ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria
Identifieur interne : 001770 ( Pmc/Curation ); précédent : 001769; suivant : 001771ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria
Auteurs : Andrew N. Keller [Australie] ; Xiao Yang [Australie] ; Jana Wiedermannová [République tchèque] ; Olivier Delumeau [France] ; Libor Krásn [République tchèque] ; Peter J. Lewis [Australie]Source :
- Journal of Bacteriology [ 0021-9193 ] ; 2014.
Abstract
RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T
Url:
DOI: 10.1128/JB.02020-14
PubMed: 25092033
PubMed Central: 4187704
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<front><div type="abstract" xml:lang="en"><p>RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T <named-content content-type="genus-species">Firmicutes</named-content>
phylum of Gram-positive bacteria and have named it ε. Previously ε had been identified as a small protein (ω<sub>1</sub>
) that copurified with RNA polymerase. We have solved the structure of ε by X-ray crystallography and show that it is not an ω subunit. Rather, ε bears remarkable similarity to the Gp2 family of phage proteins involved in the inhibition of host cell transcription following infection. Deletion of ε shows no phenotype and has no effect on the transcriptional profile of the cell. Determination of the location of ε within the assembly of RNA polymerase core by single-particle analysis suggests that it binds toward the downstream side of the DNA binding cleft. Due to the structural similarity of ε with Gp2 and the fact they bind similar regions of RNA polymerase, we hypothesize that ε may serve a role in protection from phage infection.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Bacteriol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Bacteriol</journal-id>
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<title-group><article-title>ε, a New Subunit of RNA Polymerase Found in Gram-Positive Bacteria</article-title>
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<contrib-group><contrib contrib-type="author"><name><surname>Keller</surname>
<given-names>Andrew N.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
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<contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Xiao</given-names>
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<xref ref-type="aff" rid="aff1"><sup>a</sup>
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<contrib contrib-type="author"><name><surname>Wiedermannová</surname>
<given-names>Jana</given-names>
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<xref ref-type="aff" rid="aff2"><sup>b</sup>
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<contrib contrib-type="author"><name><surname>Delumeau</surname>
<given-names>Olivier</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
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<contrib contrib-type="author"><name><surname>Krásný</surname>
<given-names>Libor</given-names>
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<xref ref-type="aff" rid="aff2"><sup>b</sup>
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<contrib contrib-type="author" corresp="yes"><name><surname>Lewis</surname>
<given-names>Peter J.</given-names>
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<xref ref-type="aff" rid="aff1"><sup>a</sup>
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<aff id="aff1"><label>a</label>
School of Environmental and Life Sciences, University of Newcastle, Callghan, New South Wales, Australia</aff>
<aff id="aff2"><label>b</label>
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic</aff>
<aff id="aff3"><label>c</label>
INRA, UMR-1319 Micalis, Jouy-en-Josas, France</aff>
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<author-notes><corresp id="cor1">Address correspondence to Peter J. Lewis, <email>Peter.Lewis@newcastle.edu.au</email>
.</corresp>
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<pub-date pub-type="ppub"><month>10</month>
<year>2014</year>
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<volume>196</volume>
<issue>20</issue>
<fpage>3622</fpage>
<lpage>3632</lpage>
<history><date date-type="received"><day>23</day>
<month>6</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>31</day>
<month>7</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2014</copyright-year>
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<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zjb02014003622.pdf"></self-uri>
<abstract><p>RNA polymerase in bacteria is a multisubunit protein complex that is essential for gene expression. We have identified a new subunit of RNA polymerase present in the high-A+T <named-content content-type="genus-species">Firmicutes</named-content>
phylum of Gram-positive bacteria and have named it ε. Previously ε had been identified as a small protein (ω<sub>1</sub>
) that copurified with RNA polymerase. We have solved the structure of ε by X-ray crystallography and show that it is not an ω subunit. Rather, ε bears remarkable similarity to the Gp2 family of phage proteins involved in the inhibition of host cell transcription following infection. Deletion of ε shows no phenotype and has no effect on the transcriptional profile of the cell. Determination of the location of ε within the assembly of RNA polymerase core by single-particle analysis suggests that it binds toward the downstream side of the DNA binding cleft. Due to the structural similarity of ε with Gp2 and the fact they bind similar regions of RNA polymerase, we hypothesize that ε may serve a role in protection from phage infection.</p>
</abstract>
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