The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus
Identifieur interne : 000B62 ( Istex/Corpus ); précédent : 000B61; suivant : 000B63The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus
Auteurs : V. R. Winrow ; J. Norton ; J. D. Perry ; E. J. Holborow ; H. E. StierleSource :
- Journal of Immunological Methods [ 0022-1759 ] ; 1986.
Abstract
Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.
Url:
DOI: 10.1016/0022-1759(87)90463-7
Links to Exploration step
ISTEX:63608A87A80115AE4BA983D969449C4D319ADDE7Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title><author><name sortKey="Winrow, V R" sort="Winrow, V R" uniqKey="Winrow V" first="V. R." last="Winrow">V. R. Winrow</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Norton, J" sort="Norton, J" uniqKey="Norton J" first="J." last="Norton">J. Norton</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Perry, J D" sort="Perry, J D" uniqKey="Perry J" first="J. D." last="Perry">J. D. Perry</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Holborow, E J" sort="Holborow, E J" uniqKey="Holborow E" first="E. J." last="Holborow">E. J. Holborow</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Stierle, H E" sort="Stierle, H E" uniqKey="Stierle H" first="H. E." last="Stierle">H. E. Stierle</name><affiliation><mods:affiliation>II Med. Abtl., Krankenhaus der Barmherzigen Brüder, D-5500 Trier, F.R.G.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:63608A87A80115AE4BA983D969449C4D319ADDE7</idno><date when="1986" year="1986">1986</date><idno type="doi">10.1016/0022-1759(87)90463-7</idno><idno type="url">https://api.istex.fr/document/63608A87A80115AE4BA983D969449C4D319ADDE7/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B62</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B62</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title><author><name sortKey="Winrow, V R" sort="Winrow, V R" uniqKey="Winrow V" first="V. R." last="Winrow">V. R. Winrow</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Norton, J" sort="Norton, J" uniqKey="Norton J" first="J." last="Norton">J. Norton</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Perry, J D" sort="Perry, J D" uniqKey="Perry J" first="J. D." last="Perry">J. D. Perry</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Holborow, E J" sort="Holborow, E J" uniqKey="Holborow E" first="E. J." last="Holborow">E. J. Holborow</name><affiliation><mods:affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Stierle, H E" sort="Stierle, H E" uniqKey="Stierle H" first="H. E." last="Stierle">H. E. Stierle</name><affiliation><mods:affiliation>II Med. Abtl., Krankenhaus der Barmherzigen Brüder, D-5500 Trier, F.R.G.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986">1986</date><biblScope unit="volume">97</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="227">227</biblScope></imprint><idno type="ISSN">0022-1759</idno></series><idno type="istex">63608A87A80115AE4BA983D969449C4D319ADDE7</idno><idno type="DOI">10.1016/0022-1759(87)90463-7</idno><idno type="PII">0022-1759(87)90463-7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>V.R. Winrow</name><affiliations><json:string>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</json:string></affiliations></json:item><json:item><name>J. Norton</name><affiliations><json:string>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</json:string></affiliations></json:item><json:item><name>J.D. Perry</name><affiliations><json:string>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</json:string></affiliations></json:item><json:item><name>E.J. Holborow</name><affiliations><json:string>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</json:string></affiliations></json:item><json:item><name>H.E. Stierle</name><affiliations><json:string>II Med. Abtl., Krankenhaus der Barmherzigen Brüder, D-5500 Trier, F.R.G.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Rheumatoid arthritis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Monocyte</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Epstein-Barr virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Null cell</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.</abstract><qualityIndicators><score>4.646</score><pdfVersion>1.2</pdfVersion><pdfPageSize>526 x 742 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>938</abstractCharCount><pdfWordCount>3158</pdfWordCount><pdfCharCount>19558</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>124</abstractWordCount></qualityIndicators><title>The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title><pii><json:string>0022-1759(87)90463-7</json:string></pii><refBibs><json:item><host><volume>147</volume><pages><first>1018</first></pages><author></author><title>J. Exp. Med.</title></host></json:item><json:item><host><volume>23</volume><pages><first>626</first></pages><author></author><title>Arthritis Rheum.</title></host></json:item><json:item><host><volume>21</volume><pages><first>77</first></pages><issue>suppl. 97</issue><author></author><title>Scand. J. Clin. Lab. Invest.</title></host></json:item><json:item><host><volume>124</volume><pages><first>565</first></pages><author></author><title>J. Immunol.</title></host></json:item><json:item><host><volume>4</volume><pages><first>19</first></pages><author></author><title>Rheumatol. Int.</title></host></json:item><json:item><host><volume>53</volume><pages><first>61</first></pages><author></author><title>Med. Biol.</title></host></json:item><json:item><host><volume>24</volume><pages><first>755</first></pages><author></author><title>Arthritis Rheum.</title></host></json:item><json:item><host><volume>44</volume><pages><first>8</first></pages><author></author><title>Ann. Rheum. Dis.</title></host></json:item><json:item><host><volume>67</volume><pages><first>681</first></pages><author></author><title>J. Clin. Invest.</title></host></json:item><json:item><host><volume>6</volume><pages><first>231</first></pages><author></author><title>Rheumatology</title></host></json:item><json:item><host><volume>2</volume><pages><first>155</first></pages><author></author><title>Rheumatol. Int.</title></host></json:item><json:item><host><pages><first>1</first></pages><author></author><title>Immunological Aspects of Rheumatology</title></host></json:item><json:item><host><volume>157</volume><pages><first>173</first></pages><author></author><title>J. Exp. Med.</title></host></json:item><json:item><host><volume>131</volume><pages><first>768</first></pages><author></author><title>J. Immunol.</title></host></json:item><json:item><host><volume>5</volume><pages><first>148</first></pages><author></author><title>Immunol. Today</title></host></json:item><json:item><host><volume>28</volume><pages><first>961</first></pages><author></author><title>Arthritis Rheum.</title></host></json:item><json:item><host><volume>5</volume><pages><first>131</first></pages><author></author><title>J. Immunol. Methods</title></host></json:item><json:item><host><volume>134</volume><pages><first>2287</first></pages><author></author><title>J. Immunol.</title></host></json:item><json:item><host><volume>i</volume><pages><first>447</first></pages><author></author><title>Lancet</title></host></json:item><json:item><host><volume>9</volume><pages><first>387</first></pages><author></author><title>Scand. J. Immunol.</title></host></json:item><json:item><host><volume>28</volume><pages><first>971</first></pages><author></author><title>Arthritis Rheum.</title></host></json:item><json:item><host><volume>2</volume><pages><first>175</first></pages><author></author><title>Rheumatol. Int.</title></host></json:item><json:item><host><volume>148</volume><pages><first>1429</first></pages><author></author><title>J. Exp. Med.</title></host></json:item><json:item><host><volume>51</volume><pages><first>337</first></pages><author></author><title>Immunol. Rev.</title></host></json:item><json:item><host><volume>3</volume><pages><first>7</first></pages><author></author><title>Rheumatol. Int.</title></host></json:item><json:item><host><pages><first>185</first></pages><author></author><title>The Epstein-Barr Virus</title></host></json:item><json:item><host><volume>126</volume><pages><first>829</first></pages><author></author><title>J. Immunol.</title></host></json:item><json:item><host><volume>305</volume><pages><first>1238</first></pages><author></author><title>N. Engl. J. Med.</title></host></json:item><json:item><host><volume>24</volume><pages><first>8</first></pages><author></author><title>Clin. Immunol. Immunopathol.</title></host></json:item><json:item><host><volume>6</volume><pages><first>381</first></pages><author></author><title>J. Rheumatol.</title></host></json:item><json:item><host><volume>2</volume><pages><first>102</first></pages><author></author><title>Clin. Rheumatol.</title></host></json:item><json:item><host><volume>44</volume><pages><first>2</first></pages><author></author><title>Ann. Rheum. Dis.</title></host></json:item><json:item><host><volume>41</volume><pages><first>279</first></pages><author></author><title>J. Immunol. Methods</title></host></json:item></refBibs><genre><json:string>research-article</json:string></genre><host><volume>97</volume><pii><json:string>S0022-1759(00)X0485-1</json:string></pii><pages><last>227</last><first>221</first></pages><issn><json:string>0022-1759</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Journal of Immunological Methods</title><publicationDate>1987</publicationDate></host><categories><wos><json:string>science</json:string><json:string>immunology</json:string><json:string>biochemical research methods</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>immunology</json:string></scienceMetrix></categories><publicationDate>1986</publicationDate><copyrightDate>1986</copyrightDate><doi><json:string>10.1016/0022-1759(87)90463-7</json:string></doi><id>63608A87A80115AE4BA983D969449C4D319ADDE7</id><score>0.20479405</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/63608A87A80115AE4BA983D969449C4D319ADDE7/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/63608A87A80115AE4BA983D969449C4D319ADDE7/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/63608A87A80115AE4BA983D969449C4D319ADDE7/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1986</date></publicationStmt><notesStmt><note type="content">Section title: Research report</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title><author xml:id="author-1"><persName><forename type="first">V.R.</forename><surname>Winrow</surname></persName><note type="correspondence"><p>Correspondence to: V.R. Winrow, ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</p></note><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation></author><author xml:id="author-2"><persName><forename type="first">J.</forename><surname>Norton</surname></persName><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation></author><author xml:id="author-3"><persName><forename type="first">J.D.</forename><surname>Perry</surname></persName><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation></author><author xml:id="author-4"><persName><forename type="first">E.J.</forename><surname>Holborow</surname></persName><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation></author><author xml:id="author-5"><persName><forename type="first">H.E.</forename><surname>Stierle</surname></persName><affiliation>II Med. Abtl., Krankenhaus der Barmherzigen Brüder, D-5500 Trier, F.R.G.</affiliation></author></analytic><monogr><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="pISSN">0022-1759</idno><idno type="PII">S0022-1759(00)X0485-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986"></date><biblScope unit="volume">97</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="227">227</biblScope></imprint></monogr><idno type="istex">63608A87A80115AE4BA983D969449C4D319ADDE7</idno><idno type="DOI">10.1016/0022-1759(87)90463-7</idno><idno type="PII">0022-1759(87)90463-7</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1986</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Rheumatoid arthritis</term></item><item><term>Monocyte</term></item><item><term>Epstein-Barr virus</term></item><item><term>Null cell</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1986">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/63608A87A80115AE4BA983D969449C4D319ADDE7/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JIM</jid><aid>87904637</aid><ce:pii>0022-1759(87)90463-7</ce:pii><ce:doi>10.1016/0022-1759(87)90463-7</ce:doi><ce:copyright type="unknown" year="1986"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Research report</ce:textfn></ce:dochead><ce:title>The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</ce:title><ce:author-group><ce:author><ce:given-name>V.R.</ce:given-name><ce:surname>Winrow</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Norton</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.D.</ce:given-name><ce:surname>Perry</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.J.</ce:given-name><ce:surname>Holborow</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>H.E.</ce:given-name><ce:surname>Stierle</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>1</ce:label><ce:textfn>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>2</ce:label><ce:textfn>II Med. Abtl., Krankenhaus der Barmherzigen Brüder, D-5500 Trier, F.R.G.</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: V.R. Winrow, ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="29" month="9" year="1986"></ce:date-received><ce:date-accepted day="11" month="11" year="1986"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.</ce:simple-para><ce:simple-para>The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Rheumatoid arthritis</ce:text></ce:keyword><ce:keyword><ce:text>Monocyte</ce:text></ce:keyword><ce:keyword><ce:text>Epstein-Barr virus</ce:text></ce:keyword><ce:keyword><ce:text>Null cell</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus</title></titleInfo><name type="personal"><namePart type="given">V.R.</namePart><namePart type="family">Winrow</namePart><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation><description>Correspondence to: V.R. Winrow, ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Norton</namePart><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.D.</namePart><namePart type="family">Perry</namePart><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.J.</namePart><namePart type="family">Holborow</namePart><affiliation>ARC Bone and Joint Research Unit, The London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, U.K.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.E.</namePart><namePart type="family">Stierle</namePart><affiliation>II Med. Abtl., Krankenhaus der Barmherzigen Brüder, D-5500 Trier, F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1986</dateIssued><copyrightDate encoding="w3cdtf">1986</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Rheumatoid peripheral blood mononuclear cells show an increased responsiveness to superinfection with Epstein-Barr virus (EBV). We have investigated the role of adherent cells in this hyperresponsiveness using two different methods of adherent cell depletion. Depletion of adherent cells from both rheumatoid and normal mononuclear cells, using either dextran bead columns or plastic petri dishes, produced inconsistent changes in the response of autologous non-adherent cells to EBV. The addition of supernatants of cultured rheumatoid adherent cells also produced an inconsistent change in response although normal adherent cell supernatants increased the responsiveness of autologous non-adherent cells to EBV.The inconsistencies observed are discussed with respect to adherent cell subpopulations present in rheumatoid and normal peripheral blood mononuclear cell preparations when using recognized methods of adherent cell depletion.</abstract><note type="content">Section title: Research report</note><subject><genre>Keywords</genre><topic>Rheumatoid arthritis</topic><topic>Monocyte</topic><topic>Epstein-Barr virus</topic><topic>Null cell</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Immunological Methods</title></titleInfo><titleInfo type="abbreviated"><title>JIM</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19870312</dateIssued></originInfo><identifier type="ISSN">0022-1759</identifier><identifier type="PII">S0022-1759(00)X0485-1</identifier><part><date>19870312</date><detail type="volume"><number>97</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>153</start><end>300</end></extent><extent unit="pages"><start>221</start><end>227</end></extent></part></relatedItem><identifier type="istex">63608A87A80115AE4BA983D969449C4D319ADDE7</identifier><identifier type="DOI">10.1016/0022-1759(87)90463-7</identifier><identifier type="PII">0022-1759(87)90463-7</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Rhénanie/explor/UnivTrevesV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000B62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Rhénanie
|area= UnivTrevesV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:63608A87A80115AE4BA983D969449C4D319ADDE7
|texte= The effects of adherent cells on measurement of the hyper-responsiveness of rheumatoid B lymphocytes to Epstein-Barr virus
}}
| This area was generated with Dilib version V0.6.31. |  |