Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis.
Identifieur interne : 000341 ( PubMed/Checkpoint ); précédent : 000340; suivant : 000342Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis.
Auteurs : Hélène Manduzio [France] ; Tiphaine Monsinjon ; Béatrice Rocher ; François Leboulenger ; Camille GalapSource :
- Aquatic toxicology (Amsterdam, Netherlands) [ 0166-445X ] ; 2003.
English descriptors
- KwdEn :
- Animals, Bivalvia (enzymology), Copper (toxicity), Environmental Exposure (adverse effects), Environmental Monitoring (methods), Enzyme Induction (physiology), Isoenzymes (biosynthesis), Isoenzymes (isolation & purification), Oxidative Stress, Seawater (chemistry), Superoxide Dismutase (biosynthesis), Superoxide Dismutase (isolation & purification), Water Pollutants, Chemical (analysis), Water Pollutants, Chemical (toxicity).
- MESH :
- chemical , analysis : Water Pollutants, Chemical.
- chemical , biosynthesis : Isoenzymes, Superoxide Dismutase.
- chemical , isolation & purification : Isoenzymes, Superoxide Dismutase.
- chemical , toxicity : Copper, Water Pollutants, Chemical.
- adverse effects : Environmental Exposure.
- chemistry : Seawater.
- enzymology : Bivalvia.
- methods : Environmental Monitoring.
- physiology : Enzyme Induction.
- Animals, Oxidative Stress.
Abstract
Aerobic organisms are protected against oxidative stress by antioxidant systems which mobilise enzymes such as the Cu/Zn superoxide dismutase (Cu/Zn-SOD) which transfers O2(.-) to H2O2. In this paper, we report the characterization of three isoforms of Cu/Zn-SOD in the blue mussel Mytilus edulis and we show that one of these isoforms is strongly inducible. Cytosolic extracts of digestive gland and gills from adult blue mussels were analysed by polyacrylamide gel electrophoresis or isoelectric focusing followed by in situ staining for SOD activity. Two main bands of Cu/Zn-SOD were obtained at pI 4.7 and 4.6 corresponding to native apparent molecular weight values of 205 and 155 kDa. Blue mussels from chemically contaminated area in Le Havre harbour exhibited a third Cu/Zn-SOD isoform characterized by a more acidic isoelectric point (pI 4.55) and a native apparent molecular weight of 130 kDa. When maintained in clean marine water, mussels from this area showed a transitory decrease in total SOD activity accompanied by the disappearance of the SOD-3 band. Conversely, the exposure (4 and 8 h, and 3 and 7 days) of control blue mussels to copper (25 microg l(-1)) markedly increased SOD-3 band while the total SOD activity did not systematically change. Taken together our results suggest that the variations of SOD expression pattern in Mytihus edulis could be used as a tool for the marine environment monitoring.
PubMed: 12820627
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xml:lang="en"><term>Isoenzymes</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Copper</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" qualifier="adverse effects" xml:lang="en"><term>Environmental Exposure</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Seawater</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bivalvia</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Environmental Monitoring</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Enzyme Induction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Oxidative Stress</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Aerobic organisms are protected against oxidative stress by antioxidant systems which mobilise enzymes such as the Cu/Zn superoxide dismutase (Cu/Zn-SOD) which transfers O2(.-) to H2O2. In this paper, we report the characterization of three isoforms of Cu/Zn-SOD in the blue mussel Mytilus edulis and we show that one of these isoforms is strongly inducible. Cytosolic extracts of digestive gland and gills from adult blue mussels were analysed by polyacrylamide gel electrophoresis or isoelectric focusing followed by in situ staining for SOD activity. Two main bands of Cu/Zn-SOD were obtained at pI 4.7 and 4.6 corresponding to native apparent molecular weight values of 205 and 155 kDa. Blue mussels from chemically contaminated area in Le Havre harbour exhibited a third Cu/Zn-SOD isoform characterized by a more acidic isoelectric point (pI 4.55) and a native apparent molecular weight of 130 kDa. When maintained in clean marine water, mussels from this area showed a transitory decrease in total SOD activity accompanied by the disappearance of the SOD-3 band. Conversely, the exposure (4 and 8 h, and 3 and 7 days) of control blue mussels to copper (25 microg l(-1)) markedly increased SOD-3 band while the total SOD activity did not systematically change. Taken together our results suggest that the variations of SOD expression pattern in Mytihus edulis could be used as a tool for the marine environment monitoring.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12820627</PMID><DateCreated><Year>2003</Year><Month>6</Month><Day>23</Day></DateCreated><DateCompleted><Year>2003</Year><Month>08</Month><Day>20</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-445X</ISSN><JournalIssue CitedMedium="Print"><Volume>64</Volume><Issue>1</Issue><PubDate><Year>2003</Year><Month>Jun</Month><Day>19</Day></PubDate></JournalIssue><Title>Aquatic toxicology (Amsterdam, Netherlands)</Title><ISOAbbreviation>Aquat. 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Blue mussels from chemically contaminated area in Le Havre harbour exhibited a third Cu/Zn-SOD isoform characterized by a more acidic isoelectric point (pI 4.55) and a native apparent molecular weight of 130 kDa. When maintained in clean marine water, mussels from this area showed a transitory decrease in total SOD activity accompanied by the disappearance of the SOD-3 band. Conversely, the exposure (4 and 8 h, and 3 and 7 days) of control blue mussels to copper (25 microg l(-1)) markedly increased SOD-3 band while the total SOD activity did not systematically change. 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Havre</li></settlement></list><tree><noCountry><name sortKey="Galap, Camille" sort="Galap, Camille" uniqKey="Galap C" first="Camille" last="Galap">Camille Galap</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name><name sortKey="Monsinjon, Tiphaine" sort="Monsinjon, Tiphaine" uniqKey="Monsinjon T" first="Tiphaine" last="Monsinjon">Tiphaine Monsinjon</name><name sortKey="Rocher, Beatrice" sort="Rocher, Beatrice" uniqKey="Rocher B" first="Béatrice" last="Rocher">Béatrice Rocher</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Manduzio, Helene" sort="Manduzio, Helene" uniqKey="Manduzio H" first="Hélène" last="Manduzio">Hélène Manduzio</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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