Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection.
Identifieur interne : 000952 ( PubMed/Corpus ); précédent : 000951; suivant : 000953Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection.
Auteurs : A. Meyer ; T. Ngiruwonsanga ; G. HenzeSource :
- Analytical and bioanalytical chemistry [ 1618-2650 ] ; 1996.
Abstract
A relatively simple method for quantifying caffeine, theobromine, theophylline and adenine by HPLC with amperometric detection was developed. A C(18)-column and an isocratic elution with phosphate buffer pH 3.5/methanol (90 : 10) were employed for the chromatographic separation of the investigated compounds. The optimal detection potential was +1.4 V. The limits of detection were 0.4 ng for adenine, 1 ng for theophylline and 2.5 ng for caffeine and theobromine. The method was applied to the determination of these purine alkaloids in beverages, tea, coffee and cacao. The determination was carried out directly or after solid-phase extraction.
DOI: 10.1007/s0021663560284
PubMed: 15048370
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Henze</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:15048370</idno><idno type="pmid">15048370</idno><idno type="doi">10.1007/s0021663560284</idno><idno type="wicri:Area/PubMed/Corpus">000952</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000952</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection.</title><author><name sortKey="Meyer, A" sort="Meyer, A" uniqKey="Meyer A" first="A" last="Meyer">A. Meyer</name><affiliation><nlm:affiliation>Fachbereich Chemie der Universität Kaiserslautern und Abteilung für Anorganische und Analytische Chemie der Universität Trier, Postfach 3825, D-54228, Trier, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Ngiruwonsanga, T" sort="Ngiruwonsanga, T" uniqKey="Ngiruwonsanga T" first="T" last="Ngiruwonsanga">T. Ngiruwonsanga</name></author><author><name sortKey="Henze, G" sort="Henze, G" uniqKey="Henze G" first="G" last="Henze">G. Henze</name></author></analytic><series><title level="j">Analytical and bioanalytical chemistry</title><idno type="eISSN">1618-2650</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A relatively simple method for quantifying caffeine, theobromine, theophylline and adenine by HPLC with amperometric detection was developed. A C(18)-column and an isocratic elution with phosphate buffer pH 3.5/methanol (90 : 10) were employed for the chromatographic separation of the investigated compounds. The optimal detection potential was +1.4 V. The limits of detection were 0.4 ng for adenine, 1 ng for theophylline and 2.5 ng for caffeine and theobromine. The method was applied to the determination of these purine alkaloids in beverages, tea, coffee and cacao. The determination was carried out directly or after solid-phase extraction.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">15048370</PMID><DateCreated><Year>2004</Year><Month>03</Month><Day>29</Day></DateCreated><DateRevised><Year>2004</Year><Month>03</Month><Day>29</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1618-2650</ISSN><JournalIssue CitedMedium="Internet"><Volume>356</Volume><Issue>3-4</Issue><PubDate><Year>1996</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Analytical and bioanalytical chemistry</Title><ISOAbbreviation>Anal Bioanal Chem</ISOAbbreviation></Journal><ArticleTitle>Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection.</ArticleTitle><Pagination><MedlinePgn>284-7</MedlinePgn></Pagination><Abstract><AbstractText>A relatively simple method for quantifying caffeine, theobromine, theophylline and adenine by HPLC with amperometric detection was developed. A C(18)-column and an isocratic elution with phosphate buffer pH 3.5/methanol (90 : 10) were employed for the chromatographic separation of the investigated compounds. The optimal detection potential was +1.4 V. The limits of detection were 0.4 ng for adenine, 1 ng for theophylline and 2.5 ng for caffeine and theobromine. The method was applied to the determination of these purine alkaloids in beverages, tea, coffee and cacao. The determination was carried out directly or after solid-phase extraction.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Fachbereich Chemie der Universität Kaiserslautern und Abteilung für Anorganische und Analytische Chemie der Universität Trier, Postfach 3825, D-54228, Trier, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ngiruwonsanga</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Henze</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Anal Bioanal Chem</MedlineTA><NlmUniqueID>101134327</NlmUniqueID><ISSNLinking>1618-2642</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>1995</Year><Month>12</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>1996</Year><Month>03</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>1996</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15048370</ArticleId><ArticleId IdType="doi">10.1007/s0021663560284</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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