigènes (CNRS URA 225) CHU Bretonneau, Tours, France</affiliation></author><author><persName><forename type="first">Didier</forename><surname>Samuel</surname></persName><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation></author><author><persName><forename type="first">Urania</forename><surname>Georgopoulou</surname></persName><affiliation>INSERM U 75 CHU Necker and Unité d'Hépatologie Hôpital Laennec, Paris, France</affiliation></author><author><persName><forename type="first">Michel</forename><surname>Reynes</surname></persName><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation></author><author><persName><forename type="first">Paolo</forename><surname>Gentilini</surname></persName><affiliation>Istituto di Clinica Medica II, University of Florence, School of Medicine, Florence, Italy</affiliation></author><author><persName><forename type="first">Alain</forename><surname>Bismuth</surname></persName><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation></author><author><persName><forename type="first">Jean-Pierre</forename><surname>Benhamou</surname></persName><affiliation>Unité d'Hépatologie, Hôpital Beaujon, Paris, France</affiliation></author><author><persName><forename type="first">Stephanos J.</forename><surname>Hadziyannis</surname></persName><affiliation>Academic Department of Medicine, Hippokration General Hospital, Athens, Greece</affiliation></author><author><persName><forename type="first">Henry</forename><surname>Bismuth</surname></persName><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation></author><author><persName><forename type="first">Christian</forename><surname>Bréchot</surname></persName><affiliation>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</affiliation></author></analytic><monogr><title level="j">Journal of Hepatology</title><title level="j" type="abbrev">JHEPAT</title><idno type="pISSN">0168-8278</idno><idno type="PII">S0168-8278(00)X0142-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990"></date><biblScope unit="volume">11</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="102">102</biblScope><biblScope unit="page" to="110">110</biblScope></imprint></monogr><idno type="istex">13CD6AE20C71A081AC49446B13992330677C9414</idno><idno type="DOI">10.1016/0168-8278(90)90279-Z</idno><idno type="PII">0168-8278(90)90279-Z</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1990</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>We have investigated the usefulness of serum hepatitis delta virus (HDV) RNA detection using a slot hybridization analysis of serum samples from ten patients with acute hepatitis and delta markers (group I), from 28 patients with chronic delta hepatitis (group II) and from seven liver graft recipients with hepatitis B virus (HBV) and HDV related cirrhosis or fulminant hepatitis (group III). The slot-blots were hybridized with both HDV-complementary DNA and single-stranded RNA probes. With the single-stranded RNA probe, HDV RNA was detected in the first serum sample available in 9 10 of the patients with acute hepatitis (group I). In addition, HDV RNA was detected in 8 9 and 7 8 of the samples obtained within and after 1 month of the onset of hepatitis. Five of the ten patients scored positive for HDV RNA and negative for hepatitis delta antigen (HDAg) while one was negative for HDV RNA and positive for HDAg. The same RNA probe enabled the detection of serum HDV RNA in 21 28 chronic hepatitis patients (liver HDAg and/or IgM anti-HD positive) (group II). Among the liver graft recipients (group III), 7 7 had a recurrent delta infection. Serum HDAg, liver HDAg and anti-HD IgM were identified in 3 7, 6 7, and, 5 7 of the patients, respectively. HDV RNA was detected in the seven patients with either persistent ( 4 7) or transient ( 3 7) positivity. In addition, HBsAg and HBV RNA were persistently shown in 4 7 patients with continuous HDV replication. In the remaining three patients, HDV RNA was detectable despite the absence of HBsAg. Liver histology showed features of chronic hepatitis in 3 7 with persistent detection of serum HDV RNA. The results of this study indicate that HDV RNA detection in serum, through identification of infectious particles, provide useful information for the appraisal of HDV-related liver disease.</p></abstract></profileDesc><revisionDesc><change when="1990-03-20">Registration</change><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JHEPAT</jid><aid>9090279Z</aid><ce:pii>0168-8278(90)90279-Z</ce:pii><ce:doi>10.1016/0168-8278(90)90279-Z</ce:doi><ce:copyright type="unknown" year="1990"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular paper</ce:textfn></ce:dochead><ce:title>Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients</ce:title><ce:author-group><ce:author><ce:given-name>Anna Linda</ce:given-name><ce:surname>Zignego</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Frederic</ce:given-name><ce:surname>Dubois</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Didier</ce:given-name><ce:surname>Samuel</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Urania</ce:given-name><ce:surname>Georgopoulou</ce:surname><ce:cross-ref refid="AFF5"><ce:sup>5</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Michel</ce:given-name><ce:surname>Reynes</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Paolo</ce:given-name><ce:surname>Gentilini</ce:surname><ce:cross-ref refid="AFF6"><ce:sup>6</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Alain</ce:given-name><ce:surname>Bismuth</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jean-Pierre</ce:given-name><ce:surname>Benhamou</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>4</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Stephanos J.</ce:given-name><ce:surname>Hadziyannis</ce:surname><ce:cross-ref refid="AFF7"><ce:sup>7</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Henry</ce:given-name><ce:surname>Bismuth</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Christian</ce:given-name><ce:surname>Bréchot</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF6"><ce:sup>6</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>1</ce:label><ce:textfn>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>2</ce:label><ce:textfn>Groupe d'Analyse Structurale des Antigènes (CNRS URA 225) CHU Bretonneau, Tours, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>3</ce:label><ce:textfn>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>4</ce:label><ce:textfn>Unité d'Hépatologie, Hôpital Beaujon, Paris, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF5"><ce:label>5</ce:label><ce:textfn>INSERM U 75 CHU Necker and Unité d'Hépatologie Hôpital Laennec, Paris, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF6"><ce:label>6</ce:label><ce:textfn>Istituto di Clinica Medica II, University of Florence, School of Medicine, Florence, Italy</ce:textfn></ce:affiliation><ce:affiliation id="AFF7"><ce:label>7</ce:label><ce:textfn>Academic Department of Medicine, Hippokration General Hospital, Athens, Greece</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence: Anna Linda Zignego, M.D., Institut Pasteur, Unité de Recombinaison et Expression Génétique, 28, rue du Dr. Roux, 75015 Paris Cedex, France.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="31" month="7" year="1989"></ce:date-received><ce:date-accepted day="20" month="3" year="1990"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We have investigated the usefulness of serum hepatitis delta virus (HDV) RNA detection using a slot hybridization analysis of serum samples from ten patients with acute hepatitis and delta markers (group I), from 28 patients with chronic delta hepatitis (group II) and from seven liver graft recipients with hepatitis B virus (HBV) and HDV related cirrhosis or fulminant hepatitis (group III). The slot-blots were hybridized with both HDV-complementary DNA and single-stranded RNA probes. With the single-stranded RNA probe, HDV RNA was detected in the first serum sample available in <math altimg="si1.gif"><fr shape="sol"><nu>9</nu><de>10</de></fr></math> of the patients with acute hepatitis (group I). In addition, HDV RNA was detected in <math altimg="si2.gif"><fr shape="sol"><nu>8</nu><de>9</de></fr></math> and <math altimg="si3.gif"><fr shape="sol"><nu>7</nu><de>8</de></fr></math> of the samples obtained within and after 1 month of the onset of hepatitis. Five of the ten patients scored positive for HDV RNA and negative for hepatitis delta antigen (HDAg) while one was negative for HDV RNA and positive for HDAg. The same RNA probe enabled the detection of serum HDV RNA in <math altimg="si4.gif"><fr shape="sol"><nu>21</nu><de>28</de></fr></math> chronic hepatitis patients (liver HDAg and/or IgM anti-HD positive) (group II). Among the liver graft recipients (group III), <math altimg="si5.gif"><fr shape="sol"><nu>7</nu><de>7</de></fr></math> had a recurrent delta infection. Serum HDAg, liver HDAg and anti-HD IgM were identified in <math altimg="si6.gif"><fr shape="sol"><nu>3</nu><de>7</de></fr>, <fr shape="sol"><nu>6</nu><de>7</de></fr>, <rm>and</rm>, <fr shape="sol"><nu>5</nu><de>7</de></fr></math> of the patients, respectively. HDV RNA was detected in the seven patients with either persistent (<math altimg="si7.gif"><fr shape="sol"><nu>4</nu><de>7</de></fr></math>) or transient (<math altimg="si8.gif"><fr shape="sol"><nu>3</nu><de>7</de></fr></math>) positivity. In addition, HBsAg and HBV RNA were persistently shown in <math altimg="si9.gif"><fr shape="sol"><nu>4</nu><de>7</de></fr></math> patients with continuous HDV replication. In the remaining three patients, HDV RNA was detectable despite the absence of HBsAg. Liver histology showed features of chronic hepatitis in <math altimg="si10.gif"><fr shape="sol"><nu>3</nu><de>7</de></fr></math> with persistent detection of serum HDV RNA. The results of this study indicate that HDV RNA detection in serum, through identification of infectious particles, provide useful information for the appraisal of HDV-related liver disease.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Serum hepatitis delta virus RNA in patients with delta hepatitis and in liver graft recipients</title></titleInfo><name type="personal"><namePart type="given">Anna Linda</namePart><namePart type="family">Zignego</namePart><affiliation>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</affiliation><description>Correspondence: Anna Linda Zignego, M.D., Institut Pasteur, Unité de Recombinaison et Expression Génétique, 28, rue du Dr. Roux, 75015 Paris Cedex, France.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frederic</namePart><namePart type="family">Dubois</namePart><affiliation>Groupe d'Analyse Structurale des Antigènes (CNRS URA 225) CHU Bretonneau, Tours, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Didier</namePart><namePart type="family">Samuel</namePart><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Urania</namePart><namePart type="family">Georgopoulou</namePart><affiliation>INSERM U 75 CHU Necker and Unité d'Hépatologie Hôpital Laennec, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Michel</namePart><namePart type="family">Reynes</namePart><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Paolo</namePart><namePart type="family">Gentilini</namePart><affiliation>Istituto di Clinica Medica II, University of Florence, School of Medicine, Florence, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alain</namePart><namePart type="family">Bismuth</namePart><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean-Pierre</namePart><namePart type="family">Benhamou</namePart><affiliation>Unité d'Hépatologie, Hôpital Beaujon, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephanos J.</namePart><namePart type="family">Hadziyannis</namePart><affiliation>Academic Department of Medicine, Hippokration General Hospital, Athens, Greece</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Henry</namePart><namePart type="family">Bismuth</namePart><affiliation>Service de Chirurgie Digestive, Laboratoire d'Anatomopathologie, Banque du Sang et Laboratoire de Bactériologie, Hôpital Paul Brousse, Villejuif, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christian</namePart><namePart type="family">Bréchot</namePart><affiliation>Unité de Recombinaison et Expression Génétique (INSERM U 163/CNRS UA 271) Institut Pasteur, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued><dateValid encoding="w3cdtf">1990-03-20</dateValid><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">We have investigated the usefulness of serum hepatitis delta virus (HDV) RNA detection using a slot hybridization analysis of serum samples from ten patients with acute hepatitis and delta markers (group I), from 28 patients with chronic delta hepatitis (group II) and from seven liver graft recipients with hepatitis B virus (HBV) and HDV related cirrhosis or fulminant hepatitis (group III). The slot-blots were hybridized with both HDV-complementary DNA and single-stranded RNA probes. With the single-stranded RNA probe, HDV RNA was detected in the first serum sample available in 9 10 of the patients with acute hepatitis (group I). In addition, HDV RNA was detected in 8 9 and 7 8 of the samples obtained within and after 1 month of the onset of hepatitis. Five of the ten patients scored positive for HDV RNA and negative for hepatitis delta antigen (HDAg) while one was negative for HDV RNA and positive for HDAg. The same RNA probe enabled the detection of serum HDV RNA in 21 28 chronic hepatitis patients (liver HDAg and/or IgM anti-HD positive) (group II). Among the liver graft recipients (group III), 7 7 had a recurrent delta infection. Serum HDAg, liver HDAg and anti-HD IgM were identified in 3 7, 6 7, and, 5 7 of the patients, respectively. HDV RNA was detected in the seven patients with either persistent ( 4 7) or transient ( 3 7) positivity. In addition, HBsAg and HBV RNA were persistently shown in 4 7 patients with continuous HDV replication. In the remaining three patients, HDV RNA was detectable despite the absence of HBsAg. Liver histology showed features of chronic hepatitis in 3 7 with persistent detection of serum HDV RNA. The results of this study indicate that HDV RNA detection in serum, through identification of infectious particles, provide useful information for the appraisal of HDV-related liver disease.</abstract><note type="content">Section title: Regular paper</note><relatedItem type="host"><titleInfo><title>Journal of Hepatology</title></titleInfo><titleInfo type="abbreviated"><title>JHEPAT</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199007</dateIssued></originInfo><identifier type="ISSN">0168-8278</identifier><identifier type="PII">S0168-8278(00)X0142-0</identifier><part><date>199007</date><detail type="volume"><number>11</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>132</end></extent><extent unit="pages"><start>102</start><end>110</end></extent></part></relatedItem><identifier type="istex">13CD6AE20C71A081AC49446B13992330677C9414</identifier><identifier type="DOI">10.1016/0168-8278(90)90279-Z</identifier><identifier type="PII">0168-8278(90)90279-Z</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/13CD6AE20C71A081AC49446B13992330677C9414/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">GASTROENTEROLOGY & HEPATOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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