AustralieFrV1, Main, Exploration, bibRecordById, Pascal:05-0396673

Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains

Identifieur interne : 00A550 ( Main/Exploration ); précédent : 00A549; suivant : 00A551

Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains

Auteurs : Pascal Quenee [France] ; Elodie Lepage [France] ; Woojin Scott Kim [Australie] ; Gilles Vergnaud [France] ; Alexandra Gruss [France]

Source :

FEMS microbiology letters [ 0378-1097 ] ; 2005.

RBID : Pascal:05-0396673

Descripteurs français

Pascal (Inist)
- Lactococcus lactis, Polymorphisme, Diversité biologique, Marqueur RAPD, DNA microsatellite, Dendogramme.

English descriptors

KwdEn :
- Biodiversity, Dendogram, Lactococcus lactis, Microsatellite DNA, Polymorphism, Random amplified polymorphic DNA marker.

Abstract

Genome plasticity is considered as a means for bacteria to adapt to their environment. Plasticity in tandem repeat sequences on bacterial genomes has been recently exploited to trace the epidemiology of pathogens. Here, we examine the utility of minisatellite (i.e., a repeat unit of six nucleotides or more) typing in non-pathogenic food bacteria of the species Lactococcus lactis. Thirty-four minisatellites identified on the sequenced L. lactis ssp. lactis strain IL1403 genome were first analyzed in 10 closely related ssp. lactis strains, as determined by randomly amplified polymorphic DNA (RAPD). The selected tandem repeats varied in length, percent identity between repeats, and locations. We showed that: (i) the greatest polymorphism was in orfs encoding exported proteins or in intergenic regions; (ii) two thirds of minisatellites were little- or non-variable, despite as much as 90% identity between tandem repeats; and (iii) dendrograms based on either RAPD or minisatellite analyses were similar. Seven minisatellites identified in this study are potentially useful for lactococcal typing. We then asked whether tandem repeats in L. lactis were stable upon very long-term (up to two years) storage. Despite large rearrangements previously reported in derivative strains, just one of 10 minisatellites tested underwent an alteration, suggesting that tandem repeat rearrangements probably occur during active DNA replication. We conclude that multiple locus minisatellite analysis can be a valuable tool to follow lactococcal strain diversity.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Genome plasticity is considered as a means for bacteria to adapt to their environment. Plasticity in tandem repeat sequences on bacterial genomes has been recently exploited to trace the epidemiology of pathogens. Here, we examine the utility of minisatellite (i.e., a repeat unit of six nucleotides or more) typing in non-pathogenic food bacteria of the species Lactococcus lactis. Thirty-four minisatellites identified on the sequenced L. lactis ssp. lactis strain IL1403 genome were first analyzed in 10 closely related ssp. lactis strains, as determined by randomly amplified polymorphic DNA (RAPD). The selected tandem repeats varied in length, percent identity between repeats, and locations. We showed that: (i) the greatest polymorphism was in orfs encoding exported proteins or in intergenic regions; (ii) two thirds of minisatellites were little- or non-variable, despite as much as 90% identity between tandem repeats; and (iii) dendrograms based on either RAPD or minisatellite analyses were similar. Seven minisatellites identified in this study are potentially useful for lactococcal typing. We then asked whether tandem repeats in L. lactis were stable upon very long-term (up to two years) storage. Despite large rearrangements previously reported in derivative strains, just one of 10 minisatellites tested underwent an alteration, suggesting that tandem repeat rearrangements probably occur during active DNA replication. We conclude that multiple locus minisatellite analysis can be a valuable tool to follow lactococcal strain diversity.</div>
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Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains

Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri