Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene
Identifieur interne : 001413 ( Istex/Corpus ); précédent : 001412; suivant : 001414Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene
Auteurs : N. Tu ; H. Chen ; U. Winnikes ; I. Reinert ; K. M. Pirke ; K.-U. LentesSource :
- Life Sciences [ 0024-3205 ] ; 2000.
Abstract
Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5′-flanking region of the human UCP3 (hUCP3) gene. 5′-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cis-acting negative regulatory element between −2983 and −982 while the region between −982 and −284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.
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DOI: 10.1016/S0024-3205(00)00802-X
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Tu</name><affiliation><mods:affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</mods:affiliation></affiliation></author><author><name sortKey="Chen, H" sort="Chen, H" uniqKey="Chen H" first="H." last="Chen">H. Chen</name><affiliation><mods:affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</mods:affiliation></affiliation></author><author><name sortKey="Winnikes, U" sort="Winnikes, U" uniqKey="Winnikes U" first="U." last="Winnikes">U. Winnikes</name><affiliation><mods:affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</mods:affiliation></affiliation></author><author><name sortKey="Reinert, I" sort="Reinert, I" uniqKey="Reinert I" first="I." last="Reinert">I. Reinert</name><affiliation><mods:affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</mods:affiliation></affiliation></author><author><name sortKey="Pirke, K M" sort="Pirke, K M" uniqKey="Pirke K" first="K. M." last="Pirke">K. M. Pirke</name><affiliation><mods:affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</mods:affiliation></affiliation></author><author><name sortKey="Lentes, K U" sort="Lentes, K U" uniqKey="Lentes K" first="K.-U." last="Lentes">K.-U. Lentes</name><affiliation><mods:affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: kulentes@genprofile.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Life Sciences</title><title level="j" type="abbrev">LFS</title><idno type="ISSN">0024-3205</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">67</biblScope><biblScope unit="issue">18</biblScope><biblScope unit="page" from="2267">2267</biblScope><biblScope unit="page" to="2279">2279</biblScope></imprint><idno type="ISSN">0024-3205</idno></series><idno type="istex">188EEAC0770189267051BB7741508BB921669098</idno><idno type="DOI">10.1016/S0024-3205(00)00802-X</idno><idno type="PII">S0024-3205(00)00802-X</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0024-3205</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5′-flanking region of the human UCP3 (hUCP3) gene. 5′-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cis-acting negative regulatory element between −2983 and −982 while the region between −982 and −284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>N. Tu</name><affiliations><json:string>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</json:string></affiliations></json:item><json:item><name>H. Chen</name><affiliations><json:string>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</json:string></affiliations></json:item><json:item><name>U. Winnikes</name><affiliations><json:string>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</json:string></affiliations></json:item><json:item><name>I. Reinert</name><affiliations><json:string>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</json:string></affiliations></json:item><json:item><name>K.M. Pirke</name><affiliations><json:string>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</json:string></affiliations></json:item><json:item><name>K.-U. Lentes</name><affiliations><json:string>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</json:string><json:string>E-mail: kulentes@genprofile.com</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Human UCP3 promoter structure</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>UCP3 expression</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transcriptional regulation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Obesity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Energy expenditure</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Thermogenesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Body weight regulation</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Short communication</json:string></originalGenre><abstract>Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5′-flanking region of the human UCP3 (hUCP3) gene. 5′-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cis-acting negative regulatory element between −2983 and −982 while the region between −982 and −284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.</abstract><qualityIndicators><score>6.61</score><pdfVersion>1.2</pdfVersion><pdfPageSize>486 x 720 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>7</keywordCount><abstractCharCount>1023</abstractCharCount><pdfWordCount>4786</pdfWordCount><pdfCharCount>27449</pdfCharCount><pdfPageCount>13</pdfPageCount><abstractWordCount>152</abstractWordCount></qualityIndicators><title>Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene</title><pii><json:string>S0024-3205(00)00802-X</json:string></pii><refBibs><json:item><author><json:item><name>Bouillaud </name></json:item><json:item><name>M,F Seldin</name></json:item><json:item><name>R,S Surwit</name></json:item><json:item><name>D Ricquier</name></json:item><json:item><name>C,H Warden</name></json:item></author><host><pages><last>269</last><first>15</first></pages><author></author><title>Nat. 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Commun</title><publicationDate>1999</publicationDate></host><publicationDate>1999</publicationDate></json:item></refBibs><genre><json:string>brief-communication</json:string></genre><host><volume>67</volume><pii><json:string>S0024-3205(00)X0285-8</json:string></pii><pages><last>2279</last><first>2267</first></pages><issn><json:string>0024-3205</json:string></issn><issue>18</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Life Sciences</title><publicationDate>2000</publicationDate></host><categories><wos><json:string>science</json:string><json:string>pharmacology & pharmacy</json:string><json:string>medicine, research & experimental</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>pharmacology & pharmacy</json:string></scienceMetrix></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0024-3205(00)00802-X</json:string></doi><id>188EEAC0770189267051BB7741508BB921669098</id><score>1.3089755</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/188EEAC0770189267051BB7741508BB921669098/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/188EEAC0770189267051BB7741508BB921669098/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/188EEAC0770189267051BB7741508BB921669098/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2000 Elsevier Science Inc.</p></availability><date>2000</date></publicationStmt><notesStmt><note type="content">Section title: Original article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene</title><author xml:id="author-1"><persName><forename type="first">N.</forename><surname>Tu</surname></persName><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation></author><author xml:id="author-2"><persName><forename type="first">H.</forename><surname>Chen</surname></persName><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation></author><author xml:id="author-3"><persName><forename type="first">U.</forename><surname>Winnikes</surname></persName><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation></author><author xml:id="author-4"><persName><forename type="first">I.</forename><surname>Reinert</surname></persName><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation></author><author xml:id="author-5"><persName><forename type="first">K.M.</forename><surname>Pirke</surname></persName><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation></author><author xml:id="author-6"><persName><forename type="first">K.-U.</forename><surname>Lentes</surname></persName><email>kulentes@genprofile.com</email><note type="correspondence"><p>Corresponding author. GenProfile AG, Robert-Roessle-Strasse 10, D-13125 Berlin, Germany. Tel.: +49 30 9489-2149; fax: +49 30 9489-2166.(K.-U. Lentes)</p></note><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation></author></analytic><monogr><title level="j">Life Sciences</title><title level="j" type="abbrev">LFS</title><idno type="pISSN">0024-3205</idno><idno type="PII">S0024-3205(00)X0285-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">67</biblScope><biblScope unit="issue">18</biblScope><biblScope unit="page" from="2267">2267</biblScope><biblScope unit="page" to="2279">2279</biblScope></imprint></monogr><idno type="istex">188EEAC0770189267051BB7741508BB921669098</idno><idno type="DOI">10.1016/S0024-3205(00)00802-X</idno><idno type="PII">S0024-3205(00)00802-X</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5′-flanking region of the human UCP3 (hUCP3) gene. 5′-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cis-acting negative regulatory element between −2983 and −982 while the region between −982 and −284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Human UCP3 promoter structure</term></item><item><term>UCP3 expression</term></item><item><term>Transcriptional regulation</term></item><item><term>Obesity</term></item><item><term>Energy expenditure</term></item><item><term>Thermogenesis</term></item><item><term>Body weight regulation</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000-04-26">Modified</change><change when="2000">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-12-20">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-01-13">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-01-16">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/188EEAC0770189267051BB7741508BB921669098/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="sco"><item-info><jid>LFS</jid><aid>7802</aid><ce:pii>S0024-3205(00)00802-X</ce:pii><ce:doi>10.1016/S0024-3205(00)00802-X</ce:doi><ce:copyright type="full-transfer" year="2000">Elsevier Science Inc.</ce:copyright></item-info><head><ce:dochead><ce:textfn>Original article</ce:textfn></ce:dochead><ce:title>Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene</ce:title><ce:author-group><ce:author><ce:given-name>N.</ce:given-name><ce:surname>Tu</ce:surname></ce:author><ce:author><ce:given-name>H.</ce:given-name><ce:surname>Chen</ce:surname></ce:author><ce:author><ce:given-name>U.</ce:given-name><ce:surname>Winnikes</ce:surname></ce:author><ce:author><ce:given-name>I.</ce:given-name><ce:surname>Reinert</ce:surname></ce:author><ce:author><ce:given-name>K.M.</ce:given-name><ce:surname>Pirke</ce:surname></ce:author><ce:author><ce:given-name>K.-U.</ce:given-name><ce:surname>Lentes</ce:surname><ce:cross-ref refid="COR1">*</ce:cross-ref><ce:e-address>kulentes@genprofile.com</ce:e-address></ce:author><ce:affiliation><ce:textfn>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>*</ce:label><ce:text>Corresponding author. GenProfile AG, Robert-Roessle-Strasse 10, D-13125 Berlin, Germany. Tel.: +49 30 9489-2149; fax: +49 30 9489-2166.(K.-U. Lentes)</ce:text></ce:correspondence></ce:author-group><ce:date-received day="25" month="1" year="2000"></ce:date-received><ce:date-revised day="26" month="4" year="2000"></ce:date-revised><ce:date-accepted day="15" month="5" year="2000"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5′-flanking region of the human UCP3 (hUCP3) gene. 5′-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a <ce:italic>cis</ce:italic>-acting negative regulatory element between −2983 and −982 while the region between −982 and −284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C<ce:inf>2</ce:inf>C<ce:inf>12</ce:inf> reflecting the tissue-selective expression pattern of UCP3.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Human UCP3 promoter structure</ce:text></ce:keyword><ce:keyword><ce:text>UCP3 expression</ce:text></ce:keyword><ce:keyword><ce:text>Transcriptional regulation</ce:text></ce:keyword><ce:keyword><ce:text>Obesity</ce:text></ce:keyword><ce:keyword><ce:text>Energy expenditure</ce:text></ce:keyword><ce:keyword><ce:text>Thermogenesis</ce:text></ce:keyword><ce:keyword><ce:text>Body weight regulation</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Functional characterization of the 5′-flanking and the promoter region of the human UCP3 (hUCP3) gene</title></titleInfo><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Tu</namePart><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Chen</namePart><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">U.</namePart><namePart type="family">Winnikes</namePart><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Reinert</namePart><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.M.</namePart><namePart type="family">Pirke</namePart><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.-U.</namePart><namePart type="family">Lentes</namePart><affiliation>Laboratory of Molecular Neurogenetics, Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Friedrich-Wilhelm-Strasse 23, D-54290 Trier, Germany</affiliation><affiliation>E-mail: kulentes@genprofile.com</affiliation><description>Corresponding author. GenProfile AG, Robert-Roessle-Strasse 10, D-13125 Berlin, Germany. Tel.: +49 30 9489-2149; fax: +49 30 9489-2166.(K.-U. Lentes)</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><dateModified encoding="w3cdtf">2000-04-26</dateModified><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5′-flanking region of the human UCP3 (hUCP3) gene. 5′-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cis-acting negative regulatory element between −2983 and −982 while the region between −982 and −284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.</abstract><note type="content">Section title: Original article</note><subject><genre>Keywords</genre><topic>Human UCP3 promoter structure</topic><topic>UCP3 expression</topic><topic>Transcriptional regulation</topic><topic>Obesity</topic><topic>Energy expenditure</topic><topic>Thermogenesis</topic><topic>Body weight regulation</topic></subject><relatedItem type="host"><titleInfo><title>Life Sciences</title></titleInfo><titleInfo type="abbreviated"><title>LFS</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">20000922</dateIssued></originInfo><identifier type="ISSN">0024-3205</identifier><identifier type="PII">S0024-3205(00)X0285-8</identifier><part><date>20000922</date><detail type="volume"><number>67</number><caption>vol.</caption></detail><detail type="issue"><number>18</number><caption>no.</caption></detail><extent unit="issue pages"><start>2147</start><end>2280</end></extent><extent unit="pages"><start>2267</start><end>2279</end></extent></part></relatedItem><identifier type="istex">188EEAC0770189267051BB7741508BB921669098</identifier><identifier type="DOI">10.1016/S0024-3205(00)00802-X</identifier><identifier type="PII">S0024-3205(00)00802-X</identifier><accessCondition type="use and reproduction" contentType="copyright">©2000 Elsevier Science Inc.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Inc., ©2000</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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