The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus
Identifieur interne : 003295 ( Main/Exploration ); précédent : 003294; suivant : 003296The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus
Auteurs : V. R. Winrow [Royaume-Uni] ; J. Norton [Royaume-Uni] ; J. D. Perry [Royaume-Uni] ; E. J. Holborow [Royaume-Uni] ; H. E. Stierle [Allemagne]Source :
- Journal of Immunological Methods [ 0022-1759 ] ; 1986.
Abstract
Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.
Url:
DOI: 10.1016/0022-1759(87)90463-7
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000B62
- to stream Istex, to step Curation: 000A95
- to stream Istex, to step Checkpoint: 001712
- to stream Main, to step Merge: 003941
- to stream Main, to step Curation: 003295
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title><author><name sortKey="Winrow, V R" sort="Winrow, V R" uniqKey="Winrow V" first="V. R." last="Winrow">V. R. Winrow</name></author><author><name sortKey="Norton, J" sort="Norton, J" uniqKey="Norton J" first="J." last="Norton">J. Norton</name></author><author><name sortKey="Perry, J D" sort="Perry, J D" uniqKey="Perry J" first="J. D." last="Perry">J. D. Perry</name></author><author><name sortKey="Holborow, E J" sort="Holborow, E J" uniqKey="Holborow E" first="E. J." last="Holborow">E. J. Holborow</name></author><author><name sortKey="Stierle, H E" sort="Stierle, H E" uniqKey="Stierle H" first="H. E." last="Stierle">H. E. Stierle</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:63608A87A80115AE4BA983D969449C4D319ADDE7</idno><date when="1986" year="1986">1986</date><idno type="doi">10.1016/0022-1759(87)90463-7</idno><idno type="url">https://api.istex.fr/document/63608A87A80115AE4BA983D969449C4D319ADDE7/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B62</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B62</idno><idno type="wicri:Area/Istex/Curation">000A95</idno><idno type="wicri:Area/Istex/Checkpoint">001712</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001712</idno><idno type="wicri:doubleKey">0022-1759:1986:Winrow V:the:effects:of</idno><idno type="wicri:Area/Main/Merge">003941</idno><idno type="wicri:Area/Main/Curation">003295</idno><idno type="wicri:Area/Main/Exploration">003295</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title><author><name sortKey="Winrow, V R" sort="Winrow, V R" uniqKey="Winrow V" first="V. R." last="Winrow">V. R. Winrow</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD</wicri:regionArea><wicri:noRegion>London E1 2AD</wicri:noRegion></affiliation></author><author><name sortKey="Norton, J" sort="Norton, J" uniqKey="Norton J" first="J." last="Norton">J. Norton</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD</wicri:regionArea><wicri:noRegion>London E1 2AD</wicri:noRegion></affiliation></author><author><name sortKey="Perry, J D" sort="Perry, J D" uniqKey="Perry J" first="J. D." last="Perry">J. D. Perry</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD</wicri:regionArea><wicri:noRegion>London E1 2AD</wicri:noRegion></affiliation></author><author><name sortKey="Holborow, E J" sort="Holborow, E J" uniqKey="Holborow E" first="E. J." last="Holborow">E. J. Holborow</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD</wicri:regionArea><wicri:noRegion>London E1 2AD</wicri:noRegion></affiliation></author><author><name sortKey="Stierle, H E" sort="Stierle, H E" uniqKey="Stierle H" first="H. E." last="Stierle">H. E. Stierle</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Allemagne</country><wicri:regionArea>II Med. Abtl., Krankenhaus der Barmherzigen Brüder, D-5500 Trier</wicri:regionArea><wicri:noRegion>D-5500 Trier</wicri:noRegion><wicri:noRegion>D-5500 Trier</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986">1986</date><biblScope unit="volume">97</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="227">227</biblScope></imprint><idno type="ISSN">0022-1759</idno></series><idno type="istex">63608A87A80115AE4BA983D969449C4D319ADDE7</idno><idno type="DOI">10.1016/0022-1759(87)90463-7</idno><idno type="PII">0022-1759(87)90463-7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Winrow, V R" sort="Winrow, V R" uniqKey="Winrow V" first="V. R." last="Winrow">V. R. Winrow</name></noRegion><name sortKey="Holborow, E J" sort="Holborow, E J" uniqKey="Holborow E" first="E. J." last="Holborow">E. J. Holborow</name><name sortKey="Norton, J" sort="Norton, J" uniqKey="Norton J" first="J." last="Norton">J. Norton</name><name sortKey="Perry, J D" sort="Perry, J D" uniqKey="Perry J" first="J. D." last="Perry">J. D. Perry</name></country><country name="Allemagne"><noRegion><name sortKey="Stierle, H E" sort="Stierle, H E" uniqKey="Stierle H" first="H. E." last="Stierle">H. E. Stierle</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Rhénanie/explor/UnivTrevesV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003295 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003295 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Rhénanie
|area= UnivTrevesV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:63608A87A80115AE4BA983D969449C4D319ADDE7
|texte= The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus
}}
| This area was generated with Dilib version V0.6.31. |  |