AustralieFrV1, PascalFrancis, Corpus, bibRecord, 004137

Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

Identifieur interne : 004137 ( PascalFrancis/Corpus ); précédent : 004136; suivant : 004138

Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

Auteurs : Jean-Philippe Ral ; Christophe Colleoni ; Fabrice Wattebled ; David Dauvillee ; Clément Nempont ; Philippe Deschamps ; ZHONGYI LI ; Matthew K. Morell ; Ravindra Chibbar ; Saul Purton ; Christophe D'Hulst ; Steven G. Ball

Source :

Plant physiology : (Bethesda) [ 0032-0889 ] ; 2006.

RBID : Pascal:06-0460322

Descripteurs français

Pascal (Inist)
- Amidon, Métabolisme, Pyrophosphorylase, Starch synthase, Mutation, Gène, Feuille végétal, Transcription, Chlamydomonas reinhardtii, Carbone dioxyde, Polyoside.

English descriptors

KwdEn :
- Carbon dioxide, Chlamydomonas reinhardtii, Gene, Metabolism, Mutation, Plant leaf, Polysaccharide, Pyrophosphorylase, Starch, Starch synthase, Transcription.

Abstract

Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO₂ as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A14	`03`			`@1 Plant Biotechnology Institute, National Research Council of Canada @2 Saskatoon, Saskatchewan, S7N 0W9 @3 CAN @Z 9 aut.`
A14	`04`			`@1 Department of Biology, University College London @2 London WC1E 6BT @3 GBR @Z 10 aut.`
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C01	`01`		`ENG`	@0 Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO₂ as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.
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Format Inist (serveur)

NO :	PASCAL 06-0460322 INIST
ET :	Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii^[W][OA]
AU :	RAL (Jean-Philippe); COLLEONI (Christophe); WATTEBLED (Fabrice); DAUVILLEE (David); NEMPONT (Clément); DESCHAMPS (Philippe); ZHONGYI LI; MORELL (Matthew K.); CHIBBAR (Ravindra); PURTON (Saul); D'HULST (Christophe); BALL (Steven G.)
AF :	Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche/59655 Villeneuve d'Ascq/France (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 11 aut., 12 aut.); CSIRO Plant Industry, Australian Capital Territory 2601/Canberra/Australie (7 aut., 8 aut.); Plant Biotechnology Institute, National Research Council of Canada/Saskatoon, Saskatchewan, S7N 0W9/Canada (9 aut.); Department of Biology, University College London/London WC1E 6BT/Royaume-Uni (10 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant physiology : (Bethesda); ISSN 0032-0889; Coden PPHYA5; Etats-Unis; Da. 2006; Vol. 142; No. 1; Pp. 305-317; Bibl. 1 p.1/4
LA :	Anglais
EA :	Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO₂ as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.
CC :	002A10E02
FD :	Amidon; Métabolisme; Pyrophosphorylase; Starch synthase; Mutation; Gène; Feuille végétal; Transcription; Chlamydomonas reinhardtii; Carbone dioxyde; Polyoside
FG :	Transferases; Enzyme; Hexosyltransferases; Glycosyltransferases; Chlorophyceae; Chlorophyta; Algae; Thallophyta
ED :	Starch; Metabolism; Pyrophosphorylase; Starch synthase; Mutation; Gene; Plant leaf; Transcription; Chlamydomonas reinhardtii; Carbon dioxide; Polysaccharide
EG :	Transferases; Enzyme; Hexosyltransferases; Glycosyltransferases; Chlorophyceae; Chlorophyta; Algae; Thallophyta
SD :	Almidón; Metabolismo; Pyrophosphorylase; Starch synthase; Mutación; Gen; Hoja vegetal; Transcripción; Chlamydomonas reinhardtii; Carbono dióxido; Poliósido
LO :	INIST-3000.354000157136890260
ID :	06-0460322

Links to Exploration step

Pascal:06-0460322

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii<sup>[W][OA]</sup>
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<affiliation><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
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<author><name sortKey="Wattebled, Fabrice" sort="Wattebled, Fabrice" uniqKey="Wattebled F" first="Fabrice" last="Wattebled">Fabrice Wattebled</name>
<affiliation><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
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<author><name sortKey="Dauvillee, David" sort="Dauvillee, David" uniqKey="Dauvillee D" first="David" last="Dauvillee">David Dauvillee</name>
<affiliation><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
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<author><name sortKey="Nempont, Clement" sort="Nempont, Clement" uniqKey="Nempont C" first="Clément" last="Nempont">Clément Nempont</name>
<affiliation><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
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<affiliation><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
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<author><name sortKey="Zhongyi Li" sort="Zhongyi Li" uniqKey="Zhongyi Li" last="Zhongyi Li">ZHONGYI LI</name>
<affiliation><inist:fA14 i1="02"><s1>CSIRO Plant Industry, Australian Capital Territory 2601</s1>
<s2>Canberra</s2>
<s3>AUS</s3>
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<author><name sortKey="Morell, Matthew K" sort="Morell, Matthew K" uniqKey="Morell M" first="Matthew K." last="Morell">Matthew K. Morell</name>
<affiliation><inist:fA14 i1="02"><s1>CSIRO Plant Industry, Australian Capital Territory 2601</s1>
<s2>Canberra</s2>
<s3>AUS</s3>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
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</affiliation>
</author>
<author><name sortKey="Chibbar, Ravindra" sort="Chibbar, Ravindra" uniqKey="Chibbar R" first="Ravindra" last="Chibbar">Ravindra Chibbar</name>
<affiliation><inist:fA14 i1="03"><s1>Plant Biotechnology Institute, National Research Council of Canada</s1>
<s2>Saskatoon, Saskatchewan, S7N 0W9</s2>
<s3>CAN</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Purton, Saul" sort="Purton, Saul" uniqKey="Purton S" first="Saul" last="Purton">Saul Purton</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Biology, University College London</s1>
<s2>London WC1E 6BT</s2>
<s3>GBR</s3>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="D Hulst, Christophe" sort="D Hulst, Christophe" uniqKey="D Hulst C" first="Christophe" last="D'Hulst">Christophe D'Hulst</name>
<affiliation><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
<s2>59655 Villeneuve d'Ascq</s2>
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<sZ>11 aut.</sZ>
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</author>
<author><name sortKey="Ball, Steven G" sort="Ball, Steven G" uniqKey="Ball S" first="Steven G." last="Ball">Steven G. Ball</name>
<affiliation><inist:fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
<s2>59655 Villeneuve d'Ascq</s2>
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</author>
</analytic>
<series><title level="j" type="main">Plant physiology : (Bethesda)</title>
<title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title>
<idno type="ISSN">0032-0889</idno>
<imprint><date when="2006">2006</date>
</imprint>
</series>
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<seriesStmt><title level="j" type="main">Plant physiology : (Bethesda)</title>
<title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title>
<idno type="ISSN">0032-0889</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon dioxide</term>
<term>Chlamydomonas reinhardtii</term>
<term>Gene</term>
<term>Metabolism</term>
<term>Mutation</term>
<term>Plant leaf</term>
<term>Polysaccharide</term>
<term>Pyrophosphorylase</term>
<term>Starch</term>
<term>Starch synthase</term>
<term>Transcription</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Amidon</term>
<term>Métabolisme</term>
<term>Pyrophosphorylase</term>
<term>Starch synthase</term>
<term>Mutation</term>
<term>Gène</term>
<term>Feuille végétal</term>
<term>Transcription</term>
<term>Chlamydomonas reinhardtii</term>
<term>Carbone dioxyde</term>
<term>Polyoside</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en">Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO<sub>2</sub>
 as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.</div>
</front>
</TEI>
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<fA03 i2="1"><s0>Plant physiol. : (Bethesda)</s0>
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<fA05><s2>142</s2>
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<fA06><s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii<sup>[W][OA]</sup>
</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>RAL (Jean-Philippe)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>COLLEONI (Christophe)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>WATTEBLED (Fabrice)</s1>
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<fA11 i1="04" i2="1"><s1>DAUVILLEE (David)</s1>
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<fA11 i1="05" i2="1"><s1>NEMPONT (Clément)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>DESCHAMPS (Philippe)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>ZHONGYI LI</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>MORELL (Matthew K.)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>CHIBBAR (Ravindra)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>PURTON (Saul)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>D'HULST (Christophe)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>BALL (Steven G.)</s1>
</fA11>
<fA14 i1="01"><s1>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche</s1>
<s2>59655 Villeneuve d'Ascq</s2>
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<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>CSIRO Plant Industry, Australian Capital Territory 2601</s1>
<s2>Canberra</s2>
<s3>AUS</s3>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Plant Biotechnology Institute, National Research Council of Canada</s1>
<s2>Saskatoon, Saskatchewan, S7N 0W9</s2>
<s3>CAN</s3>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Biology, University College London</s1>
<s2>London WC1E 6BT</s2>
<s3>GBR</s3>
<sZ>10 aut.</sZ>
</fA14>
<fA20><s1>305-317</s1>
</fA20>
<fA21><s1>2006</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
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<s2>3000</s2>
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</fA43>
<fA44><s0>0000</s0>
<s1>© 2006 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>1 p.1/4</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>06-0460322</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
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<fA64 i1="01" i2="1"><s0>Plant physiology : (Bethesda)</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO<sub>2</sub>
 as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A10E02</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Amidon</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Starch</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Almidón</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Métabolisme</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Metabolism</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Metabolismo</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Pyrophosphorylase</s0>
<s2>FE</s2>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Pyrophosphorylase</s0>
<s2>FE</s2>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Pyrophosphorylase</s0>
<s2>FE</s2>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Starch synthase</s0>
<s2>FE</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Starch synthase</s0>
<s2>FE</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Starch synthase</s0>
<s2>FE</s2>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Mutation</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Mutation</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Mutación</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Gène</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Gene</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Gen</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Feuille végétal</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Plant leaf</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Hoja vegetal</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Transcription</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Transcription</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Transcripción</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Chlamydomonas reinhardtii</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Chlamydomonas reinhardtii</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Chlamydomonas reinhardtii</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Carbone dioxyde</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Carbon dioxide</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Carbono dióxido</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Polyoside</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Polysaccharide</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Poliósido</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Hexosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Hexosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Hexosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Chlorophyceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Chlorophyceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Chlorophyceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Chlorophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Chlorophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Chlorophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Algae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Algae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Algae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Thallophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Thallophyta</s0>
<s2>NS</s2>
</fC07>
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<ET>Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii<sup>[W][OA]</sup>
</ET>
<AU>RAL (Jean-Philippe); COLLEONI (Christophe); WATTEBLED (Fabrice); DAUVILLEE (David); NEMPONT (Clément); DESCHAMPS (Philippe); ZHONGYI LI; MORELL (Matthew K.); CHIBBAR (Ravindra); PURTON (Saul); D'HULST (Christophe); BALL (Steven G.)</AU>
<AF>Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique, Université des Sciences et Technologies de Lille, Institut Fédératif de Recherche/59655 Villeneuve d'Ascq/France (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 11 aut., 12 aut.); CSIRO Plant Industry, Australian Capital Territory 2601/Canberra/Australie (7 aut., 8 aut.); Plant Biotechnology Institute, National Research Council of Canada/Saskatoon, Saskatchewan, S7N 0W9/Canada (9 aut.); Department of Biology, University College London/London WC1E 6BT/Royaume-Uni (10 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant physiology : (Bethesda); ISSN 0032-0889; Coden PPHYA5; Etats-Unis; Da. 2006; Vol. 142; No. 1; Pp. 305-317; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the middle of the night phase if the algae are provided with acetate and CO<sub>2</sub>
 as a carbon source. We show that this rhythm is controlled by the circadian clock and is tightly correlated to ADP-glucose pyrophosphorylase activity. Persistence of this rhythm depends on the presence of either soluble starch synthase III or granule-bound starch synthase I (GBSSI). We show that both enzymes play a similar function in synthesizing the long glucan fraction that interconnects the amylopectin clusters. We demonstrate that in log phase-oscillating cultures, GBSSI is required to obtain maximal polysaccharide content and fully compensates for the loss of soluble starch synthase III. A point mutation in the GBSSI gene that prevents extension of amylopectin chains, but retains the enzyme's normal ability to extend maltooligosaccharides, abolishes the function of GBSSI both in amylopectin and amylose synthesis and leads to a decrease in starch content in oscillating cultures. We propose that GBSSI has evolved as a major enzyme of amylopectin synthesis and that amylose synthesis comes as a secondary consequence of prolonged synthesis by GBSSI in arrhythmic systems. Maintenance in higher plant leaves of circadian clock control of GBSSI transcription is discussed.</EA>
<CC>002A10E02</CC>
<FD>Amidon; Métabolisme; Pyrophosphorylase; Starch synthase; Mutation; Gène; Feuille végétal; Transcription; Chlamydomonas reinhardtii; Carbone dioxyde; Polyoside</FD>
<FG>Transferases; Enzyme; Hexosyltransferases; Glycosyltransferases; Chlorophyceae; Chlorophyta; Algae; Thallophyta</FG>
<ED>Starch; Metabolism; Pyrophosphorylase; Starch synthase; Mutation; Gene; Plant leaf; Transcription; Chlamydomonas reinhardtii; Carbon dioxide; Polysaccharide</ED>
<EG>Transferases; Enzyme; Hexosyltransferases; Glycosyltransferases; Chlorophyceae; Chlorophyta; Algae; Thallophyta</EG>
<SD>Almidón; Metabolismo; Pyrophosphorylase; Starch synthase; Mutación; Gen; Hoja vegetal; Transcripción; Chlamydomonas reinhardtii; Carbono dióxido; Poliósido</SD>
<LO>INIST-3000.354000157136890260</LO>
<ID>06-0460322</ID>
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Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

Circadian clock regulation of starch metabolism establishes GBSSI as a major contributor to amylopectin synthesis in Chlamydomonas reinhardtii[W][OA]

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