Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.
Identifieur interne : 000072 ( PubMed/Corpus ); précédent : 000071; suivant : 000073Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.
Auteurs : Amin Golpour ; Mohammad Abdul Momin Siddique ; Marek Rodina ; Martin Pšeni KaSource :
- Cryobiology [ 1090-2392 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Cryoprotective Agents, Ethylene Glycol.
- cytology : Testis.
- methods : Cryopreservation, Organ Preservation.
- Animals, Cell Survival, Endangered Species, Fishes, Male, Temperature.
Abstract
The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 °C at a cooling rate of 1 °C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 °C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 °C or in LN. This result indicated that testicular cells can be stored at -80 °C and/or on dry ice, for 2 days with minimum loss of viability.
DOI: 10.1016/j.cryobiol.2016.03.002
PubMed: 26964775
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Electronic address: dehsari@frov.jcu.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Siddique, Mohammad Abdul Momin" sort="Siddique, Mohammad Abdul Momin" uniqKey="Siddique M" first="Mohammad Abdul Momin" last="Siddique">Mohammad Abdul Momin Siddique</name><affiliation><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name><affiliation><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name><affiliation><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26964775</idno><idno type="pmid">26964775</idno><idno type="doi">10.1016/j.cryobiol.2016.03.002</idno><idno type="wicri:Area/PubMed/Corpus">000072</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000072</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.</title><author><name sortKey="Golpour, Amin" sort="Golpour, Amin" uniqKey="Golpour A" first="Amin" last="Golpour">Amin Golpour</name><affiliation><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: dehsari@frov.jcu.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Siddique, Mohammad Abdul Momin" sort="Siddique, Mohammad Abdul Momin" uniqKey="Siddique M" first="Mohammad Abdul Momin" last="Siddique">Mohammad Abdul Momin Siddique</name><affiliation><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name><affiliation><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name><affiliation><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Cryobiology</title><idno type="eISSN">1090-2392</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Survival</term><term>Cryopreservation (methods)</term><term>Cryoprotective Agents (pharmacology)</term><term>Endangered Species</term><term>Ethylene Glycol (pharmacology)</term><term>Fishes</term><term>Male</term><term>Organ Preservation (methods)</term><term>Temperature</term><term>Testis (cytology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cryoprotective Agents</term><term>Ethylene Glycol</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Testis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cryopreservation</term><term>Organ Preservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Survival</term><term>Endangered Species</term><term>Fishes</term><term>Male</term><term>Temperature</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 °C at a cooling rate of 1 °C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 °C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 °C or in LN. This result indicated that testicular cells can be stored at -80 °C and/or on dry ice, for 2 days with minimum loss of viability.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26964775</PMID><DateCreated><Year>2016</Year><Month>04</Month><Day>06</Day></DateCreated><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2392</ISSN><JournalIssue CitedMedium="Internet"><Volume>72</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Cryobiology</Title><ISOAbbreviation>Cryobiology</ISOAbbreviation></Journal><ArticleTitle>Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.</ArticleTitle><Pagination><MedlinePgn>154-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cryobiol.2016.03.002</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0011-2240(16)30013-X</ELocationID><Abstract><AbstractText>The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 °C at a cooling rate of 1 °C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 °C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 °C or in LN. This result indicated that testicular cells can be stored at -80 °C and/or on dry ice, for 2 days with minimum loss of viability.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Golpour</LastName><ForeName>Amin</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: dehsari@frov.jcu.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Siddique</LastName><ForeName>Mohammad Abdul Momin</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodina</LastName><ForeName>Marek</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pšenička</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Cryobiology</MedlineTA><NlmUniqueID>0006252</NlmUniqueID><ISSNLinking>0011-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003451">Cryoprotective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>FC72KVT52F</RegistryNumber><NameOfSubstance UI="D019855">Ethylene Glycol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015925" MajorTopicYN="N">Cryopreservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003451" MajorTopicYN="N">Cryoprotective Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056727" MajorTopicYN="N">Endangered Species</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019855" MajorTopicYN="N">Ethylene Glycol</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="Y">Fishes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009926" MajorTopicYN="N">Organ Preservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" 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