AustralieFrV1, Main, Exploration, bibRecordById, Pascal:06-0460322

Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

Identifieur interne : 00A050 ( Main/Exploration ); précédent : 00A049; suivant : 00A051

Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

Auteurs : Jean-Philippe Ral [France] ; Christophe Colleoni [France] ; Fabrice Wattebled [France] ; David Dauvillee [France] ; Clément Nempont [France] ; Philippe Deschamps [France] ; ZHONGYI LI [Australie] ; Matthew K. Morell [Australie] ; Ravindra Chibbar [Canada] ; Saul Purton [Royaume-Uni] ; Christophe D'Hulst [France] ; Steven G. Ball [France]

Source :

Plant physiology : (Bethesda) [ 0032-0889 ] ; 2006.

RBID : Pascal:06-0460322

Descripteurs français

Pascal (Inist)
- Amidon, Métabolisme, Pyrophosphorylase, Starch synthase, Mutation, Gène, Feuille végétal, Transcription, Chlamydomonas reinhardtii, Carbone dioxyde, Polyoside.
Wicri :
- topic : Amidon.

English descriptors

KwdEn :
- Carbon dioxide, Chlamydomonas reinhardtii, Gene, Metabolism, Mutation, Plant leaf, Polysaccharide, Pyrophosphorylase, Starch, Starch synthase, Transcription.

Abstract

Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO₂ as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557617

Affiliations:

Le document en format XML

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<author><name sortKey="Chibbar, Ravindra" sort="Chibbar, Ravindra" uniqKey="Chibbar R" first="Ravindra" last="Chibbar">Ravindra Chibbar</name>
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<s2>Saskatoon, Saskatchewan, S7N 0W9</s2>
<s3>CAN</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Saskatoon, Saskatchewan, S7N 0W9</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Purton, Saul" sort="Purton, Saul" uniqKey="Purton S" first="Saul" last="Purton">Saul Purton</name>
<affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Department of Biology, University College London</s1>
<s2>London WC1E 6BT</s2>
<s3>GBR</s3>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<placeName><settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
<orgName type="university">University College de Londres</orgName>
</affiliation>
</author>
<author><name sortKey="D Hulst, Christophe" sort="D Hulst, Christophe" uniqKey="D Hulst C" first="Christophe" last="D'Hulst">Christophe D'Hulst</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
<s2>59655 Villeneuve d'Ascq</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ball, Steven G" sort="Ball, Steven G" uniqKey="Ball S" first="Steven G." last="Ball">Steven G. Ball</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
<s2>59655 Villeneuve d'Ascq</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant physiology : (Bethesda)</title>
<title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title>
<idno type="ISSN">0032-0889</idno>
<imprint><date when="2006">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant physiology : (Bethesda)</title>
<title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title>
<idno type="ISSN">0032-0889</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon dioxide</term>
<term>Chlamydomonas reinhardtii</term>
<term>Gene</term>
<term>Metabolism</term>
<term>Mutation</term>
<term>Plant leaf</term>
<term>Polysaccharide</term>
<term>Pyrophosphorylase</term>
<term>Starch</term>
<term>Starch synthase</term>
<term>Transcription</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Amidon</term>
<term>Métabolisme</term>
<term>Pyrophosphorylase</term>
<term>Starch synthase</term>
<term>Mutation</term>
<term>Gène</term>
<term>Feuille végétal</term>
<term>Transcription</term>
<term>Chlamydomonas reinhardtii</term>
<term>Carbone dioxyde</term>
<term>Polyoside</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Amidon</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO<sub>2</sub>
 as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>Canada</li>
<li>France</li>
<li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Grand Londres</li>
<li>Hauts-de-France</li>
<li>Nord-Pas-de-Calais</li>
</region>
<settlement><li>Londres</li>
<li>Villeneuve d'Ascq</li>
</settlement>
<orgName><li>University College de Londres</li>
</orgName>
</list>
<tree><country name="France"><region name="Hauts-de-France"><name sortKey="Ral, Jean Philippe" sort="Ral, Jean Philippe" uniqKey="Ral J" first="Jean-Philippe" last="Ral">Jean-Philippe Ral</name>
</region>
<name sortKey="Ball, Steven G" sort="Ball, Steven G" uniqKey="Ball S" first="Steven G." last="Ball">Steven G. Ball</name>
<name sortKey="Colleoni, Christophe" sort="Colleoni, Christophe" uniqKey="Colleoni C" first="Christophe" last="Colleoni">Christophe Colleoni</name>
<name sortKey="D Hulst, Christophe" sort="D Hulst, Christophe" uniqKey="D Hulst C" first="Christophe" last="D'Hulst">Christophe D'Hulst</name>
<name sortKey="Dauvillee, David" sort="Dauvillee, David" uniqKey="Dauvillee D" first="David" last="Dauvillee">David Dauvillee</name>
<name sortKey="Deschamps, Philippe" sort="Deschamps, Philippe" uniqKey="Deschamps P" first="Philippe" last="Deschamps">Philippe Deschamps</name>
<name sortKey="Nempont, Clement" sort="Nempont, Clement" uniqKey="Nempont C" first="Clément" last="Nempont">Clément Nempont</name>
<name sortKey="Wattebled, Fabrice" sort="Wattebled, Fabrice" uniqKey="Wattebled F" first="Fabrice" last="Wattebled">Fabrice Wattebled</name>
</country>
<country name="Australie"><noRegion><name sortKey="Zhongyi Li" sort="Zhongyi Li" uniqKey="Zhongyi Li" last="Zhongyi Li">ZHONGYI LI</name>
</noRegion>
<name sortKey="Morell, Matthew K" sort="Morell, Matthew K" uniqKey="Morell M" first="Matthew K." last="Morell">Matthew K. Morell</name>
</country>
<country name="Canada"><noRegion><name sortKey="Chibbar, Ravindra" sort="Chibbar, Ravindra" uniqKey="Chibbar R" first="Ravindra" last="Chibbar">Ravindra Chibbar</name>
</noRegion>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Purton, Saul" sort="Purton, Saul" uniqKey="Purton S" first="Saul" last="Purton">Saul Purton</name>
</region>
</country>
</tree>
</affiliations>
</record>

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Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

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