Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases
Identifieur interne : 000848 ( Istex/Corpus ); précédent : 000847; suivant : 000849Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases
Auteurs : Karsten König ; Marco Speicher ; Martin J. Köhler ; Rüdiger Scharenberg ; Martin KaatzSource :
- Journal of Biophotonics [ 1864-063X ] ; 2010-12.
English descriptors
- KwdEn :
- Teeft :
- Acantholytic cells, Actinic keratosis, Biophotonics, Blood vessels, Bullous, Cell clusters, Clinical application, Connective tissue diseases, Cortex hypoechoic width, Cortex system, Coverslip, Depth extension, Dermainspect, Dermal, Dermatol, Dermatology, Dermatosis, Dermis, Dermoscopic, Dermoscopic image, Dermoscopic images, Dermoscopy, Entry echo, Epidermis, Full article, Gmbh, Hemangioma, High frequencies, High frequency, Histological image, Human skin, Hypoechoic, Hypoechoic depth extension, Hypoechoic structure, Imaging, Inflammatory, Jena, Jenlab gmbh, Kaatz, Kgaa, Konig, Large cell distances, Laser, Lesion, Male patient, Malignant, Malignant melanoma, Maximum diameter, Melanocytic, Melanocytic nevus, Melanoma, Multiphoton, Multiphoton tomography, Nevus, Online, Online color, Optical biopsies, Pemphigus vulgaris, Penetration depth, Psoriatic lesion, Ring foil, Saarland university, Scan type, Signal depth, Skin cancer, Skin level, Skin surface, Skin tumors, Subcutaneous, Therapy monitoring, Tissue depth, Tomograph, Tomography, Ultrasound, Ultrasound images, Ultrasound imaging, Ultrasound scan, Verlag, Verlag gmbh, Weinheim, Weinheim journal.
Abstract
The first‐ever application of high‐frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state‐of‐the‐art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two‐dimensional color images of the skin surface with a magnification up to 70×. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide‐field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 × 0.36 × 0.001 mm3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Url:
DOI: 10.1002/jbio.201000074
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ISTEX:50337DBF6837CE079AA340246F296B7360FB7637Le document en format XML
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Dermoscopy provides two‐dimensional color images of the skin surface with a magnification up to 70×. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide‐field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 × 0.36 × 0.001 mm3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments. (© 2010 WILEY‐VCH Verlag GmbH & Co. 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Dermoscopy provides two‐dimensional color images of the skin surface with a magnification up to 70×. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide‐field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 × 0.36 × 0.001 mm3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>high frequency</term></item><item><term>ultrasound</term></item><item><term>multiphoton tomography</term></item><item><term>skin</term></item><item><term>SHG</term></item><item><term>molecular imaging</term></item><item><term>two‐photon</term></item><item><term>skin cancer</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Editor's Choice</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-06-02">Received</change><change when="2010-07-13">Registration</change><change when="2010-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/50337DBF6837CE079AA340246F296B7360FB7637/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>WILEY‐VCH Verlag</publisherName><publisherLoc>Berlin</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1864-0648</doi><issn type="print">1864-063X</issn><issn type="electronic">1864-0648</issn><idGroup><id type="product" value="JBIO"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF BIOPHOTONICS">Journal of Biophotonics</title><title type="short">J. Biophoton.</title></titleGroup></publicationMeta><publicationMeta level="part" position="120"><doi origin="wiley" registered="yes">10.1002/jbio.v3.12</doi><titleGroup><title type="specialIssueTitle">Optical Bioimaging and Neuroimaging</title></titleGroup><numberingGroup><numbering type="journalVolume" number="3">3</numbering><numbering type="journalIssue">12</numbering></numberingGroup><creators><creator xml:id="sped1" creatorRole="sponsoringEditor"><personName><givenNames>Mikhail</givenNames><familyName>Kirillin</familyName></personName></creator><creator xml:id="sped2" creatorRole="sponsoringEditor"><personName><givenNames>Karsten</givenNames><familyName>König</familyName></personName></creator><creator xml:id="sped3" creatorRole="sponsoringEditor"><personName><givenNames>Natalia</givenNames><familyName>Shakhova</familyName></personName></creator><creator xml:id="sped4" creatorRole="sponsoringEditor"><personName><givenNames>Bruce</givenNames><familyName>Tromberg</familyName></personName></creator><creator xml:id="sped5" creatorRole="sponsoringEditor"><personName><givenNames>Alexey</givenNames><familyName>Semyanov</familyName></personName></creator></creators><coverDate startDate="2010-12">December 2010</coverDate></publicationMeta><publicationMeta level="unit" type="editorial" position="6" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jbio.201000074</doi><idGroup><id type="unit" value="JBIO201000074"></id></idGroup><countGroup><count type="pageTotal" number="15"></count></countGroup><titleGroup><title type="articleCategory">Editor's Choice</title><title type="tocHeading1">Editor's Choice</title></titleGroup><copyright ownership="publisher">Copyright © 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</copyright><eventGroup><event type="manuscriptReceived" date="2010-06-02"></event><event type="manuscriptRevised" date="2010-07-12"></event><event type="manuscriptAccepted" date="2010-07-13"></event><event type="firstOnline" date="2010-11-16"></event><event type="publishedOnlineAcceptedOrEarlyUnpaginated" date="2010-08-02"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.26 mode:FullText" date="2010-11-16"></event><event type="publishedOnlineFinalForm" date="2010-11-16"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-24"></event></eventGroup><numberingGroup><numbering type="pageFirst">759</numbering><numbering type="pageLast">773</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:JBIO.JBIO201000074.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="12"></count><count type="tableTotal" number="8"></count><count type="referenceTotal" number="28"></count></countGroup><titleGroup><title type="main" xml:lang="en">Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases</title><title type="short" xml:lang="en">Clinical application of MPT in combination with high‐frequency ultrasound</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#a1 #a2"><personName><givenNames>Karsten</givenNames><familyName>König</familyName></personName></creator><creator xml:id="au2" creatorRole="author"><personName><givenNames>Marco</givenNames><familyName>Speicher</familyName></personName><contactDetails><email>info@jenlab.de</email></contactDetails></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#a4"><personName><givenNames>Martin J.</givenNames><familyName>Köhler</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#a3"><personName><givenNames>Rüdiger</givenNames><familyName>Scharenberg</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#a4"><personName><givenNames>Martin</givenNames><familyName>Kaatz</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="DE" type="organization"><unparsedAffiliation>JenLab GmbH, Schillerstraße 1, 07745 Jena and Campus A1.2, 66123 Saarbrücken, Germany</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="DE" type="organization"><unparsedAffiliation>Department of Biophotonics and Laser Technology, Faculty of Physics and Mechatronics, Saarland University, Campus A5.1, 66123 Saarbrücken, Germany</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="DE" type="organization"><unparsedAffiliation>Taberna pro medicum GmbH, Lüneburg, Germany</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="DE" type="organization"><unparsedAffiliation>Department of Dermatology and Allergology, University Hospital Jena, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">high frequency</keyword><keyword xml:id="kwd2">ultrasound</keyword><keyword xml:id="kwd3">multiphoton tomography</keyword><keyword xml:id="kwd4">skin</keyword><keyword xml:id="kwd5">SHG</keyword><keyword xml:id="kwd6">molecular imaging</keyword><keyword xml:id="kwd7">two‐photon</keyword><keyword xml:id="kwd8">skin cancer</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The first‐ever application of high‐frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state‐of‐the‐art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two‐dimensional color images of the skin surface with a magnification up to 70×. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide‐field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 × 0.36 × 0.001 mm<sup>3</sup> horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Clinical application of MPT in combination with high‐frequency ultrasound</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases</title></titleInfo><name type="personal"><namePart type="given">Karsten</namePart><namePart type="family">König</namePart><affiliation>JenLab GmbH, Schillerstraße 1, 07745 Jena and Campus A1.2, 66123 Saarbrücken, Germany</affiliation><affiliation>Department of Biophotonics and Laser Technology, Faculty of Physics and Mechatronics, Saarland University, Campus A5.1, 66123 Saarbrücken, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marco</namePart><namePart type="family">Speicher</namePart><affiliation>E-mail: info@jenlab.de</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Martin J.</namePart><namePart type="family">Köhler</namePart><affiliation>Department of Dermatology and Allergology, University Hospital Jena, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rüdiger</namePart><namePart type="family">Scharenberg</namePart><affiliation>Taberna pro medicum GmbH, Lüneburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Martin</namePart><namePart type="family">Kaatz</namePart><affiliation>Department of Dermatology and Allergology, University Hospital Jena, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="editorial" displayLabel="editorial"></genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Berlin</placeTerm></place><dateIssued encoding="w3cdtf">2010-12</dateIssued><dateCaptured encoding="w3cdtf">2010-06-02</dateCaptured><dateValid encoding="w3cdtf">2010-07-13</dateValid><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">12</extent><extent unit="tables">8</extent><extent unit="references">28</extent></physicalDescription><abstract lang="en">The first‐ever application of high‐frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state‐of‐the‐art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two‐dimensional color images of the skin surface with a magnification up to 70×. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide‐field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 μm (axial) and 32 μm (lateral). Multiphoton tomography provides 0.36 × 0.36 × 0.001 mm3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 μm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</abstract><subject lang="en"><genre>keywords</genre><topic>high frequency</topic><topic>ultrasound</topic><topic>multiphoton tomography</topic><topic>skin</topic><topic>SHG</topic><topic>molecular imaging</topic><topic>two‐photon</topic><topic>skin cancer</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Biophotonics</title></titleInfo><titleInfo type="abbreviated"><title>J. Biophoton.</title></titleInfo><name type="personal"><namePart type="given">Mikhail</namePart><namePart type="family">Kirillin</namePart></name><name type="personal"><namePart type="given">Karsten</namePart><namePart type="family">König</namePart></name><name type="personal"><namePart type="given">Natalia</namePart><namePart type="family">Shakhova</namePart></name><name type="personal"><namePart type="given">Bruce</namePart><namePart type="family">Tromberg</namePart></name><name type="personal"><namePart type="given">Alexey</namePart><namePart type="family">Semyanov</namePart></name><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Editor's Choice</topic></subject><identifier type="ISSN">1864-063X</identifier><identifier type="eISSN">1864-0648</identifier><identifier type="DOI">10.1002/(ISSN)1864-0648</identifier><identifier type="PublisherID">JBIO</identifier><part><date>2010</date><detail type="title"><title>Optical Bioimaging and Neuroimaging</title></detail><detail type="volume"><caption>vol.</caption><number>3</number></detail><detail type="issue"><caption>no.</caption><number>12</number></detail><extent unit="pages"><start>759</start><end>773</end><total>15</total></extent></part></relatedItem><identifier type="istex">50337DBF6837CE079AA340246F296B7360FB7637</identifier><identifier type="DOI">10.1002/jbio.201000074</identifier><identifier type="ArticleID">JBIO201000074</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2010 WILEY‐VCH Verlag GmbH & Co. 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