Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions
Identifieur interne : 001765 ( Istex/Corpus ); précédent : 001764; suivant : 001766Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions
Auteurs : Axel Meyer ; Holger Fischer ; Yakov Kuzyakov ; Klaus FischerSource :
- Journal of Plant Nutrition and Soil Science [ 1436-8730 ] ; 2008-12.
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Abstract
In spite of their low concentrations in soil solutions, low–molecular weight organic substances (LMWOS) such as amino acids, sugars, and uronic acids play a major role in the cycles of C and N in soil. With respect to their low concentrations and to possible matrix interferences, their analysis in soil leachates is a challenging task. We established two HPLC (high‐performance liquid chromatography) methods for the parallel determination of amino acids and carbohydrates in soil leachates. The pre‐column derivatization of amino acids with an o‐phthaldialdehyde (OPA) mercaptoethanol solution yields quantitation limits between 0.03 and 0.44 µmol L–1 and SD values of <8.3% (n = 9). High‐performance anion‐exchange chromatography (HPAEC) on a Dionex CarboPac PA 20 column with a NaOH acetate gradient combined with pulsed amperometric detection (PAD) was used for the determination of carbohydrates. The calibration curves obtained for 11 carbohydrates showed excellent linearity over the concentration range from 0.02 to 50.0 mg L–1. Recovery studies revealed good results for all analytes (89%–108%). Interferences from Hg(II) salts and chloroform used for stabilization of the leachates did not occur with both chromatographic methods. The optimized method was successfully used for quantitative determinations of amino acids and carbohydrates in soil leachates.
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DOI: 10.1002/jpln.200700235
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions</title><author><name sortKey="Meyer, Axel" sort="Meyer, Axel" uniqKey="Meyer A" first="Axel" last="Meyer">Axel Meyer</name><affiliation><mods:affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: meyerax@uni‐trier.de</mods:affiliation></affiliation></author><author><name sortKey="Fischer, Holger" sort="Fischer, Holger" uniqKey="Fischer H" first="Holger" last="Fischer">Holger Fischer</name><affiliation><mods:affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil‐Wolff‐Str. 27, 70593 Stuttgart, Germany</mods:affiliation></affiliation></author><author><name sortKey="Kuzyakov, Yakov" sort="Kuzyakov, Yakov" uniqKey="Kuzyakov Y" first="Yakov" last="Kuzyakov">Yakov Kuzyakov</name><affiliation><mods:affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</mods:affiliation></affiliation></author><author><name sortKey="Fischer, Klaus" sort="Fischer, Klaus" uniqKey="Fischer K" first="Klaus" last="Fischer">Klaus Fischer</name><affiliation><mods:affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2F95600B2AA916EC9605741F49D343E9A5875B2A</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1002/jpln.200700235</idno><idno type="url">https://api.istex.fr/document/2F95600B2AA916EC9605741F49D343E9A5875B2A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001765</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001765</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions</title><author><name sortKey="Meyer, Axel" sort="Meyer, Axel" uniqKey="Meyer A" first="Axel" last="Meyer">Axel Meyer</name><affiliation><mods:affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: meyerax@uni‐trier.de</mods:affiliation></affiliation></author><author><name sortKey="Fischer, Holger" sort="Fischer, Holger" uniqKey="Fischer H" first="Holger" last="Fischer">Holger Fischer</name><affiliation><mods:affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil‐Wolff‐Str. 27, 70593 Stuttgart, Germany</mods:affiliation></affiliation></author><author><name sortKey="Kuzyakov, Yakov" sort="Kuzyakov, Yakov" uniqKey="Kuzyakov Y" first="Yakov" last="Kuzyakov">Yakov Kuzyakov</name><affiliation><mods:affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</mods:affiliation></affiliation></author><author><name sortKey="Fischer, Klaus" sort="Fischer, Klaus" uniqKey="Fischer K" first="Klaus" last="Fischer">Klaus Fischer</name><affiliation><mods:affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Plant Nutrition and Soil Science</title><title level="j" type="abbrev">Z. Pflanzenernähr. Bodenk.</title><idno type="ISSN">1436-8730</idno><idno type="eISSN">1522-2624</idno><imprint><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2008-12">2008-12</date><biblScope unit="volume">171</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="917">917</biblScope><biblScope unit="page" to="926">926</biblScope></imprint><idno type="ISSN">1436-8730</idno></series><idno type="istex">2F95600B2AA916EC9605741F49D343E9A5875B2A</idno><idno type="DOI">10.1002/jpln.200700235</idno><idno type="ArticleID">JPLN200700235</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1436-8730</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>HPAEC‐PAD</term><term>amino acids</term><term>amino sugars</term><term>carbohydrates</term><term>low–molecular weight organic substances</term><term>monosaccharides</term><term>soil leachates</term><term>uronic acids</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In spite of their low concentrations in soil solutions, low–molecular weight organic substances (LMWOS) such as amino acids, sugars, and uronic acids play a major role in the cycles of C and N in soil. With respect to their low concentrations and to possible matrix interferences, their analysis in soil leachates is a challenging task. We established two HPLC (high‐performance liquid chromatography) methods for the parallel determination of amino acids and carbohydrates in soil leachates. The pre‐column derivatization of amino acids with an o‐phthaldialdehyde (OPA) mercaptoethanol solution yields quantitation limits between 0.03 and 0.44 µmol L–1 and SD values of <8.3% (n = 9). High‐performance anion‐exchange chromatography (HPAEC) on a Dionex CarboPac PA 20 column with a NaOH acetate gradient combined with pulsed amperometric detection (PAD) was used for the determination of carbohydrates. The calibration curves obtained for 11 carbohydrates showed excellent linearity over the concentration range from 0.02 to 50.0 mg L–1. Recovery studies revealed good results for all analytes (89%–108%). Interferences from Hg(II) salts and chloroform used for stabilization of the leachates did not occur with both chromatographic methods. The optimized method was successfully used for quantitative determinations of amino acids and carbohydrates in soil leachates.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Axel Meyer</name><affiliations><json:string>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</json:string><json:string>E-mail: meyerax@uni‐trier.de</json:string></affiliations></json:item><json:item><name>Holger Fischer</name><affiliations><json:string>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</json:string><json:string>Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil‐Wolff‐Str. 27, 70593 Stuttgart, Germany</json:string></affiliations></json:item><json:item><name>Yakov Kuzyakov</name><affiliations><json:string>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</json:string></affiliations></json:item><json:item><name>Klaus Fischer</name><affiliations><json:string>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>low–molecular weight organic substances</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>amino acids</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>soil leachates</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HPAEC‐PAD</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>carbohydrates</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>monosaccharides</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>amino sugars</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>uronic acids</value></json:item></subject><articleId><json:string>JPLN200700235</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>In spite of their low concentrations in soil solutions, low–molecular weight organic substances (LMWOS) such as amino acids, sugars, and uronic acids play a major role in the cycles of C and N in soil. With respect to their low concentrations and to possible matrix interferences, their analysis in soil leachates is a challenging task. We established two HPLC (high‐performance liquid chromatography) methods for the parallel determination of amino acids and carbohydrates in soil leachates. The pre‐column derivatization of amino acids with an o‐phthaldialdehyde (OPA) mercaptoethanol solution yields quantitation limits between 0.03 and 0.44 µmol L–1 and SD values of >8.3% (n = 9). High‐performance anion‐exchange chromatography (HPAEC) on a Dionex CarboPac PA 20 column with a NaOH acetate gradient combined with pulsed amperometric detection (PAD) was used for the determination of carbohydrates. The calibration curves obtained for 11 carbohydrates showed excellent linearity over the concentration range from 0.02 to 50.0 mg L–1. Recovery studies revealed good results for all analytes (89%–108%). Interferences from Hg(II) salts and chloroform used for stabilization of the leachates did not occur with both chromatographic methods. The optimized method was successfully used for quantitative determinations of amino acids and carbohydrates in soil leachates.</abstract><qualityIndicators><score>7.352</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595 x 794 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1368</abstractCharCount><pdfWordCount>5030</pdfWordCount><pdfCharCount>34511</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>196</abstractWordCount></qualityIndicators><title>Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions</title><refBibs><json:item><author><json:item><name>G. Bachmann</name></json:item><json:item><name>H. 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KGaA, Weinheim</p></availability><date>2008</date></publicationStmt><notesStmt><note>Deutsche Forschungsgemeinschaft</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions</title><author xml:id="author-1"><persName><forename type="first">Axel</forename><surname>Meyer</surname></persName><email>meyerax@uni‐trier.de</email><affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</affiliation></author><author xml:id="author-2"><persName><forename type="first">Holger</forename><surname>Fischer</surname></persName><affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</affiliation><affiliation>Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil‐Wolff‐Str. 27, 70593 Stuttgart, Germany</affiliation></author><author xml:id="author-3"><persName><forename type="first">Yakov</forename><surname>Kuzyakov</surname></persName><affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</affiliation></author><author xml:id="author-4"><persName><forename type="first">Klaus</forename><surname>Fischer</surname></persName><affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</affiliation></author></analytic><monogr><title level="j">Journal of Plant Nutrition and Soil Science</title><title level="j" type="abbrev">Z. Pflanzenernähr. Bodenk.</title><idno type="pISSN">1436-8730</idno><idno type="eISSN">1522-2624</idno><idno type="DOI">10.1002/(ISSN)1522-2624</idno><imprint><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2008-12"></date><biblScope unit="volume">171</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="917">917</biblScope><biblScope unit="page" to="926">926</biblScope></imprint></monogr><idno type="istex">2F95600B2AA916EC9605741F49D343E9A5875B2A</idno><idno type="DOI">10.1002/jpln.200700235</idno><idno type="ArticleID">JPLN200700235</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2008</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>In spite of their low concentrations in soil solutions, low–molecular weight organic substances (LMWOS) such as amino acids, sugars, and uronic acids play a major role in the cycles of C and N in soil. With respect to their low concentrations and to possible matrix interferences, their analysis in soil leachates is a challenging task. We established two HPLC (high‐performance liquid chromatography) methods for the parallel determination of amino acids and carbohydrates in soil leachates. The pre‐column derivatization of amino acids with an o‐phthaldialdehyde (OPA) mercaptoethanol solution yields quantitation limits between 0.03 and 0.44 µmol L–1 and SD values of <8.3% (n = 9). High‐performance anion‐exchange chromatography (HPAEC) on a Dionex CarboPac PA 20 column with a NaOH acetate gradient combined with pulsed amperometric detection (PAD) was used for the determination of carbohydrates. The calibration curves obtained for 11 carbohydrates showed excellent linearity over the concentration range from 0.02 to 50.0 mg L–1. Recovery studies revealed good results for all analytes (89%–108%). Interferences from Hg(II) salts and chloroform used for stabilization of the leachates did not occur with both chromatographic methods. The optimized method was successfully used for quantitative determinations of amino acids and carbohydrates in soil leachates.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>low–molecular weight organic substances</term></item><item><term>amino acids</term></item><item><term>soil leachates</term></item><item><term>HPAEC‐PAD</term></item><item><term>carbohydrates</term></item><item><term>monosaccharides</term></item><item><term>amino sugars</term></item><item><term>uronic acids</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Regular Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-01-02">Registration</change><change when="2008-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/2F95600B2AA916EC9605741F49D343E9A5875B2A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>WILEY‐VCH Verlag</publisherName><publisherLoc>Weinheim</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1522-2624</doi><issn type="print">1436-8730</issn><issn type="electronic">1522-2624</issn><idGroup><id type="product" value="JPLN"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE ZEITSCHRIFT FUER PFLANZENERNAEHRUNG UND BODENKUNDE">Journal of Plant Nutrition and Soil Science</title><title type="tocForm">Journal of Plant Nutrition and Soil Science / Zeitschrift für Pflanzenernährung und Bodenkunde</title><title type="short">Z. 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With respect to their low concentrations and to possible matrix interferences, their analysis in soil leachates is a challenging task. We established two HPLC (high‐performance liquid chromatography) methods for the parallel determination of amino acids and carbohydrates in soil leachates. The pre‐column derivatization of amino acids with an o‐phthaldialdehyde (OPA) mercaptoethanol solution yields quantitation limits between 0.03 and 0.44 µmol L<sup>–1</sup> and S<sub>D </sub>values of <8.3% (<i>n</i> = 9). High‐performance anion‐exchange chromatography (HPAEC) on a Dionex CarboPac PA 20 column with a NaOH acetate gradient combined with pulsed amperometric detection (PAD) was used for the determination of carbohydrates. The calibration curves obtained for 11 carbohydrates showed excellent linearity over the concentration range from 0.02 to 50.0 mg L<sup>–1</sup>. Recovery studies revealed good results for all analytes (89%–108%). Interferences from Hg(II) salts and chloroform used for stabilization of the leachates did not occur with both chromatographic methods. The optimized method was successfully used for quantitative determinations of amino acids and carbohydrates in soil leachates.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions</title></titleInfo><name type="personal"><namePart type="given">Axel</namePart><namePart type="family">Meyer</namePart><affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</affiliation><affiliation>E-mail: meyerax@uni‐trier.de</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Holger</namePart><namePart type="family">Fischer</namePart><affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</affiliation><affiliation>Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil‐Wolff‐Str. 27, 70593 Stuttgart, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yakov</namePart><namePart type="family">Kuzyakov</namePart><affiliation>Department of Agroecosystem Research, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Klaus</namePart><namePart type="family">Fischer</namePart><affiliation>University of Trier, FB VI Geography/Geosciences, Analytical and Ecological Chemistry, Behringstraße, 54286 Trier, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2008-12</dateIssued><dateValid encoding="w3cdtf">2008-01-02</dateValid><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">3</extent><extent unit="tables">5</extent><extent unit="references">34</extent></physicalDescription><abstract lang="en">In spite of their low concentrations in soil solutions, low–molecular weight organic substances (LMWOS) such as amino acids, sugars, and uronic acids play a major role in the cycles of C and N in soil. With respect to their low concentrations and to possible matrix interferences, their analysis in soil leachates is a challenging task. We established two HPLC (high‐performance liquid chromatography) methods for the parallel determination of amino acids and carbohydrates in soil leachates. The pre‐column derivatization of amino acids with an o‐phthaldialdehyde (OPA) mercaptoethanol solution yields quantitation limits between 0.03 and 0.44 µmol L–1 and SD values of <8.3% (n = 9). High‐performance anion‐exchange chromatography (HPAEC) on a Dionex CarboPac PA 20 column with a NaOH acetate gradient combined with pulsed amperometric detection (PAD) was used for the determination of carbohydrates. The calibration curves obtained for 11 carbohydrates showed excellent linearity over the concentration range from 0.02 to 50.0 mg L–1. Recovery studies revealed good results for all analytes (89%–108%). Interferences from Hg(II) salts and chloroform used for stabilization of the leachates did not occur with both chromatographic methods. The optimized method was successfully used for quantitative determinations of amino acids and carbohydrates in soil leachates.</abstract><note type="funding">Deutsche Forschungsgemeinschaft</note><subject lang="en"><genre>keywords</genre><topic>low–molecular weight organic substances</topic><topic>amino acids</topic><topic>soil leachates</topic><topic>HPAEC‐PAD</topic><topic>carbohydrates</topic><topic>monosaccharides</topic><topic>amino sugars</topic><topic>uronic acids</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Plant Nutrition and Soil Science</title></titleInfo><titleInfo type="abbreviated"><title>Z. Pflanzenernähr. Bodenk.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Regular Article</topic></subject><identifier type="ISSN">1436-8730</identifier><identifier type="eISSN">1522-2624</identifier><identifier type="DOI">10.1002/(ISSN)1522-2624</identifier><identifier type="PublisherID">JPLN</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>171</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>917</start><end>926</end><total>10</total></extent></part></relatedItem><identifier type="istex">2F95600B2AA916EC9605741F49D343E9A5875B2A</identifier><identifier type="DOI">10.1002/jpln.200700235</identifier><identifier type="ArticleID">JPLN200700235</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. 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