Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection.
Identifieur interne : 000952 ( PubMed/Corpus ); précédent : 000951; suivant : 000953Determination of adenine, caffeine, theophylline and theobromine by HPLC with amperometric detection.
Auteurs : A. Meyer ; T. Ngiruwonsanga ; G. HenzeSource :
- Analytical and bioanalytical chemistry [ 1618-2650 ] ; 1996.
Abstract
A relatively simple method for quantifying caffeine, theobromine, theophylline and adenine by HPLC with amperometric detection was developed. A C(18)-column and an isocratic elution with phosphate buffer pH 3.5/methanol (90 : 10) were employed for the chromatographic separation of the investigated compounds. The optimal detection potential was +1.4 V. The limits of detection were 0.4 ng for adenine, 1 ng for theophylline and 2.5 ng for caffeine and theobromine. The method was applied to the determination of these purine alkaloids in beverages, tea, coffee and cacao. The determination was carried out directly or after solid-phase extraction.
DOI: 10.1007/s0021663560284
PubMed: 15048370
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<front><div type="abstract" xml:lang="en">A relatively simple method for quantifying caffeine, theobromine, theophylline and adenine by HPLC with amperometric detection was developed. A C(18)-column and an isocratic elution with phosphate buffer pH 3.5/methanol (90 : 10) were employed for the chromatographic separation of the investigated compounds. The optimal detection potential was +1.4 V. The limits of detection were 0.4 ng for adenine, 1 ng for theophylline and 2.5 ng for caffeine and theobromine. The method was applied to the determination of these purine alkaloids in beverages, tea, coffee and cacao. The determination was carried out directly or after solid-phase extraction.</div>
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<Abstract><AbstractText>A relatively simple method for quantifying caffeine, theobromine, theophylline and adenine by HPLC with amperometric detection was developed. A C(18)-column and an isocratic elution with phosphate buffer pH 3.5/methanol (90 : 10) were employed for the chromatographic separation of the investigated compounds. The optimal detection potential was +1.4 V. The limits of detection were 0.4 ng for adenine, 1 ng for theophylline and 2.5 ng for caffeine and theobromine. The method was applied to the determination of these purine alkaloids in beverages, tea, coffee and cacao. The determination was carried out directly or after solid-phase extraction.</AbstractText>
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