Coumarin-like fluorescent molecular rotors for bioactive polymers probing.
Identifieur interne : 000025 ( Ncbi/Merge ); précédent : 000024; suivant : 000026Coumarin-like fluorescent molecular rotors for bioactive polymers probing.
Auteurs : P. Even [France] ; F. Chaubet ; D. Letourneur ; M L Viriot ; M C CarréSource :
- Biorheology [ 0006-355X ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Endothélium.
- pharmacologie : Polyosides.
- Colorants fluorescents, Coumarines, Division cellulaire, Endothélium, Humains, Lignée cellulaire, Microscopie de fluorescence, Polymères, Polyosides, Sondes moléculaires.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Coumarins, Polymers, Polysaccharides.
- cytology : Endothelium.
- drug effects : Cell Division, Endothelium.
- chemical , pharmacology : Polysaccharides.
- Cell Line, Fluorescent Dyes, Humans, Microscopy, Fluorescence, Molecular Probes.
Abstract
Polysaccharides are interesting and often essential macromolecules but are difficult to analyse due to their lack of convenient chromophores. We propose an efficient labelling procedure for polysaccharides such as functionalized dextrans with coumarin derivatives: the fluorescent tracers present inter alia properties of emission of fluorescence dependent on the molecular environment (polarity, viscosity, temperature, pH, etc.). Hence, with in mind the understanding of cell-polysaccharide interactions, the labelled polymers were studied by in vitro tests on a line of endothelial cells sensitive to the proliferative effect of these dextran polysaccharides. Using 3D fluorescence microscopy, the fixation and internalization of fluorescent functionalized dextrans were observed in endothelial cells.
PubMed: 12454414
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pubmed:12454414Le document en format XML
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<term>Endothelium (cytology)</term>
<term>Endothelium (drug effects)</term>
<term>Fluorescent Dyes</term>
<term>Humans</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Probes</term>
<term>Polymers (chemistry)</term>
<term>Polysaccharides (chemistry)</term>
<term>Polysaccharides (pharmacology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Colorants fluorescents</term>
<term>Coumarines ()</term>
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<term>Endothélium (cytologie)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Microscopie de fluorescence</term>
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<term>Polyosides ()</term>
<term>Polyosides (pharmacologie)</term>
<term>Sondes moléculaires</term>
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<term>Polymers</term>
<term>Polysaccharides</term>
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<term>Lignée cellulaire</term>
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<front><div type="abstract" xml:lang="en">Polysaccharides are interesting and often essential macromolecules but are difficult to analyse due to their lack of convenient chromophores. We propose an efficient labelling procedure for polysaccharides such as functionalized dextrans with coumarin derivatives: the fluorescent tracers present inter alia properties of emission of fluorescence dependent on the molecular environment (polarity, viscosity, temperature, pH, etc.). Hence, with in mind the understanding of cell-polysaccharide interactions, the labelled polymers were studied by in vitro tests on a line of endothelial cells sensitive to the proliferative effect of these dextran polysaccharides. Using 3D fluorescence microscopy, the fixation and internalization of fluorescent functionalized dextrans were observed in endothelial cells.</div>
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<Abstract><AbstractText>Polysaccharides are interesting and often essential macromolecules but are difficult to analyse due to their lack of convenient chromophores. We propose an efficient labelling procedure for polysaccharides such as functionalized dextrans with coumarin derivatives: the fluorescent tracers present inter alia properties of emission of fluorescence dependent on the molecular environment (polarity, viscosity, temperature, pH, etc.). Hence, with in mind the understanding of cell-polysaccharide interactions, the labelled polymers were studied by in vitro tests on a line of endothelial cells sensitive to the proliferative effect of these dextran polysaccharides. Using 3D fluorescence microscopy, the fixation and internalization of fluorescent functionalized dextrans were observed in endothelial cells.</AbstractText>
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