Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants
Identifieur interne : 004D96 ( Main/Exploration ); précédent : 004D95; suivant : 004D97Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants
Auteurs : J. Sellares [États-Unis, Espagne] ; J. Reeve [États-Unis, Canada] ; A. Loupy [France] ; M. Mengel [États-Unis, Canada] ; B. Sis [Canada] ; A. Skene [États-Unis, Australie] ; D. G. De Freitas [Royaume-Uni] ; C. Kreepala [États-Unis, Canada] ; L. G. Hidalgo [États-Unis, Canada] ; K. S. Famulski [États-Unis, Canada] ; P. F. Halloran [États-Unis, Canada]Source :
- American journal of transplantation : (Print) [ 1600-6135 ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
Antibody-mediated rejection is the major cause of kidney transplant failure, but the histology-based diagnostic system misses most cases due to its requirement for C4d positivity. We hypothesized that gene expression data could be used to test biopsies for the presence of antibody-mediated rejection. To develop a molecular test, we prospectively assigned diagnoses, including C4d-negative antibody-mediated rejection, to 403 indication biopsies from 315 patients, based on histology (microcirculation lesions) and donor-specific HLA antibody. We then used microarray data to develop classifiers that assigned antibody-mediated rejection scores to each biopsy. The transcripts distinguishing antibody-mediated rejection from other conditions were mostly expressed in endothelial cells or NK cells, or were IFNG-inducible. The scores correlated with the presence of microcirculation lesions and donor-specific antibody. Of 45 biopsies with scores >0.5, 39 had been diagnosed as antibody-mediated rejection on the basis of histology and donor-specific antibody. High scores were also associated with unanimity among pathologists that antibody-mediated rejection was present. The molecular score also strongly predicted future graft loss in Cox regression analysis. We conclude that microarray assessment of gene expression can assign a probability of ABMR to transplant biopsies without knowledge of HLA antibody status, histology, or C4d staining, and predicts future failure.
Affiliations:
- Australie, Canada, Espagne, France, Royaume-Uni, États-Unis
- Angleterre, Catalogne, Grand Manchester, Île-de-France
- Barcelone, Manchester, Paris
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000A14
- to stream PascalFrancis, to step Curation: 005461
- to stream PascalFrancis, to step Checkpoint: 000781
- to stream Main, to step Merge: 004F55
- to stream Main, to step Curation: 004D96
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants</title>
<author><name sortKey="Sellares, J" sort="Sellares, J" uniqKey="Sellares J" first="J." last="Sellares">J. Sellares</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Servei de Nefrologia, Hospital de la Vall d'Hebron</s1>
<s2>Barcelona</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Reeve, J" sort="Reeve, J" uniqKey="Reeve J" first="J." last="Reeve">J. Reeve</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Loupy, A" sort="Loupy, A" uniqKey="Loupy A" first="A." last="Loupy">A. Loupy</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Kidney Transplant Department, Necker Hospital</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mengel, M" sort="Mengel, M" uniqKey="Mengel M" first="M." last="Mengel">M. Mengel</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sis, B" sort="Sis, B" uniqKey="Sis B" first="B." last="Sis">B. Sis</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Skene, A" sort="Skene, A" uniqKey="Skene A" first="A." last="Skene">A. Skene</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Anatomical Pathology, Austin Hospital</s1>
<s2>Heidelberg, Victoria</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Heidelberg, Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="De Freitas, D G" sort="De Freitas, D G" uniqKey="De Freitas D" first="D. G." last="De Freitas">D. G. De Freitas</name>
<affiliation wicri:level="3"><inist:fA14 i1="06"><s1>Department of Renal Medicine, Manchester Royal Infirmary</s1>
<s2>Manchester</s2>
<s3>GBR</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<placeName><settlement type="city">Manchester</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Manchester</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kreepala, C" sort="Kreepala, C" uniqKey="Kreepala C" first="C." last="Kreepala">C. Kreepala</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hidalgo, L G" sort="Hidalgo, L G" uniqKey="Hidalgo L" first="L. G." last="Hidalgo">L. G. Hidalgo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Famulski, K S" sort="Famulski, K S" uniqKey="Famulski K" first="K. S." last="Famulski">K. S. Famulski</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Halloran, P F" sort="Halloran, P F" uniqKey="Halloran P" first="P. F." last="Halloran">P. F. Halloran</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">13-0206839</idno>
<date when="2013">2013</date>
<idno type="stanalyst">PASCAL 13-0206839 INIST</idno>
<idno type="RBID">Pascal:13-0206839</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000A14</idno>
<idno type="wicri:Area/PascalFrancis/Curation">005461</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000781</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000781</idno>
<idno type="wicri:doubleKey">1600-6135:2013:Sellares J:molecular:diagnosis:of</idno>
<idno type="wicri:Area/Main/Merge">004F55</idno>
<idno type="wicri:Area/Main/Curation">004D96</idno>
<idno type="wicri:Area/Main/Exploration">004D96</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants</title>
<author><name sortKey="Sellares, J" sort="Sellares, J" uniqKey="Sellares J" first="J." last="Sellares">J. Sellares</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Servei de Nefrologia, Hospital de la Vall d'Hebron</s1>
<s2>Barcelona</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Reeve, J" sort="Reeve, J" uniqKey="Reeve J" first="J." last="Reeve">J. Reeve</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Loupy, A" sort="Loupy, A" uniqKey="Loupy A" first="A." last="Loupy">A. Loupy</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Kidney Transplant Department, Necker Hospital</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mengel, M" sort="Mengel, M" uniqKey="Mengel M" first="M." last="Mengel">M. Mengel</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sis, B" sort="Sis, B" uniqKey="Sis B" first="B." last="Sis">B. Sis</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Skene, A" sort="Skene, A" uniqKey="Skene A" first="A." last="Skene">A. Skene</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Anatomical Pathology, Austin Hospital</s1>
<s2>Heidelberg, Victoria</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Heidelberg, Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="De Freitas, D G" sort="De Freitas, D G" uniqKey="De Freitas D" first="D. G." last="De Freitas">D. G. De Freitas</name>
<affiliation wicri:level="3"><inist:fA14 i1="06"><s1>Department of Renal Medicine, Manchester Royal Infirmary</s1>
<s2>Manchester</s2>
<s3>GBR</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<placeName><settlement type="city">Manchester</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Manchester</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kreepala, C" sort="Kreepala, C" uniqKey="Kreepala C" first="C." last="Kreepala">C. Kreepala</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hidalgo, L G" sort="Hidalgo, L G" uniqKey="Hidalgo L" first="L. G." last="Hidalgo">L. G. Hidalgo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Famulski, K S" sort="Famulski, K S" uniqKey="Famulski K" first="K. S." last="Famulski">K. S. Famulski</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Halloran, P F" sort="Halloran, P F" uniqKey="Halloran P" first="P. F." last="Halloran">P. F. Halloran</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Alberta Transplant Applied Genomics Centre</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Edmonton, AB</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">American journal of transplantation : (Print)</title>
<title level="j" type="abbreviated">Am. j. transplant. : (Print)</title>
<idno type="ISSN">1600-6135</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">American journal of transplantation : (Print)</title>
<title level="j" type="abbreviated">Am. j. transplant. : (Print)</title>
<idno type="ISSN">1600-6135</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term>
<term>Graft(material)</term>
<term>Homotransplantation</term>
<term>Human</term>
<term>Kidney</term>
<term>Molecular biology</term>
<term>Molecular diagnostic</term>
<term>Rejection</term>
<term>Treatment</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Biologie moléculaire</term>
<term>Anticorps</term>
<term>Rejet</term>
<term>Homotransplantation</term>
<term>Greffon</term>
<term>Homme</term>
<term>Rein</term>
<term>Traitement</term>
<term>Diagnostic moléculaire</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Antibody-mediated rejection is the major cause of kidney transplant failure, but the histology-based diagnostic system misses most cases due to its requirement for C4d positivity. We hypothesized that gene expression data could be used to test biopsies for the presence of antibody-mediated rejection. To develop a molecular test, we prospectively assigned diagnoses, including C4d-negative antibody-mediated rejection, to 403 indication biopsies from 315 patients, based on histology (microcirculation lesions) and donor-specific HLA antibody. We then used microarray data to develop classifiers that assigned antibody-mediated rejection scores to each biopsy. The transcripts distinguishing antibody-mediated rejection from other conditions were mostly expressed in endothelial cells or NK cells, or were IFNG-inducible. The scores correlated with the presence of microcirculation lesions and donor-specific antibody. Of 45 biopsies with scores >0.5, 39 had been diagnosed as antibody-mediated rejection on the basis of histology and donor-specific antibody. High scores were also associated with unanimity among pathologists that antibody-mediated rejection was present. The molecular score also strongly predicted future graft loss in Cox regression analysis. We conclude that microarray assessment of gene expression can assign a probability of ABMR to transplant biopsies without knowledge of HLA antibody status, histology, or C4d staining, and predicts future failure.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>Canada</li>
<li>Espagne</li>
<li>France</li>
<li>Royaume-Uni</li>
<li>États-Unis</li>
</country>
<region><li>Angleterre</li>
<li>Catalogne</li>
<li>Grand Manchester</li>
<li>Île-de-France</li>
</region>
<settlement><li>Barcelone</li>
<li>Manchester</li>
<li>Paris</li>
</settlement>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Sellares, J" sort="Sellares, J" uniqKey="Sellares J" first="J." last="Sellares">J. Sellares</name>
</noRegion>
<name sortKey="Famulski, K S" sort="Famulski, K S" uniqKey="Famulski K" first="K. S." last="Famulski">K. S. Famulski</name>
<name sortKey="Halloran, P F" sort="Halloran, P F" uniqKey="Halloran P" first="P. F." last="Halloran">P. F. Halloran</name>
<name sortKey="Hidalgo, L G" sort="Hidalgo, L G" uniqKey="Hidalgo L" first="L. G." last="Hidalgo">L. G. Hidalgo</name>
<name sortKey="Kreepala, C" sort="Kreepala, C" uniqKey="Kreepala C" first="C." last="Kreepala">C. Kreepala</name>
<name sortKey="Mengel, M" sort="Mengel, M" uniqKey="Mengel M" first="M." last="Mengel">M. Mengel</name>
<name sortKey="Reeve, J" sort="Reeve, J" uniqKey="Reeve J" first="J." last="Reeve">J. Reeve</name>
<name sortKey="Skene, A" sort="Skene, A" uniqKey="Skene A" first="A." last="Skene">A. Skene</name>
</country>
<country name="Espagne"><region name="Catalogne"><name sortKey="Sellares, J" sort="Sellares, J" uniqKey="Sellares J" first="J." last="Sellares">J. Sellares</name>
</region>
</country>
<country name="Canada"><noRegion><name sortKey="Reeve, J" sort="Reeve, J" uniqKey="Reeve J" first="J." last="Reeve">J. Reeve</name>
</noRegion>
<name sortKey="Famulski, K S" sort="Famulski, K S" uniqKey="Famulski K" first="K. S." last="Famulski">K. S. Famulski</name>
<name sortKey="Halloran, P F" sort="Halloran, P F" uniqKey="Halloran P" first="P. F." last="Halloran">P. F. Halloran</name>
<name sortKey="Hidalgo, L G" sort="Hidalgo, L G" uniqKey="Hidalgo L" first="L. G." last="Hidalgo">L. G. Hidalgo</name>
<name sortKey="Kreepala, C" sort="Kreepala, C" uniqKey="Kreepala C" first="C." last="Kreepala">C. Kreepala</name>
<name sortKey="Mengel, M" sort="Mengel, M" uniqKey="Mengel M" first="M." last="Mengel">M. Mengel</name>
<name sortKey="Sis, B" sort="Sis, B" uniqKey="Sis B" first="B." last="Sis">B. Sis</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Loupy, A" sort="Loupy, A" uniqKey="Loupy A" first="A." last="Loupy">A. Loupy</name>
</region>
</country>
<country name="Australie"><noRegion><name sortKey="Skene, A" sort="Skene, A" uniqKey="Skene A" first="A." last="Skene">A. Skene</name>
</noRegion>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="De Freitas, D G" sort="De Freitas, D G" uniqKey="De Freitas D" first="D. G." last="De Freitas">D. G. De Freitas</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004D96 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004D96 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Main |étape= Exploration |type= RBID |clé= Pascal:13-0206839 |texte= Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |