Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cells
Identifieur interne : 000D49 ( PascalFrancis/Corpus ); précédent : 000D48; suivant : 000D50Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cells
Auteurs : W. Q. Lu ; X. N. Chen ; F. Yue ; C. Jenter ; R. Gminski ; X. Y. Li ; H. Xie ; V. Mersch-SundermannSource :
- Mutation research. Genetic toxicology and environmental mutagenesis [ 1383-5718 ] ; 2002.
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Abstract
In the present study, DNA damaging and mutagenic effects of chlorinated drinking water (CDW) extracts obtained from polluted raw water resources were examined in metabolically competent human Hep G2 hepatoma cells using the in vitro micronucleus assay and the single cell gel electrophoresis (SCGE, comet assay). Additionally, the in vivo induction of micronuclei (MN) was studied in polychromatic erythrocytes (PCEs) derived from bone marrow of CDW-treated Wistar rats. Furthermore, we examined the influence of CDW on the lipid peroxidation (LpO) in blood, liver, kidney and testicle of rats. The results demonstrated significant increases of micronucleated PCEs in the bone marrow of rats fed with relatively low CDW doses (33.3 ml/kg body weight per day). Similar effects, i.e. increases of MN frequencies, were found in Hep G2 hepatoma cells after CDW treatment (41 MN/1000 binucleated cells (BNCs) for 167 ml CDW) in comparison to the vehicle control (24 MN/1000 BNC). Additionally, DNA damages caused by CDW were observed in the comet assay. As a product of LpO, the levels of malondialdehyde (MDA) were significantly enhanced almost in all animals and organs tested after CDW treatment. In livers and serum of rats dose-dependent increases of MDA were observed. The data indicated that extracts from CDW obtained from polluted raw water were able to cause oxidative damages and to induce various biological effects in mammalian cells in vivo and in vitro, i.e. clastogenicity and/or aneugenicity, DNA strand breaks and/or alkali-labile damages. The consistency of the results among the various biological systems and endpoints led to the conclusion that the consumption of chlorinated drinking water obtained from polluted raw water may enhance the body burden with mutagenic and/or carcinogenic substances and therefore, means a potential genetic hazard for human health.
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Format Inist (serveur)
| NO : | PASCAL 02-0309295 INIST |
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| ET : | Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cells |
| AU : | LU (W. Q.); CHEN (X. N.); YUE (F.); JENTER (C.); GMINSKI (R.); LI (X. Y.); XIE (H.); MERSCH-SUNDERMANN (V.) |
| AF : | Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology/Wuhan/Chine (1 aut., 2 aut., 3 aut., 6 aut., 7 aut.); Department of Ecotoxicology and Toxicology, FB VI, University of Trier/54286 Trier/Allemagne (4 aut., 5 aut., 8 aut.) |
| DT : | Publication en série; Niveau analytique |
| SO : | Mutation research. Genetic toxicology and environmental mutagenesis; ISSN 1383-5718; Pays-Bas; Da. 2002; Vol. 513; No. 1-2; Pp. 151-157; Bibl. 20 ref. |
| LA : | Anglais |
| EA : | In the present study, DNA damaging and mutagenic effects of chlorinated drinking water (CDW) extracts obtained from polluted raw water resources were examined in metabolically competent human Hep G2 hepatoma cells using the in vitro micronucleus assay and the single cell gel electrophoresis (SCGE, comet assay). Additionally, the in vivo induction of micronuclei (MN) was studied in polychromatic erythrocytes (PCEs) derived from bone marrow of CDW-treated Wistar rats. Furthermore, we examined the influence of CDW on the lipid peroxidation (LpO) in blood, liver, kidney and testicle of rats. The results demonstrated significant increases of micronucleated PCEs in the bone marrow of rats fed with relatively low CDW doses (33.3 ml/kg body weight per day). Similar effects, i.e. increases of MN frequencies, were found in Hep G2 hepatoma cells after CDW treatment (41 MN/1000 binucleated cells (BNCs) for 167 ml CDW) in comparison to the vehicle control (24 MN/1000 BNC). Additionally, DNA damages caused by CDW were observed in the comet assay. As a product of LpO, the levels of malondialdehyde (MDA) were significantly enhanced almost in all animals and organs tested after CDW treatment. In livers and serum of rats dose-dependent increases of MDA were observed. The data indicated that extracts from CDW obtained from polluted raw water were able to cause oxidative damages and to induce various biological effects in mammalian cells in vivo and in vitro, i.e. clastogenicity and/or aneugenicity, DNA strand breaks and/or alkali-labile damages. The consistency of the results among the various biological systems and endpoints led to the conclusion that the consumption of chlorinated drinking water obtained from polluted raw water may enhance the body burden with mutagenic and/or carcinogenic substances and therefore, means a potential genetic hazard for human health. |
| CC : | 002B03M03; 002A04 |
| FD : | Cellule; DNA; Extrait; Carcinome hépatocellulaire; Tumeur maligne; Micronucléus; Homme; In vitro; In vivo; Lipide; Peroxydation; Eau potable; Rat; Foie pathologie; Appareil digestif pathologie |
| FG : | Rodentia; Mammalia; Vertebrata |
| ED : | Cell; DNA; Extract; Hepatocellular carcinoma; Malignant tumor; Micronucleus; Human; In vitro; In vivo; Lipids; Peroxidation; Drinking water; Rat; Hepatic disease; Digestive diseases |
| EG : | Rodentia; Mammalia; Vertebrata |
| SD : | Célula; DNA; Extracto; Carcinoma hepatocelular; Tumor maligno; Micronúcleo; Hombre; In vitro; In vivo; Lípido; Peroxidación; Agua potable; Rata; Hígado patología; Aparato digestivo patología |
| LO : | INIST-12206H.354000103522440170 |
| ID : | 02-0309295 |
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Lu</name><affiliation><inist:fA14 i1="01"><s1>Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Chen, X N" sort="Chen, X N" uniqKey="Chen X" first="X. N." last="Chen">X. N. Chen</name><affiliation><inist:fA14 i1="01"><s1>Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Yue, F" sort="Yue, F" uniqKey="Yue F" first="F." last="Yue">F. Yue</name><affiliation><inist:fA14 i1="01"><s1>Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Jenter, C" sort="Jenter, C" uniqKey="Jenter C" first="C." last="Jenter">C. Jenter</name><affiliation><inist:fA14 i1="02"><s1>Department of Ecotoxicology and Toxicology, FB VI, University of Trier</s1><s2>54286 Trier</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Gminski, R" sort="Gminski, R" uniqKey="Gminski R" first="R." last="Gminski">R. Gminski</name><affiliation><inist:fA14 i1="02"><s1>Department of Ecotoxicology and Toxicology, FB VI, University of Trier</s1><s2>54286 Trier</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Li, X Y" sort="Li, X Y" uniqKey="Li X" first="X. Y." last="Li">X. Y. Li</name><affiliation><inist:fA14 i1="01"><s1>Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Xie, H" sort="Xie, H" uniqKey="Xie H" first="H." last="Xie">H. Xie</name><affiliation><inist:fA14 i1="01"><s1>Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Mersch Sundermann, V" sort="Mersch Sundermann, V" uniqKey="Mersch Sundermann V" first="V." last="Mersch-Sundermann">V. Mersch-Sundermann</name><affiliation><inist:fA14 i1="02"><s1>Department of Ecotoxicology and Toxicology, FB VI, University of Trier</s1><s2>54286 Trier</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14></affiliation></author></analytic><series><title level="j" type="main">Mutation research. Genetic toxicology and environmental mutagenesis</title><title level="j" type="abbreviated">Mut. res., Genet. toxicol. environ. mutagen.</title><idno type="ISSN">1383-5718</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Mutation research. Genetic toxicology and environmental mutagenesis</title><title level="j" type="abbreviated">Mut. res., Genet. toxicol. environ. mutagen.</title><idno type="ISSN">1383-5718</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell</term><term>DNA</term><term>Digestive diseases</term><term>Drinking water</term><term>Extract</term><term>Hepatic disease</term><term>Hepatocellular carcinoma</term><term>Human</term><term>In vitro</term><term>In vivo</term><term>Lipids</term><term>Malignant tumor</term><term>Micronucleus</term><term>Peroxidation</term><term>Rat</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cellule</term><term>DNA</term><term>Extrait</term><term>Carcinome hépatocellulaire</term><term>Tumeur maligne</term><term>Micronucléus</term><term>Homme</term><term>In vitro</term><term>In vivo</term><term>Lipide</term><term>Peroxydation</term><term>Eau potable</term><term>Rat</term><term>Foie pathologie</term><term>Appareil digestif pathologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present study, DNA damaging and mutagenic effects of chlorinated drinking water (CDW) extracts obtained from polluted raw water resources were examined in metabolically competent human Hep G2 hepatoma cells using the in vitro micronucleus assay and the single cell gel electrophoresis (SCGE, comet assay). Additionally, the in vivo induction of micronuclei (MN) was studied in polychromatic erythrocytes (PCEs) derived from bone marrow of CDW-treated Wistar rats. Furthermore, we examined the influence of CDW on the lipid peroxidation (LpO) in blood, liver, kidney and testicle of rats. The results demonstrated significant increases of micronucleated PCEs in the bone marrow of rats fed with relatively low CDW doses (33.3 ml/kg body weight per day). Similar effects, i.e. increases of MN frequencies, were found in Hep G2 hepatoma cells after CDW treatment (41 MN/1000 binucleated cells (BNCs) for 167 ml CDW) in comparison to the vehicle control (24 MN/1000 BNC). Additionally, DNA damages caused by CDW were observed in the comet assay. As a product of LpO, the levels of malondialdehyde (MDA) were significantly enhanced almost in all animals and organs tested after CDW treatment. In livers and serum of rats dose-dependent increases of MDA were observed. The data indicated that extracts from CDW obtained from polluted raw water were able to cause oxidative damages and to induce various biological effects in mammalian cells in vivo and in vitro, i.e. clastogenicity and/or aneugenicity, DNA strand breaks and/or alkali-labile damages. The consistency of the results among the various biological systems and endpoints led to the conclusion that the consumption of chlorinated drinking water obtained from polluted raw water may enhance the body burden with mutagenic and/or carcinogenic substances and therefore, means a potential genetic hazard for human health.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1383-5718</s0></fA01><fA03 i2="1"><s0>Mut. res., Genet. toxicol. environ. mutagen.</s0></fA03><fA05><s2>513</s2></fA05><fA06><s2>1-2</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cells</s1></fA08><fA11 i1="01" i2="1"><s1>LU (W. Q.)</s1></fA11><fA11 i1="02" i2="1"><s1>CHEN (X. N.)</s1></fA11><fA11 i1="03" i2="1"><s1>YUE (F.)</s1></fA11><fA11 i1="04" i2="1"><s1>JENTER (C.)</s1></fA11><fA11 i1="05" i2="1"><s1>GMINSKI (R.)</s1></fA11><fA11 i1="06" i2="1"><s1>LI (X. Y.)</s1></fA11><fA11 i1="07" i2="1"><s1>XIE (H.)</s1></fA11><fA11 i1="08" i2="1"><s1>MERSCH-SUNDERMANN (V.)</s1></fA11><fA14 i1="01"><s1>Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Ecotoxicology and Toxicology, FB VI, University of Trier</s1><s2>54286 Trier</s2><s3>DEU</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></fA14><fA20><s1>151-157</s1></fA20><fA21><s1>2002</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>12206H</s2><s5>354000103522440170</s5></fA43><fA44><s0>0000</s0><s1>© 2002 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>20 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>02-0309295</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Mutation research. Genetic toxicology and environmental mutagenesis</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>In the present study, DNA damaging and mutagenic effects of chlorinated drinking water (CDW) extracts obtained from polluted raw water resources were examined in metabolically competent human Hep G2 hepatoma cells using the in vitro micronucleus assay and the single cell gel electrophoresis (SCGE, comet assay). Additionally, the in vivo induction of micronuclei (MN) was studied in polychromatic erythrocytes (PCEs) derived from bone marrow of CDW-treated Wistar rats. Furthermore, we examined the influence of CDW on the lipid peroxidation (LpO) in blood, liver, kidney and testicle of rats. The results demonstrated significant increases of micronucleated PCEs in the bone marrow of rats fed with relatively low CDW doses (33.3 ml/kg body weight per day). Similar effects, i.e. increases of MN frequencies, were found in Hep G2 hepatoma cells after CDW treatment (41 MN/1000 binucleated cells (BNCs) for 167 ml CDW) in comparison to the vehicle control (24 MN/1000 BNC). Additionally, DNA damages caused by CDW were observed in the comet assay. As a product of LpO, the levels of malondialdehyde (MDA) were significantly enhanced almost in all animals and organs tested after CDW treatment. In livers and serum of rats dose-dependent increases of MDA were observed. The data indicated that extracts from CDW obtained from polluted raw water were able to cause oxidative damages and to induce various biological effects in mammalian cells in vivo and in vitro, i.e. clastogenicity and/or aneugenicity, DNA strand breaks and/or alkali-labile damages. The consistency of the results among the various biological systems and endpoints led to the conclusion that the consumption of chlorinated drinking water obtained from polluted raw water may enhance the body burden with mutagenic and/or carcinogenic substances and therefore, means a potential genetic hazard for human health.</s0></fC01><fC02 i1="01" i2="X"><s0>002B03M03</s0></fC02><fC02 i1="02" i2="X"><s0>002A04</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Cellule</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Cell</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Célula</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>DNA</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>DNA</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>DNA</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Extrait</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Extract</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Extracto</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Carcinome hépatocellulaire</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Hepatocellular carcinoma</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Carcinoma hepatocelular</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Tumeur maligne</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Malignant tumor</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Tumor maligno</s0><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Micronucléus</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Micronucleus</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Micronúcleo</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Homme</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Human</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Hombre</s0><s5>07</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>In vitro</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>In vitro</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>In vitro</s0><s5>08</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>In vivo</s0><s5>09</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>In vivo</s0><s5>09</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>In vivo</s0><s5>09</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Lipide</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Lipids</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Lípido</s0><s5>10</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Peroxydation</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Peroxidation</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Peroxidación</s0><s5>11</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Eau potable</s0><s5>12</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Drinking water</s0><s5>12</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Agua potable</s0><s5>12</s5></fC03><fC03 i1="13" i2="X" l="FRE"><s0>Rat</s0><s5>13</s5></fC03><fC03 i1="13" i2="X" l="ENG"><s0>Rat</s0><s5>13</s5></fC03><fC03 i1="13" i2="X" l="SPA"><s0>Rata</s0><s5>13</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>Foie pathologie</s0><s5>47</s5></fC03><fC03 i1="14" i2="X" l="ENG"><s0>Hepatic disease</s0><s5>47</s5></fC03><fC03 i1="14" i2="X" l="SPA"><s0>Hígado patología</s0><s5>47</s5></fC03><fC03 i1="15" i2="X" l="FRE"><s0>Appareil digestif pathologie</s0><s5>48</s5></fC03><fC03 i1="15" i2="X" l="ENG"><s0>Digestive diseases</s0><s5>48</s5></fC03><fC03 i1="15" i2="X" l="SPA"><s0>Aparato digestivo patología</s0><s5>48</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Rodentia</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Rodentia</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Rodentia</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Vertebrata</s0><s2>NS</s2></fC07><fN21><s1>175</s1></fN21><fN82><s1>DST</s1></fN82></pA></standard><server><NO>PASCAL 02-0309295 INIST</NO><ET>Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cells</ET><AU>LU (W. Q.); CHEN (X. N.); YUE (F.); JENTER (C.); GMINSKI (R.); LI (X. Y.); XIE (H.); MERSCH-SUNDERMANN (V.)</AU><AF>Department of Environmental Health, Tongji Medical College of Huazhong University of Science and Technology/Wuhan/Chine (1 aut., 2 aut., 3 aut., 6 aut., 7 aut.); Department of Ecotoxicology and Toxicology, FB VI, University of Trier/54286 Trier/Allemagne (4 aut., 5 aut., 8 aut.)</AF><DT>Publication en série; Niveau analytique</DT><SO>Mutation research. Genetic toxicology and environmental mutagenesis; ISSN 1383-5718; Pays-Bas; Da. 2002; Vol. 513; No. 1-2; Pp. 151-157; Bibl. 20 ref.</SO><LA>Anglais</LA><EA>In the present study, DNA damaging and mutagenic effects of chlorinated drinking water (CDW) extracts obtained from polluted raw water resources were examined in metabolically competent human Hep G2 hepatoma cells using the in vitro micronucleus assay and the single cell gel electrophoresis (SCGE, comet assay). Additionally, the in vivo induction of micronuclei (MN) was studied in polychromatic erythrocytes (PCEs) derived from bone marrow of CDW-treated Wistar rats. Furthermore, we examined the influence of CDW on the lipid peroxidation (LpO) in blood, liver, kidney and testicle of rats. The results demonstrated significant increases of micronucleated PCEs in the bone marrow of rats fed with relatively low CDW doses (33.3 ml/kg body weight per day). Similar effects, i.e. increases of MN frequencies, were found in Hep G2 hepatoma cells after CDW treatment (41 MN/1000 binucleated cells (BNCs) for 167 ml CDW) in comparison to the vehicle control (24 MN/1000 BNC). Additionally, DNA damages caused by CDW were observed in the comet assay. As a product of LpO, the levels of malondialdehyde (MDA) were significantly enhanced almost in all animals and organs tested after CDW treatment. In livers and serum of rats dose-dependent increases of MDA were observed. The data indicated that extracts from CDW obtained from polluted raw water were able to cause oxidative damages and to induce various biological effects in mammalian cells in vivo and in vitro, i.e. clastogenicity and/or aneugenicity, DNA strand breaks and/or alkali-labile damages. The consistency of the results among the various biological systems and endpoints led to the conclusion that the consumption of chlorinated drinking water obtained from polluted raw water may enhance the body burden with mutagenic and/or carcinogenic substances and therefore, means a potential genetic hazard for human health.</EA><CC>002B03M03; 002A04</CC><FD>Cellule; DNA; Extrait; Carcinome hépatocellulaire; Tumeur maligne; Micronucléus; Homme; In vitro; In vivo; Lipide; Peroxydation; Eau potable; Rat; Foie pathologie; Appareil digestif pathologie</FD><FG>Rodentia; Mammalia; Vertebrata</FG><ED>Cell; DNA; Extract; Hepatocellular carcinoma; Malignant tumor; Micronucleus; Human; In vitro; In vivo; Lipids; Peroxidation; Drinking water; Rat; Hepatic disease; Digestive diseases</ED><EG>Rodentia; Mammalia; Vertebrata</EG><SD>Célula; DNA; Extracto; Carcinoma hepatocelular; Tumor maligno; Micronúcleo; Hombre; In vitro; In vivo; Lípido; Peroxidación; Agua potable; Rata; Hígado patología; Aparato digestivo patología</SD><LO>INIST-12206H.354000103522440170</LO><ID>02-0309295</ID></server></inist></record>Pour manipuler ce document sous Unix (Dilib)
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