Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging.
Identifieur interne : 000346 ( Ncbi/Checkpoint ); précédent : 000345; suivant : 000347Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging.
Auteurs : Martyna Michalska [Pologne] ; Anna Florczak [Pologne] ; Hanna Dams-Kozlowska [Pologne] ; Jacek Gapinski [Pologne] ; Stefan Jurga [Pologne] ; Raphaël Schneider [France]Source :
- Acta biomaterialia [ 1878-7568 ] ; 2016.
Abstract
In this paper, the synthesis of alloyed CuInZnxS2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS2 core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells.
DOI: 10.1016/j.actbio.2016.02.002
PubMed: 26850146
Affiliations:
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NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Laboratoire Réactions et Génie de Procédés (LRGP), Université de Lorraine, CNRS, UMR 7274, 1 rue Grandville, BP 20451, 54001 Nancy Cedex, France; NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan</wicri:regionArea><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Florczak, Anna" sort="Florczak, Anna" uniqKey="Florczak A" first="Anna" last="Florczak">Anna Florczak</name><affiliation wicri:level="1"><nlm:affiliation>NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan 61-866, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan 61-866</wicri:regionArea><wicri:noRegion>Poznan 61-866</wicri:noRegion></affiliation></author><author><name sortKey="Dams Kozlowska, Hanna" sort="Dams Kozlowska, Hanna" uniqKey="Dams Kozlowska H" first="Hanna" last="Dams-Kozlowska">Hanna Dams-Kozlowska</name><affiliation wicri:level="1"><nlm:affiliation>Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan 61-866, Poland; Department of Diagnostics and Cancer Immunology, Greater Poland Cancer Centre, Poznan 61-866, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan 61-866, Poland; Department of Diagnostics and Cancer Immunology, Greater Poland Cancer Centre, Poznan 61-866</wicri:regionArea><wicri:noRegion>Poznan 61-866</wicri:noRegion></affiliation></author><author><name sortKey="Gapinski, Jacek" sort="Gapinski, Jacek" uniqKey="Gapinski J" first="Jacek" last="Gapinski">Jacek Gapinski</name><affiliation wicri:level="1"><nlm:affiliation>NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Molecular Biophysics Division, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Molecular Biophysics Division, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan</wicri:regionArea><wicri:noRegion>61-614 Poznan</wicri:noRegion></affiliation></author><author><name sortKey="Jurga, Stefan" sort="Jurga, Stefan" uniqKey="Jurga S" first="Stefan" last="Jurga">Stefan Jurga</name><affiliation wicri:level="1"><nlm:affiliation>NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan</wicri:regionArea><wicri:noRegion>61-614 Poznan</wicri:noRegion></affiliation></author><author><name sortKey="Schneider, Raphael" sort="Schneider, Raphael" uniqKey="Schneider R" first="Raphaël" last="Schneider">Raphaël Schneider</name><affiliation wicri:level="4"><nlm:affiliation>Laboratoire Réactions et Génie de Procédés (LRGP), Université de Lorraine, CNRS, UMR 7274, 1 rue Grandville, BP 20451, 54001 Nancy Cedex, France. Electronic address: raphael.schneider@univ-lorraine.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Réactions et Génie de Procédés (LRGP), Université de Lorraine, CNRS, UMR 7274, 1 rue Grandville, BP 20451, 54001 Nancy Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author></analytic><series><title level="j">Acta biomaterialia</title><idno type="eISSN">1878-7568</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this paper, the synthesis of alloyed CuInZnxS2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS2 core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells.</div></front></TEI><affiliations><list><country><li>France</li><li>Pologne</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Metz</li><li>Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="Pologne"><region name="Grand Est"><name sortKey="Michalska, Martyna" sort="Michalska, Martyna" uniqKey="Michalska M" first="Martyna" last="Michalska">Martyna Michalska</name></region><name sortKey="Dams Kozlowska, Hanna" sort="Dams Kozlowska, Hanna" uniqKey="Dams Kozlowska H" first="Hanna" last="Dams-Kozlowska">Hanna Dams-Kozlowska</name><name sortKey="Florczak, Anna" sort="Florczak, Anna" uniqKey="Florczak A" first="Anna" last="Florczak">Anna Florczak</name><name sortKey="Gapinski, Jacek" sort="Gapinski, Jacek" uniqKey="Gapinski J" first="Jacek" last="Gapinski">Jacek Gapinski</name><name sortKey="Jurga, Stefan" sort="Jurga, Stefan" uniqKey="Jurga S" first="Stefan" last="Jurga">Stefan Jurga</name></country><country name="France"><region name="Grand Est"><name sortKey="Schneider, Raphael" sort="Schneider, Raphael" uniqKey="Schneider R" first="Raphaël" last="Schneider">Raphaël Schneider</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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