AustralieFrV1, PascalFrancis, Curation, bibRecord, 005461

Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants

Identifieur interne : 005461 ( PascalFrancis/Curation ); précédent : 005460; suivant : 005462

Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants

Auteurs : J. Sellares [États-Unis, Espagne] ; J. Reeve [États-Unis, Canada] ; A. Loupy [France] ; M. Mengel [États-Unis, Canada] ; B. Sis [Canada] ; A. Skene [États-Unis, Australie] ; D. G. De Freitas [Royaume-Uni] ; C. Kreepala [États-Unis, Canada] ; L. G. Hidalgo [États-Unis, Canada] ; K. S. Famulski [États-Unis, Canada] ; P. F. Halloran [États-Unis, Canada]

Source :

American journal of transplantation : (Print) [ 1600-6135 ] ; 2013.

RBID : Pascal:13-0206839

Descripteurs français

Pascal (Inist)
- Biologie moléculaire, Anticorps, Rejet, Homotransplantation, Greffon, Homme, Rein, Traitement, Diagnostic moléculaire.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Antibody, Graft(material), Homotransplantation, Human, Kidney, Molecular biology, Molecular diagnostic, Rejection, Treatment.

Abstract

Antibody-mediated rejection is the major cause of kidney transplant failure, but the histology-based diagnostic system misses most cases due to its requirement for C4d positivity. We hypothesized that gene expression data could be used to test biopsies for the presence of antibody-mediated rejection. To develop a molecular test, we prospectively assigned diagnoses, including C4d-negative antibody-mediated rejection, to 403 indication biopsies from 315 patients, based on histology (microcirculation lesions) and donor-specific HLA antibody. We then used microarray data to develop classifiers that assigned antibody-mediated rejection scores to each biopsy. The transcripts distinguishing antibody-mediated rejection from other conditions were mostly expressed in endothelial cells or NK cells, or were IFNG-inducible. The scores correlated with the presence of microcirculation lesions and donor-specific antibody. Of 45 biopsies with scores >0.5, 39 had been diagnosed as antibody-mediated rejection on the basis of histology and donor-specific antibody. High scores were also associated with unanimity among pathologists that antibody-mediated rejection was present. The molecular score also strongly predicted future graft loss in Cox regression analysis. We conclude that microarray assessment of gene expression can assign a probability of ABMR to transplant biopsies without knowledge of HLA antibody status, histology, or C4d staining, and predicts future failure.

A01	`01`	`1`		`@0 1600-6135`
A03		`1`		`@0 Am. j. transplant. : (Print)`
A05				`@2 13`
A06				`@2 4`
A08	`01`	`1`	`ENG`	`@1 Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants`
A11	`01`	`1`		`@1 SELLARES (J.)`
A11	`02`	`1`		`@1 REEVE (J.)`
A11	`03`	`1`		`@1 LOUPY (A.)`
A11	`04`	`1`		`@1 MENGEL (M.)`
A11	`05`	`1`		`@1 SIS (B.)`
A11	`06`	`1`		`@1 SKENE (A.)`
A11	`07`	`1`		`@1 DE FREITAS (D. G.)`
A11	`08`	`1`		`@1 KREEPALA (C.)`
A11	`09`	`1`		`@1 HIDALGO (L. G.)`
A11	`10`	`1`		`@1 FAMULSKI (K. S.)`
A11	`11`	`1`		`@1 HALLORAN (P. F.)`
A14	`01`			`@1 Alberta Transplant Applied Genomics Centre @2 Edmonton, Alberta @3 USA @Z 1 aut. @Z 2 aut. @Z 4 aut. @Z 6 aut. @Z 8 aut. @Z 9 aut. @Z 10 aut. @Z 11 aut.`
A14	`02`			`@1 Servei de Nefrologia, Hospital de la Vall d'Hebron @2 Barcelona @3 ESP @Z 1 aut.`
A14	`03`			`@1 Department of Laboratory Medicine and Pathology, University of Alberta @2 Edmonton, AB @3 CAN @Z 2 aut. @Z 4 aut. @Z 5 aut. @Z 9 aut. @Z 10 aut.`
A14	`04`			`@1 Kidney Transplant Department, Necker Hospital @2 Paris @3 FRA @Z 3 aut.`
A14	`05`			`@1 Department of Anatomical Pathology, Austin Hospital @2 Heidelberg, Victoria @3 AUS @Z 6 aut.`
A14	`06`			`@1 Department of Renal Medicine, Manchester Royal Infirmary @2 Manchester @3 GBR @Z 7 aut.`
A14	`07`			`@1 Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta @2 Edmonton, AB @3 CAN @Z 8 aut. @Z 11 aut.`
A20				`@1 971-983`
A21				`@1 2013`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 27587 @5 354000504184910190`
A44				`@0 0000 @1 © 2013 INIST-CNRS. All rights reserved.`
A45				`@0 63 ref.`
A47	`01`	`1`		`@0 13-0206839`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 American journal of transplantation : (Print)`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Antibody-mediated rejection is the major cause of kidney transplant failure, but the histology-based diagnostic system misses most cases due to its requirement for C4d positivity. We hypothesized that gene expression data could be used to test biopsies for the presence of antibody-mediated rejection. To develop a molecular test, we prospectively assigned diagnoses, including C4d-negative antibody-mediated rejection, to 403 indication biopsies from 315 patients, based on histology (microcirculation lesions) and donor-specific HLA antibody. We then used microarray data to develop classifiers that assigned antibody-mediated rejection scores to each biopsy. The transcripts distinguishing antibody-mediated rejection from other conditions were mostly expressed in endothelial cells or NK cells, or were IFNG-inducible. The scores correlated with the presence of microcirculation lesions and donor-specific antibody. Of 45 biopsies with scores >0.5, 39 had been diagnosed as antibody-mediated rejection on the basis of histology and donor-specific antibody. High scores were also associated with unanimity among pathologists that antibody-mediated rejection was present. The molecular score also strongly predicted future graft loss in Cox regression analysis. We conclude that microarray assessment of gene expression can assign a probability of ABMR to transplant biopsies without knowledge of HLA antibody status, histology, or C4d staining, and predicts future failure.
C02	`01`	`X`		`@0 002B25`
C03	`01`	`X`	`FRE`	`@0 Biologie moléculaire @5 01`
C03	`01`	`X`	`ENG`	`@0 Molecular biology @5 01`
C03	`01`	`X`	`SPA`	`@0 Biología molecular @5 01`
C03	`02`	`X`	`FRE`	`@0 Anticorps @5 02`
C03	`02`	`X`	`ENG`	`@0 Antibody @5 02`
C03	`02`	`X`	`SPA`	`@0 Anticuerpo @5 02`
C03	`03`	`X`	`FRE`	`@0 Rejet @5 03`
C03	`03`	`X`	`ENG`	`@0 Rejection @5 03`
C03	`03`	`X`	`SPA`	`@0 Rechazo @5 03`
C03	`04`	`X`	`FRE`	`@0 Homotransplantation @5 04`
C03	`04`	`X`	`ENG`	`@0 Homotransplantation @5 04`
C03	`04`	`X`	`SPA`	`@0 Homotrasplante @5 04`
C03	`05`	`X`	`FRE`	`@0 Greffon @5 05`
C03	`05`	`X`	`ENG`	`@0 Graft(material) @5 05`
C03	`05`	`X`	`SPA`	`@0 Injerto(material) @5 05`
C03	`06`	`X`	`FRE`	`@0 Homme @5 06`
C03	`06`	`X`	`ENG`	`@0 Human @5 06`
C03	`06`	`X`	`SPA`	`@0 Hombre @5 06`
C03	`07`	`X`	`FRE`	`@0 Rein @5 08`
C03	`07`	`X`	`ENG`	`@0 Kidney @5 08`
C03	`07`	`X`	`SPA`	`@0 Riñón @5 08`
C03	`08`	`X`	`FRE`	`@0 Traitement @5 25`
C03	`08`	`X`	`ENG`	`@0 Treatment @5 25`
C03	`08`	`X`	`SPA`	`@0 Tratamiento @5 25`
C03	`09`	`X`	`FRE`	`@0 Diagnostic moléculaire @4 CD @5 96`
C03	`09`	`X`	`ENG`	`@0 Molecular diagnostic @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Chirurgie @5 37`
C07	`01`	`X`	`ENG`	`@0 Surgery @5 37`
C07	`01`	`X`	`SPA`	`@0 Cirugía @5 37`
C07	`02`	`X`	`FRE`	`@0 Greffe @5 38`
C07	`02`	`X`	`ENG`	`@0 Graft @5 38`
C07	`02`	`X`	`SPA`	`@0 Injerto @5 38`
C07	`03`	`X`	`FRE`	`@0 Transplantation @5 39`
C07	`03`	`X`	`ENG`	`@0 Transplantation @5 39`
C07	`03`	`X`	`SPA`	`@0 Trasplantación @5 39`
C07	`04`	`X`	`FRE`	`@0 Appareil urinaire @5 40`
C07	`04`	`X`	`ENG`	`@0 Urinary system @5 40`
C07	`04`	`X`	`SPA`	`@0 Aparato urinario @5 40`
N21				`@1 189`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A14

Links to Exploration step

Pascal:13-0206839

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants</title>
<author><name sortKey="Sellares, J" sort="Sellares, J" uniqKey="Sellares J" first="J." last="Sellares">J. Sellares</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Servei de Nefrologia, Hospital de la Vall d'Hebron</s1>
<s2>Barcelona</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
</affiliation>
</author>
<author><name sortKey="Reeve, J" sort="Reeve, J" uniqKey="Reeve J" first="J." last="Reeve">J. Reeve</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Loupy, A" sort="Loupy, A" uniqKey="Loupy A" first="A." last="Loupy">A. Loupy</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Kidney Transplant Department, Necker Hospital</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Mengel, M" sort="Mengel, M" uniqKey="Mengel M" first="M." last="Mengel">M. Mengel</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Sis, B" sort="Sis, B" uniqKey="Sis B" first="B." last="Sis">B. Sis</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Skene, A" sort="Skene, A" uniqKey="Skene A" first="A." last="Skene">A. Skene</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Anatomical Pathology, Austin Hospital</s1>
<s2>Heidelberg, Victoria</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
</affiliation>
</author>
<author><name sortKey="De Freitas, D G" sort="De Freitas, D G" uniqKey="De Freitas D" first="D. G." last="De Freitas">D. G. De Freitas</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Renal Medicine, Manchester Royal Infirmary</s1>
<s2>Manchester</s2>
<s3>GBR</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
</affiliation>
</author>
<author><name sortKey="Kreepala, C" sort="Kreepala, C" uniqKey="Kreepala C" first="C." last="Kreepala">C. Kreepala</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Hidalgo, L G" sort="Hidalgo, L G" uniqKey="Hidalgo L" first="L. G." last="Hidalgo">L. G. Hidalgo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Famulski, K S" sort="Famulski, K S" uniqKey="Famulski K" first="K. S." last="Famulski">K. S. Famulski</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Halloran, P F" sort="Halloran, P F" uniqKey="Halloran P" first="P. F." last="Halloran">P. F. Halloran</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">13-0206839</idno>
<date when="2013">2013</date>
<idno type="stanalyst">PASCAL 13-0206839 INIST</idno>
<idno type="RBID">Pascal:13-0206839</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000A14</idno>
<idno type="wicri:Area/PascalFrancis/Curation">005461</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants</title>
<author><name sortKey="Sellares, J" sort="Sellares, J" uniqKey="Sellares J" first="J." last="Sellares">J. Sellares</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Servei de Nefrologia, Hospital de la Vall d'Hebron</s1>
<s2>Barcelona</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
</affiliation>
</author>
<author><name sortKey="Reeve, J" sort="Reeve, J" uniqKey="Reeve J" first="J." last="Reeve">J. Reeve</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Loupy, A" sort="Loupy, A" uniqKey="Loupy A" first="A." last="Loupy">A. Loupy</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Kidney Transplant Department, Necker Hospital</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Mengel, M" sort="Mengel, M" uniqKey="Mengel M" first="M." last="Mengel">M. Mengel</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Sis, B" sort="Sis, B" uniqKey="Sis B" first="B." last="Sis">B. Sis</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Skene, A" sort="Skene, A" uniqKey="Skene A" first="A." last="Skene">A. Skene</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Anatomical Pathology, Austin Hospital</s1>
<s2>Heidelberg, Victoria</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
</affiliation>
</author>
<author><name sortKey="De Freitas, D G" sort="De Freitas, D G" uniqKey="De Freitas D" first="D. G." last="De Freitas">D. G. De Freitas</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Renal Medicine, Manchester Royal Infirmary</s1>
<s2>Manchester</s2>
<s3>GBR</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
</affiliation>
</author>
<author><name sortKey="Kreepala, C" sort="Kreepala, C" uniqKey="Kreepala C" first="C." last="Kreepala">C. Kreepala</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Hidalgo, L G" sort="Hidalgo, L G" uniqKey="Hidalgo L" first="L. G." last="Hidalgo">L. G. Hidalgo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Famulski, K S" sort="Famulski, K S" uniqKey="Famulski K" first="K. S." last="Famulski">K. S. Famulski</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Halloran, P F" sort="Halloran, P F" uniqKey="Halloran P" first="P. F." last="Halloran">P. F. Halloran</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">American journal of transplantation : (Print)</title>
<title level="j" type="abbreviated">Am. j. transplant. : (Print)</title>
<idno type="ISSN">1600-6135</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">American journal of transplantation : (Print)</title>
<title level="j" type="abbreviated">Am. j. transplant. : (Print)</title>
<idno type="ISSN">1600-6135</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term>
<term>Graft(material)</term>
<term>Homotransplantation</term>
<term>Human</term>
<term>Kidney</term>
<term>Molecular biology</term>
<term>Molecular diagnostic</term>
<term>Rejection</term>
<term>Treatment</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Biologie moléculaire</term>
<term>Anticorps</term>
<term>Rejet</term>
<term>Homotransplantation</term>
<term>Greffon</term>
<term>Homme</term>
<term>Rein</term>
<term>Traitement</term>
<term>Diagnostic moléculaire</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Antibody-mediated rejection is the major cause of kidney transplant failure, but the histology-based diagnostic system misses most cases due to its requirement for C4d positivity. We hypothesized that gene expression data could be used to test biopsies for the presence of antibody-mediated rejection. To develop a molecular test, we prospectively assigned diagnoses, including C4d-negative antibody-mediated rejection, to 403 indication biopsies from 315 patients, based on histology (microcirculation lesions) and donor-specific HLA antibody. We then used microarray data to develop classifiers that assigned antibody-mediated rejection scores to each biopsy. The transcripts distinguishing antibody-mediated rejection from other conditions were mostly expressed in endothelial cells or NK cells, or were IFNG-inducible. The scores correlated with the presence of microcirculation lesions and donor-specific antibody. Of 45 biopsies with scores >0.5, 39 had been diagnosed as antibody-mediated rejection on the basis of histology and donor-specific antibody. High scores were also associated with unanimity among pathologists that antibody-mediated rejection was present. The molecular score also strongly predicted future graft loss in Cox regression analysis. We conclude that microarray assessment of gene expression can assign a probability of ABMR to transplant biopsies without knowledge of HLA antibody status, histology, or C4d staining, and predicts future failure.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1600-6135</s0>
</fA01>
<fA03 i2="1"><s0>Am. j. transplant. : (Print)</s0>
</fA03>
<fA05><s2>13</s2>
</fA05>
<fA06><s2>4</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>SELLARES (J.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>REEVE (J.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>LOUPY (A.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>MENGEL (M.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>SIS (B.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>SKENE (A.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>DE FREITAS (D. G.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>KREEPALA (C.)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>HIDALGO (L. G.)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>FAMULSKI (K. S.)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>HALLORAN (P. F.)</s1>
</fA11>
<fA14 i1="01"><s1>Alberta Transplant Applied Genomics Centre</s1>
<s2>Edmonton, Alberta</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Servei de Nefrologia, Hospital de la Vall d'Hebron</s1>
<s2>Barcelona</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Laboratory Medicine and Pathology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Kidney Transplant Department, Necker Hospital</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Department of Anatomical Pathology, Austin Hospital</s1>
<s2>Heidelberg, Victoria</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Department of Renal Medicine, Manchester Royal Infirmary</s1>
<s2>Manchester</s2>
<s3>GBR</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="07"><s1>Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta</s1>
<s2>Edmonton, AB</s2>
<s3>CAN</s3>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
</fA14>
<fA20><s1>971-983</s1>
</fA20>
<fA21><s1>2013</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>27587</s2>
<s5>354000504184910190</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2013 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>63 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>13-0206839</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>American journal of transplantation : (Print)</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Antibody-mediated rejection is the major cause of kidney transplant failure, but the histology-based diagnostic system misses most cases due to its requirement for C4d positivity. We hypothesized that gene expression data could be used to test biopsies for the presence of antibody-mediated rejection. To develop a molecular test, we prospectively assigned diagnoses, including C4d-negative antibody-mediated rejection, to 403 indication biopsies from 315 patients, based on histology (microcirculation lesions) and donor-specific HLA antibody. We then used microarray data to develop classifiers that assigned antibody-mediated rejection scores to each biopsy. The transcripts distinguishing antibody-mediated rejection from other conditions were mostly expressed in endothelial cells or NK cells, or were IFNG-inducible. The scores correlated with the presence of microcirculation lesions and donor-specific antibody. Of 45 biopsies with scores >0.5, 39 had been diagnosed as antibody-mediated rejection on the basis of histology and donor-specific antibody. High scores were also associated with unanimity among pathologists that antibody-mediated rejection was present. The molecular score also strongly predicted future graft loss in Cox regression analysis. We conclude that microarray assessment of gene expression can assign a probability of ABMR to transplant biopsies without knowledge of HLA antibody status, histology, or C4d staining, and predicts future failure.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B25</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Biologie moléculaire</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Molecular biology</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Biología molecular</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Anticorps</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Antibody</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Anticuerpo</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Rejet</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Rejection</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Rechazo</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Homotransplantation</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Homotransplantation</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Homotrasplante</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Greffon</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Graft(material)</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Injerto(material)</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Homme</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Human</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Hombre</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Rein</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Kidney</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Riñón</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Traitement</s0>
<s5>25</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Treatment</s0>
<s5>25</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Tratamiento</s0>
<s5>25</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Diagnostic moléculaire</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Molecular diagnostic</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Chirurgie</s0>
<s5>37</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Surgery</s0>
<s5>37</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Cirugía</s0>
<s5>37</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Greffe</s0>
<s5>38</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Graft</s0>
<s5>38</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Injerto</s0>
<s5>38</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Transplantation</s0>
<s5>39</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Transplantation</s0>
<s5>39</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Trasplantación</s0>
<s5>39</s5>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Appareil urinaire</s0>
<s5>40</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Urinary system</s0>
<s5>40</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato urinario</s0>
<s5>40</s5>
</fC07>
<fN21><s1>189</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/PascalFrancis/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005461 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 005461 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    PascalFrancis
   |étape=   Curation
   |type=    RBID
   |clé=     Pascal:13-0206839
   |texte=   Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les relations entre la France et l'Australie

Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants

Molecular Diagnosis of Antibody-Mediated Rejection in Human Kidney Transplants

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri