Cryopreservation of early stage Siberian sturgeon Acipenser baerii germ cells, comparison of whole tissue and dissociated cells.
Identifieur interne : 000073 ( PubMed/Corpus ); précédent : 000072; suivant : 000074Cryopreservation of early stage Siberian sturgeon Acipenser baerii germ cells, comparison of whole tissue and dissociated cells.
Auteurs : Martin Pšeni Ka ; Taiju Saito ; Marek Rodina ; Boris DzyubaSource :
- Cryobiology [ 1090-2392 ] ; 2016.
English descriptors
- KwdEn :
- Animals, Cell Proliferation (drug effects), Cell Survival (drug effects), Cryopreservation (methods), Cryoprotective Agents (pharmacology), Dimethyl Sulfoxide (pharmacology), Endangered Species, Ethylene Glycol (pharmacology), Female, Fishes (physiology), Freezing, Glycerol (pharmacology), Male, Organ Culture Techniques, Ovum (cytology), Ovum (drug effects), Ovum (transplantation), Propylene Glycol (pharmacology), Spermatozoa (cytology), Spermatozoa (drug effects), Spermatozoa (transplantation).
- MESH :
- chemical , pharmacology : Cryoprotective Agents, Dimethyl Sulfoxide, Ethylene Glycol, Glycerol, Propylene Glycol.
- cytology : Ovum, Spermatozoa.
- drug effects : Cell Proliferation, Cell Survival, Ovum, Spermatozoa.
- methods : Cryopreservation.
- physiology : Fishes.
- transplantation : Ovum, Spermatozoa.
- Animals, Endangered Species, Female, Freezing, Male, Organ Culture Techniques.
Abstract
Several sturgeon species are near extinction; therefore an efficient conservation strategy is required. Germ stem cells can be used for long-term storage and restoration of genetic information using surrogate reproduction. This study compared cryopreservation procedures of early stages of Siberian sturgeon Acipenser baerii testicular and ovarian cells. Whole gonad tissue or dissociated cells were frozen at a cooling rate of 1 °C/min in phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and one of four different 1.5 M cryoprotectants: dimethyl sulfoxide, glycerol, ethylene glycol, or dimethyl sulfoxide with propanediol. The number of living cells obtained from 0.1 g of gonadal tissue after freeze/thaw of both whole tissue and dissociated cells was higher using ethylene glycol than with other cryoprotectants. Although there were no differences in the number of living cells in cryopreserved whole tissue vs. dissociated cells, the number of dead cells was lower with whole tissue cryopreservation, indicating that cells that died during freeze/thaw were digested during subsequent enzymatic dissociation. This resulted in more than 90% live cells after freeze/thaw and dissociation. The thawed tissue cryopreserved using ethylene glycol as protectant as well as fresh gonadal tissue were dissociated, and the cells were labelled by PKH26 and transplanted into larvae of sterlet Acipenser ruthenus. Ninety days post-transplant of both fresh and cryopreserved cells, introduced cells proliferated in more than half of the recipients.
DOI: 10.1016/j.cryobiol.2016.02.005
PubMed: 26920821
Links to Exploration step
pubmed:26920821Le document en format XML
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Electronic address: psenicka@frov.jcu.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Saito, Taiju" sort="Saito, Taiju" uniqKey="Saito T" first="Taiju" last="Saito">Taiju Saito</name><affiliation><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name><affiliation><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Dzyuba, Boris" sort="Dzyuba, Boris" uniqKey="Dzyuba B" first="Boris" last="Dzyuba">Boris Dzyuba</name><affiliation><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26920821</idno><idno type="pmid">26920821</idno><idno type="doi">10.1016/j.cryobiol.2016.02.005</idno><idno type="wicri:Area/PubMed/Corpus">000073</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000073</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cryopreservation of early stage Siberian sturgeon Acipenser baerii germ cells, comparison of whole tissue and dissociated cells.</title><author><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name><affiliation><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: psenicka@frov.jcu.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Saito, Taiju" sort="Saito, Taiju" uniqKey="Saito T" first="Taiju" last="Saito">Taiju Saito</name><affiliation><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name><affiliation><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Dzyuba, Boris" sort="Dzyuba, Boris" uniqKey="Dzyuba B" first="Boris" last="Dzyuba">Boris Dzyuba</name><affiliation><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Cryobiology</title><idno type="eISSN">1090-2392</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Proliferation (drug effects)</term><term>Cell Survival (drug effects)</term><term>Cryopreservation (methods)</term><term>Cryoprotective Agents (pharmacology)</term><term>Dimethyl Sulfoxide (pharmacology)</term><term>Endangered Species</term><term>Ethylene Glycol (pharmacology)</term><term>Female</term><term>Fishes (physiology)</term><term>Freezing</term><term>Glycerol (pharmacology)</term><term>Male</term><term>Organ Culture Techniques</term><term>Ovum (cytology)</term><term>Ovum (drug effects)</term><term>Ovum (transplantation)</term><term>Propylene Glycol (pharmacology)</term><term>Spermatozoa (cytology)</term><term>Spermatozoa (drug effects)</term><term>Spermatozoa (transplantation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cryoprotective Agents</term><term>Dimethyl Sulfoxide</term><term>Ethylene Glycol</term><term>Glycerol</term><term>Propylene Glycol</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Ovum</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term><term>Cell Survival</term><term>Ovum</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cryopreservation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="transplantation" xml:lang="en"><term>Ovum</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Endangered Species</term><term>Female</term><term>Freezing</term><term>Male</term><term>Organ Culture Techniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Several sturgeon species are near extinction; therefore an efficient conservation strategy is required. Germ stem cells can be used for long-term storage and restoration of genetic information using surrogate reproduction. This study compared cryopreservation procedures of early stages of Siberian sturgeon Acipenser baerii testicular and ovarian cells. Whole gonad tissue or dissociated cells were frozen at a cooling rate of 1 °C/min in phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and one of four different 1.5 M cryoprotectants: dimethyl sulfoxide, glycerol, ethylene glycol, or dimethyl sulfoxide with propanediol. The number of living cells obtained from 0.1 g of gonadal tissue after freeze/thaw of both whole tissue and dissociated cells was higher using ethylene glycol than with other cryoprotectants. Although there were no differences in the number of living cells in cryopreserved whole tissue vs. dissociated cells, the number of dead cells was lower with whole tissue cryopreservation, indicating that cells that died during freeze/thaw were digested during subsequent enzymatic dissociation. This resulted in more than 90% live cells after freeze/thaw and dissociation. The thawed tissue cryopreserved using ethylene glycol as protectant as well as fresh gonadal tissue were dissociated, and the cells were labelled by PKH26 and transplanted into larvae of sterlet Acipenser ruthenus. Ninety days post-transplant of both fresh and cryopreserved cells, introduced cells proliferated in more than half of the recipients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26920821</PMID><DateCreated><Year>2016</Year><Month>04</Month><Day>06</Day></DateCreated><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2392</ISSN><JournalIssue CitedMedium="Internet"><Volume>72</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Cryobiology</Title><ISOAbbreviation>Cryobiology</ISOAbbreviation></Journal><ArticleTitle>Cryopreservation of early stage Siberian sturgeon Acipenser baerii germ cells, comparison of whole tissue and dissociated cells.</ArticleTitle><Pagination><MedlinePgn>119-22</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cryobiol.2016.02.005</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0011-2240(16)30008-6</ELocationID><Abstract><AbstractText>Several sturgeon species are near extinction; therefore an efficient conservation strategy is required. Germ stem cells can be used for long-term storage and restoration of genetic information using surrogate reproduction. This study compared cryopreservation procedures of early stages of Siberian sturgeon Acipenser baerii testicular and ovarian cells. Whole gonad tissue or dissociated cells were frozen at a cooling rate of 1 °C/min in phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and one of four different 1.5 M cryoprotectants: dimethyl sulfoxide, glycerol, ethylene glycol, or dimethyl sulfoxide with propanediol. The number of living cells obtained from 0.1 g of gonadal tissue after freeze/thaw of both whole tissue and dissociated cells was higher using ethylene glycol than with other cryoprotectants. Although there were no differences in the number of living cells in cryopreserved whole tissue vs. dissociated cells, the number of dead cells was lower with whole tissue cryopreservation, indicating that cells that died during freeze/thaw were digested during subsequent enzymatic dissociation. This resulted in more than 90% live cells after freeze/thaw and dissociation. The thawed tissue cryopreserved using ethylene glycol as protectant as well as fresh gonadal tissue were dissociated, and the cells were labelled by PKH26 and transplanted into larvae of sterlet Acipenser ruthenus. Ninety days post-transplant of both fresh and cryopreserved cells, introduced cells proliferated in more than half of the recipients.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pšenička</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: psenicka@frov.jcu.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>Taiju</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodina</LastName><ForeName>Marek</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dzyuba</LastName><ForeName>Boris</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Cryobiology</MedlineTA><NlmUniqueID>0006252</NlmUniqueID><ISSNLinking>0011-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003451">Cryoprotective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>6DC9Q167V3</RegistryNumber><NameOfSubstance UI="D019946">Propylene Glycol</NameOfSubstance></Chemical><Chemical><RegistryNumber>FC72KVT52F</RegistryNumber><NameOfSubstance UI="D019855">Ethylene Glycol</NameOfSubstance></Chemical><Chemical><RegistryNumber>PDC6A3C0OX</RegistryNumber><NameOfSubstance UI="D005990">Glycerol</NameOfSubstance></Chemical><Chemical><RegistryNumber>YOW8V9698H</RegistryNumber><NameOfSubstance UI="D004121">Dimethyl Sulfoxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015925" MajorTopicYN="N">Cryopreservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003451" MajorTopicYN="N">Cryoprotective Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004121" MajorTopicYN="N">Dimethyl Sulfoxide</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056727" MajorTopicYN="N">Endangered Species</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019855" MajorTopicYN="N">Ethylene Glycol</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005615" MajorTopicYN="N">Freezing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005990" MajorTopicYN="N">Glycerol</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009924" MajorTopicYN="N">Organ Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010063" MajorTopicYN="N">Ovum</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000637" MajorTopicYN="N">transplantation</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019946" MajorTopicYN="N">Propylene Glycol</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000637" MajorTopicYN="N">transplantation</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cryopreservation</Keyword><Keyword MajorTopicYN="N">Germ cells</Keyword><Keyword MajorTopicYN="N">Germ-line chimera</Keyword><Keyword MajorTopicYN="N">Oogonia</Keyword><Keyword MajorTopicYN="N">Spermatogonia</Keyword><Keyword MajorTopicYN="N">Sturgeon</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>08</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>02</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>02</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26920821</ArticleId><ArticleId IdType="pii">S0011-2240(16)30008-6</ArticleId><ArticleId IdType="doi">10.1016/j.cryobiol.2016.02.005</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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