Growth of human mesenchymal stem cells (MSCs) on films of enzymatically modified chitosan.
Identifieur interne : 000011 ( PubMed/Checkpoint ); précédent : 000010; suivant : 000012Growth of human mesenchymal stem cells (MSCs) on films of enzymatically modified chitosan.
Auteurs : Abdulhadi Aljawish [France] ; Lionel Muniglia [France] ; Isabelle Chevalot [France]Source :
- Biotechnology progress [ 1520-6033 ] ; 2016.
Abstract
Mesenchymal stem cells (MSCs) are known to be an attractive cell source for tissue engineering and regenerative medicine. One of the main limiting steps for clinical use or biotechnological purposes is the expansion step. The research of compatible biomaterials for MSCs expansion is recently regarded as an attractive topic. The aim of this study was to create new functional biomaterial for MSCs expansion by evaluating the impact of chitosan derivative films modified by enzymatic approach. First, chitosan particles were enzymatically modified with ferulic acid (FA) or ethyl ferulate (EF) under an eco-friendly procedure. Then, films of chitosan and its modified derivatives were prepared and evaluated by physicochemical and biological properties. Results showed that the enzymatic grafting of FA or EF onto chitosan significantly increased hydrophobic and antioxidant properties of chitosan films. The MSCs cell viability on chitosan derivative films also increased depending on the film thickness and the quantity of grafted phenols. Furthermore, the cytotoxicity test showed the absence of toxic effect of chitosan derivative films towards MSCs cells. Cell morphology showed a well attached and spread phenotype of MSCs cells on chitosan derivative films. On the other hand, due to the higher phenol content of FA-chitosan films, their hydrophobic, antioxidant properties and cell adhesion were improved in comparison with those of EF-chitosan films. Finally, this enzymatic process can be considered as a promising process to favor MSCs cell growth as well as to create useful biomaterials for biomedical applications especially for tissue engineering. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:491-500, 2016.
DOI: 10.1002/btpr.2216
PubMed: 26701830
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:26701830Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Growth of human mesenchymal stem cells (MSCs) on films of enzymatically modified chitosan.</title><author><name sortKey="Aljawish, Abdulhadi" sort="Aljawish, Abdulhadi" uniqKey="Aljawish A" first="Abdulhadi" last="Aljawish">Abdulhadi Aljawish</name><affiliation wicri:level="4"><nlm:affiliation>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Muniglia, Lionel" sort="Muniglia, Lionel" uniqKey="Muniglia L" first="Lionel" last="Muniglia">Lionel Muniglia</name><affiliation wicri:level="4"><nlm:affiliation>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Chevalot, Isabelle" sort="Chevalot, Isabelle" uniqKey="Chevalot I" first="Isabelle" last="Chevalot">Isabelle Chevalot</name><affiliation wicri:level="4"><nlm:affiliation>Université De Lorraine, Laboratoire Réactions Et Génie Des Procédés (LRGP-CNRS-UMR 7274), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université De Lorraine, Laboratoire Réactions Et Génie Des Procédés (LRGP-CNRS-UMR 7274), TSA40602-F-54518, Vandœuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26701830</idno><idno type="pmid">26701830</idno><idno type="doi">10.1002/btpr.2216</idno><idno type="wicri:Area/PubMed/Corpus">000013</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000013</idno><idno type="wicri:Area/PubMed/Curation">000013</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000013</idno><idno type="wicri:Area/PubMed/Checkpoint">000013</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000013</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Growth of human mesenchymal stem cells (MSCs) on films of enzymatically modified chitosan.</title><author><name sortKey="Aljawish, Abdulhadi" sort="Aljawish, Abdulhadi" uniqKey="Aljawish A" first="Abdulhadi" last="Aljawish">Abdulhadi Aljawish</name><affiliation wicri:level="4"><nlm:affiliation>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Muniglia, Lionel" sort="Muniglia, Lionel" uniqKey="Muniglia L" first="Lionel" last="Muniglia">Lionel Muniglia</name><affiliation wicri:level="4"><nlm:affiliation>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Chevalot, Isabelle" sort="Chevalot, Isabelle" uniqKey="Chevalot I" first="Isabelle" last="Chevalot">Isabelle Chevalot</name><affiliation wicri:level="4"><nlm:affiliation>Université De Lorraine, Laboratoire Réactions Et Génie Des Procédés (LRGP-CNRS-UMR 7274), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université De Lorraine, Laboratoire Réactions Et Génie Des Procédés (LRGP-CNRS-UMR 7274), TSA40602-F-54518, Vandœuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author></analytic><series><title level="j">Biotechnology progress</title><idno type="eISSN">1520-6033</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mesenchymal stem cells (MSCs) are known to be an attractive cell source for tissue engineering and regenerative medicine. One of the main limiting steps for clinical use or biotechnological purposes is the expansion step. The research of compatible biomaterials for MSCs expansion is recently regarded as an attractive topic. The aim of this study was to create new functional biomaterial for MSCs expansion by evaluating the impact of chitosan derivative films modified by enzymatic approach. First, chitosan particles were enzymatically modified with ferulic acid (FA) or ethyl ferulate (EF) under an eco-friendly procedure. Then, films of chitosan and its modified derivatives were prepared and evaluated by physicochemical and biological properties. Results showed that the enzymatic grafting of FA or EF onto chitosan significantly increased hydrophobic and antioxidant properties of chitosan films. The MSCs cell viability on chitosan derivative films also increased depending on the film thickness and the quantity of grafted phenols. Furthermore, the cytotoxicity test showed the absence of toxic effect of chitosan derivative films towards MSCs cells. Cell morphology showed a well attached and spread phenotype of MSCs cells on chitosan derivative films. On the other hand, due to the higher phenol content of FA-chitosan films, their hydrophobic, antioxidant properties and cell adhesion were improved in comparison with those of EF-chitosan films. Finally, this enzymatic process can be considered as a promising process to favor MSCs cell growth as well as to create useful biomaterials for biomedical applications especially for tissue engineering. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:491-500, 2016.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Data-Review"><PMID Version="1">26701830</PMID><DateCreated><Year>2016</Year><Month>04</Month><Day>23</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6033</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol. Prog.</ISOAbbreviation></Journal><ArticleTitle>Growth of human mesenchymal stem cells (MSCs) on films of enzymatically modified chitosan.</ArticleTitle><Pagination><MedlinePgn>491-500</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/btpr.2216</ELocationID><Abstract><AbstractText>Mesenchymal stem cells (MSCs) are known to be an attractive cell source for tissue engineering and regenerative medicine. One of the main limiting steps for clinical use or biotechnological purposes is the expansion step. The research of compatible biomaterials for MSCs expansion is recently regarded as an attractive topic. The aim of this study was to create new functional biomaterial for MSCs expansion by evaluating the impact of chitosan derivative films modified by enzymatic approach. First, chitosan particles were enzymatically modified with ferulic acid (FA) or ethyl ferulate (EF) under an eco-friendly procedure. Then, films of chitosan and its modified derivatives were prepared and evaluated by physicochemical and biological properties. Results showed that the enzymatic grafting of FA or EF onto chitosan significantly increased hydrophobic and antioxidant properties of chitosan films. The MSCs cell viability on chitosan derivative films also increased depending on the film thickness and the quantity of grafted phenols. Furthermore, the cytotoxicity test showed the absence of toxic effect of chitosan derivative films towards MSCs cells. Cell morphology showed a well attached and spread phenotype of MSCs cells on chitosan derivative films. On the other hand, due to the higher phenol content of FA-chitosan films, their hydrophobic, antioxidant properties and cell adhesion were improved in comparison with those of EF-chitosan films. Finally, this enzymatic process can be considered as a promising process to favor MSCs cell growth as well as to create useful biomaterials for biomedical applications especially for tissue engineering. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:491-500, 2016.</AbstractText><CopyrightInformation>© 2016 American Institute of Chemical Engineers.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aljawish</LastName><ForeName>Abdulhadi</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muniglia</LastName><ForeName>Lionel</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Université De Lorraine, Laboratoire D'ingénierie Des Biomolécules (LIBio), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chevalot</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Université De Lorraine, Laboratoire Réactions Et Génie Des Procédés (LRGP-CNRS-UMR 7274), TSA40602-F-54518, Vandœuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ABTS</Keyword><Keyword MajorTopicYN="N">cell adhesion</Keyword><Keyword MajorTopicYN="N">chitosan films</Keyword><Keyword MajorTopicYN="N">enzymatic modification</Keyword><Keyword MajorTopicYN="N">mesenchymal stem cells</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>8</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>11</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2016</Year><Month>2</Month><Day>4</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>12</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>12</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>12</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26701830</ArticleId><ArticleId IdType="doi">10.1002/btpr.2216</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Vandœuvre-lès-Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="France"><region name="Grand Est"><name sortKey="Aljawish, Abdulhadi" sort="Aljawish, Abdulhadi" uniqKey="Aljawish A" first="Abdulhadi" last="Aljawish">Abdulhadi Aljawish</name></region><name sortKey="Chevalot, Isabelle" sort="Chevalot, Isabelle" uniqKey="Chevalot I" first="Isabelle" last="Chevalot">Isabelle Chevalot</name><name sortKey="Muniglia, Lionel" sort="Muniglia, Lionel" uniqKey="Muniglia L" first="Lionel" last="Muniglia">Lionel Muniglia</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/LrgpV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000011 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000011 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Lorraine
|area= LrgpV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:26701830
|texte= Growth of human mesenchymal stem cells (MSCs) on films of enzymatically modified chitosan.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:26701830" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a LrgpV1
| This area was generated with Dilib version V0.6.32. |  |