Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains
Identifieur interne : 00B157 ( Main/Merge ); précédent : 00B156; suivant : 00B158Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains
Auteurs : Pascal Quenee [France] ; Elodie Lepage [France] ; Woojin Scott Kim [Australie] ; Gilles Vergnaud [France] ; Alexandra Gruss [France]Source :
- FEMS microbiology letters [ 0378-1097 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Genome plasticity is considered as a means for bacteria to adapt to their environment. Plasticity in tandem repeat sequences on bacterial genomes has been recently exploited to trace the epidemiology of pathogens. Here, we examine the utility of minisatellite (i.e., a repeat unit of six nucleotides or more) typing in non-pathogenic food bacteria of the species Lactococcus lactis. Thirty-four minisatellites identified on the sequenced L. lactis ssp. lactis strain IL1403 genome were first analyzed in 10 closely related ssp. lactis strains, as determined by randomly amplified polymorphic DNA (RAPD). The selected tandem repeats varied in length, percent identity between repeats, and locations. We showed that: (i) the greatest polymorphism was in orfs encoding exported proteins or in intergenic regions; (ii) two thirds of minisatellites were little- or non-variable, despite as much as 90% identity between tandem repeats; and (iii) dendrograms based on either RAPD or minisatellite analyses were similar. Seven minisatellites identified in this study are potentially useful for lactococcal typing. We then asked whether tandem repeats in L. lactis were stable upon very long-term (up to two years) storage. Despite large rearrangements previously reported in derivative strains, just one of 10 minisatellites tested underwent an alteration, suggesting that tandem repeat rearrangements probably occur during active DNA replication. We conclude that multiple locus minisatellite analysis can be a valuable tool to follow lactococcal strain diversity.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 004782
- to stream PascalFrancis, to step Curation: 001921
- to stream PascalFrancis, to step Checkpoint: 004507
Links to Exploration step
Pascal:05-0396673Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains</title>
<author><name sortKey="Quenee, Pascal" sort="Quenee, Pascal" uniqKey="Quenee P" first="Pascal" last="Quenee">Pascal Quenee</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Recherches Laitières et Génétique Appliquée - URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert</s1>
<s2>78352 Jouy en Josas</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Jouy en Josas</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lepage, Elodie" sort="Lepage, Elodie" uniqKey="Lepage E" first="Elodie" last="Lepage">Elodie Lepage</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Recherches Laitières et Génétique Appliquée - URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert</s1>
<s2>78352 Jouy en Josas</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Jouy en Josas</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Woojin Scott" sort="Kim, Woojin Scott" uniqKey="Kim W" first="Woojin Scott" last="Kim">Woojin Scott Kim</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>School of Biotechnology and Biomolecular Sciences, University of New South Wales</s1>
<s2>Sydney, NSW 2052</s2>
<s3>AUS</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Sydney, NSW 2052</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vergnaud, Gilles" sort="Vergnaud, Gilles" uniqKey="Vergnaud G" first="Gilles" last="Vergnaud">Gilles Vergnaud</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Laboratoire GPMS, Institut de Génétique et Microbiologie, Bat 400, Universite Paris-Sud</s1>
<s2>91405 Orsay</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>91405 Orsay</wicri:noRegion>
<wicri:noRegion>Universite Paris-Sud</wicri:noRegion>
<placeName><settlement type="city">Orsay</settlement>
<region type="region" nuts="2">Île-de-France</region>
</placeName>
<orgName type="university">Université Paris-Sud</orgName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Division d'Analyses Microbiologiques, Centre dEtudes du Bouchet BP3</s1>
<s2>91710 Vert le Petit</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Vert le Petit</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gruss, Alexandra" sort="Gruss, Alexandra" uniqKey="Gruss A" first="Alexandra" last="Gruss">Alexandra Gruss</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Recherches Laitières et Génétique Appliquée - URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert</s1>
<s2>78352 Jouy en Josas</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Jouy en Josas</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">05-0396673</idno>
<date when="2005">2005</date>
<idno type="stanalyst">PASCAL 05-0396673 LGMI</idno>
<idno type="RBID">Pascal:05-0396673</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">004782</idno>
<idno type="wicri:Area/PascalFrancis/Curation">001921</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">004507</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">004507</idno>
<idno type="wicri:doubleKey">0378-1097:2005:Quenee P:minisatellite:polymorphism:as</idno>
<idno type="wicri:Area/Main/Merge">00B157</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains</title>
<author><name sortKey="Quenee, Pascal" sort="Quenee, Pascal" uniqKey="Quenee P" first="Pascal" last="Quenee">Pascal Quenee</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Recherches Laitières et Génétique Appliquée - URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert</s1>
<s2>78352 Jouy en Josas</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Jouy en Josas</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lepage, Elodie" sort="Lepage, Elodie" uniqKey="Lepage E" first="Elodie" last="Lepage">Elodie Lepage</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Recherches Laitières et Génétique Appliquée - URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert</s1>
<s2>78352 Jouy en Josas</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Jouy en Josas</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Woojin Scott" sort="Kim, Woojin Scott" uniqKey="Kim W" first="Woojin Scott" last="Kim">Woojin Scott Kim</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>School of Biotechnology and Biomolecular Sciences, University of New South Wales</s1>
<s2>Sydney, NSW 2052</s2>
<s3>AUS</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Sydney, NSW 2052</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vergnaud, Gilles" sort="Vergnaud, Gilles" uniqKey="Vergnaud G" first="Gilles" last="Vergnaud">Gilles Vergnaud</name>
<affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Laboratoire GPMS, Institut de Génétique et Microbiologie, Bat 400, Universite Paris-Sud</s1>
<s2>91405 Orsay</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>91405 Orsay</wicri:noRegion>
<wicri:noRegion>Universite Paris-Sud</wicri:noRegion>
<placeName><settlement type="city">Orsay</settlement>
<region type="region" nuts="2">Île-de-France</region>
</placeName>
<orgName type="university">Université Paris-Sud</orgName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Division d'Analyses Microbiologiques, Centre dEtudes du Bouchet BP3</s1>
<s2>91710 Vert le Petit</s2>
<s3>FRA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Vert le Petit</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gruss, Alexandra" sort="Gruss, Alexandra" uniqKey="Gruss A" first="Alexandra" last="Gruss">Alexandra Gruss</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Recherches Laitières et Génétique Appliquée - URLGA, Institut National de la Recherche Agronomique, Domaine de Vilvert</s1>
<s2>78352 Jouy en Josas</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Jouy en Josas</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">FEMS microbiology letters</title>
<title level="j" type="abbreviated">FEMS microbiol. lett.</title>
<idno type="ISSN">0378-1097</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">FEMS microbiology letters</title>
<title level="j" type="abbreviated">FEMS microbiol. lett.</title>
<idno type="ISSN">0378-1097</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodiversity</term>
<term>Dendogram</term>
<term>Lactococcus lactis</term>
<term>Microsatellite DNA</term>
<term>Polymorphism</term>
<term>Random amplified polymorphic DNA marker</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Lactococcus lactis</term>
<term>Polymorphisme</term>
<term>Diversité biologique</term>
<term>Marqueur RAPD</term>
<term>DNA microsatellite</term>
<term>Dendogramme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Genome plasticity is considered as a means for bacteria to adapt to their environment. Plasticity in tandem repeat sequences on bacterial genomes has been recently exploited to trace the epidemiology of pathogens. Here, we examine the utility of minisatellite (i.e., a repeat unit of six nucleotides or more) typing in non-pathogenic food bacteria of the species Lactococcus lactis. Thirty-four minisatellites identified on the sequenced L. lactis ssp. lactis strain IL1403 genome were first analyzed in 10 closely related ssp. lactis strains, as determined by randomly amplified polymorphic DNA (RAPD). The selected tandem repeats varied in length, percent identity between repeats, and locations. We showed that: (i) the greatest polymorphism was in orfs encoding exported proteins or in intergenic regions; (ii) two thirds of minisatellites were little- or non-variable, despite as much as 90% identity between tandem repeats; and (iii) dendrograms based on either RAPD or minisatellite analyses were similar. Seven minisatellites identified in this study are potentially useful for lactococcal typing. We then asked whether tandem repeats in L. lactis were stable upon very long-term (up to two years) storage. Despite large rearrangements previously reported in derivative strains, just one of 10 minisatellites tested underwent an alteration, suggesting that tandem repeat rearrangements probably occur during active DNA replication. We conclude that multiple locus minisatellite analysis can be a valuable tool to follow lactococcal strain diversity.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
</country>
<region><li>Île-de-France</li>
</region>
<settlement><li>Jouy en Josas</li>
<li>Orsay</li>
<li>Vert le Petit</li>
</settlement>
<orgName><li>Université Paris-Sud</li>
</orgName>
</list>
<tree><country name="France"><region name="Île-de-France"><name sortKey="Quenee, Pascal" sort="Quenee, Pascal" uniqKey="Quenee P" first="Pascal" last="Quenee">Pascal Quenee</name>
</region>
<name sortKey="Gruss, Alexandra" sort="Gruss, Alexandra" uniqKey="Gruss A" first="Alexandra" last="Gruss">Alexandra Gruss</name>
<name sortKey="Lepage, Elodie" sort="Lepage, Elodie" uniqKey="Lepage E" first="Elodie" last="Lepage">Elodie Lepage</name>
<name sortKey="Vergnaud, Gilles" sort="Vergnaud, Gilles" uniqKey="Vergnaud G" first="Gilles" last="Vergnaud">Gilles Vergnaud</name>
<name sortKey="Vergnaud, Gilles" sort="Vergnaud, Gilles" uniqKey="Vergnaud G" first="Gilles" last="Vergnaud">Gilles Vergnaud</name>
</country>
<country name="Australie"><noRegion><name sortKey="Kim, Woojin Scott" sort="Kim, Woojin Scott" uniqKey="Kim W" first="Woojin Scott" last="Kim">Woojin Scott Kim</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00B157 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 00B157 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Main |étape= Merge |type= RBID |clé= Pascal:05-0396673 |texte= Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |