Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases
Identifieur interne : 000450 ( Main/Exploration ); précédent : 000449; suivant : 000451Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases
Auteurs : Karsten König [Allemagne] ; Marco Speicher [Allemagne] ; Martin J. Köhler [Allemagne] ; Rüdiger Scharenberg [Allemagne] ; Martin Kaatz [Allemagne]Source :
- Journal of Biophotonics [ 1864-063X ] ; 2010-12.
English descriptors
- KwdEn :
- Teeft :
- Acantholytic cells, Actinic keratosis, Biophotonics, Blood vessels, Bullous, Cell clusters, Clinical application, Connective tissue diseases, Cortex hypoechoic width, Cortex system, Coverslip, Depth extension, Dermainspect, Dermal, Dermatol, Dermatology, Dermatosis, Dermis, Dermoscopic, Dermoscopic image, Dermoscopic images, Dermoscopy, Entry echo, Epidermis, Full article, Gmbh, Hemangioma, High frequencies, High frequency, Histological image, Human skin, Hypoechoic, Hypoechoic depth extension, Hypoechoic structure, Imaging, Inflammatory, Jena, Jenlab gmbh, Kaatz, Kgaa, Konig, Large cell distances, Laser, Lesion, Male patient, Malignant, Malignant melanoma, Maximum diameter, Melanocytic, Melanocytic nevus, Melanoma, Multiphoton, Multiphoton tomography, Nevus, Online, Online color, Optical biopsies, Pemphigus vulgaris, Penetration depth, Psoriatic lesion, Ring foil, Saarland university, Scan type, Signal depth, Skin cancer, Skin level, Skin surface, Skin tumors, Subcutaneous, Therapy monitoring, Tissue depth, Tomograph, Tomography, Ultrasound, Ultrasound images, Ultrasound imaging, Ultrasound scan, Verlag, Verlag gmbh, Weinheim, Weinheim journal.
Abstract
The first‐ever application of high‐frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state‐of‐the‐art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two‐dimensional color images of the skin surface with a magnification up to 70×. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide‐field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 × 0.36 × 0.001 mm3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Url:
DOI: 10.1002/jbio.201000074
Affiliations:
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Biophoton.</title><idno type="ISSN">1864-063X</idno><idno type="eISSN">1864-0648</idno><imprint><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Berlin</pubPlace><date type="published" when="2010-12">2010-12</date><biblScope unit="volume">3</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="759">759</biblScope><biblScope unit="page" to="773">773</biblScope></imprint><idno type="ISSN">1864-063X</idno></series><idno type="istex">50337DBF6837CE079AA340246F296B7360FB7637</idno><idno type="DOI">10.1002/jbio.201000074</idno><idno type="ArticleID">JBIO201000074</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1864-063X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>SHG</term><term>high frequency</term><term>molecular imaging</term><term>multiphoton tomography</term><term>skin</term><term>skin cancer</term><term>two‐photon</term><term>ultrasound</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acantholytic cells</term><term>Actinic keratosis</term><term>Biophotonics</term><term>Blood vessels</term><term>Bullous</term><term>Cell clusters</term><term>Clinical application</term><term>Connective tissue diseases</term><term>Cortex hypoechoic width</term><term>Cortex system</term><term>Coverslip</term><term>Depth extension</term><term>Dermainspect</term><term>Dermal</term><term>Dermatol</term><term>Dermatology</term><term>Dermatosis</term><term>Dermis</term><term>Dermoscopic</term><term>Dermoscopic image</term><term>Dermoscopic images</term><term>Dermoscopy</term><term>Entry echo</term><term>Epidermis</term><term>Full article</term><term>Gmbh</term><term>Hemangioma</term><term>High frequencies</term><term>High frequency</term><term>Histological image</term><term>Human skin</term><term>Hypoechoic</term><term>Hypoechoic depth extension</term><term>Hypoechoic structure</term><term>Imaging</term><term>Inflammatory</term><term>Jena</term><term>Jenlab gmbh</term><term>Kaatz</term><term>Kgaa</term><term>Konig</term><term>Large cell distances</term><term>Laser</term><term>Lesion</term><term>Male patient</term><term>Malignant</term><term>Malignant melanoma</term><term>Maximum diameter</term><term>Melanocytic</term><term>Melanocytic nevus</term><term>Melanoma</term><term>Multiphoton</term><term>Multiphoton tomography</term><term>Nevus</term><term>Online</term><term>Online color</term><term>Optical biopsies</term><term>Pemphigus vulgaris</term><term>Penetration depth</term><term>Psoriatic lesion</term><term>Ring foil</term><term>Saarland university</term><term>Scan type</term><term>Signal depth</term><term>Skin cancer</term><term>Skin level</term><term>Skin surface</term><term>Skin tumors</term><term>Subcutaneous</term><term>Therapy monitoring</term><term>Tissue depth</term><term>Tomograph</term><term>Tomography</term><term>Ultrasound</term><term>Ultrasound images</term><term>Ultrasound imaging</term><term>Ultrasound scan</term><term>Verlag</term><term>Verlag gmbh</term><term>Weinheim</term><term>Weinheim journal</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The first‐ever application of high‐frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state‐of‐the‐art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two‐dimensional color images of the skin surface with a magnification up to 70×. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide‐field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 × 0.36 × 0.001 mm3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Sarre (Land)</li></region><settlement><li>Sarrebruck</li></settlement></list><tree><country name="Allemagne"><region name="Sarre (Land)"><name sortKey="Konig, Karsten" sort="Konig, Karsten" uniqKey="Konig K" first="Karsten" last="König">Karsten König</name></region><name sortKey="Kaatz, Martin" sort="Kaatz, Martin" uniqKey="Kaatz M" first="Martin" last="Kaatz">Martin Kaatz</name><name sortKey="Kohler, Martin J" sort="Kohler, Martin J" uniqKey="Kohler M" first="Martin J." last="Köhler">Martin J. Köhler</name><name sortKey="Konig, Karsten" sort="Konig, Karsten" uniqKey="Konig K" first="Karsten" last="König">Karsten König</name><name sortKey="Scharenberg, Rudiger" sort="Scharenberg, Rudiger" uniqKey="Scharenberg R" first="Rüdiger" last="Scharenberg">Rüdiger Scharenberg</name><name sortKey="Speicher, Marco" sort="Speicher, Marco" uniqKey="Speicher M" first="Marco" last="Speicher">Marco Speicher</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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