Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa.
Identifieur interne : 000245 ( PubMed/Checkpoint ); précédent : 000244; suivant : 000246Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa.
Auteurs : Ievgeniia Gazo [République tchèque] ; Pavla Linhartova ; Anna Shaliutina ; Martin HulakSource :
- Chemico-biological interactions [ 1872-7786 ] ; 2013.
English descriptors
- KwdEn :
- Adenosine Triphosphate (metabolism), Animals, Antioxidants (metabolism), DNA (genetics), DNA Fragmentation (drug effects), Dose-Response Relationship, Drug, Environment, Fishes (genetics), Fishes (metabolism), Fungicides, Industrial (adverse effects), Male, Oxazoles (adverse effects), Oxidative Stress (drug effects), Sperm Motility (drug effects), Spermatozoa (cytology), Spermatozoa (drug effects), Spermatozoa (metabolism), Water Pollutants, Chemical (adverse effects).
- MESH :
- chemical , adverse effects : Fungicides, Industrial, Oxazoles, Water Pollutants, Chemical.
- chemical , genetics : DNA.
- chemical , metabolism : Adenosine Triphosphate, Antioxidants.
- cytology : Spermatozoa.
- drug effects : DNA Fragmentation, Oxidative Stress, Sperm Motility, Spermatozoa.
- genetics : Fishes.
- metabolism : Fishes, Spermatozoa.
- Animals, Dose-Response Relationship, Drug, Environment, Male.
Abstract
The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 μg/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 μg/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 μg/l and above. After 2 h exposure at higher test concentrations (10-50 μg/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system.
DOI: 10.1016/j.cbi.2013.01.004
PubMed: 23376258
Affiliations:
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and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia, Zatisi 728/11, Vodnany 32901</wicri:regionArea><wicri:noRegion>Vodnany 32901</wicri:noRegion></affiliation></author><author><name sortKey="Linhartova, Pavla" sort="Linhartova, Pavla" uniqKey="Linhartova P" first="Pavla" last="Linhartova">Pavla Linhartova</name></author><author><name sortKey="Shaliutina, Anna" sort="Shaliutina, Anna" uniqKey="Shaliutina A" first="Anna" last="Shaliutina">Anna Shaliutina</name></author><author><name sortKey="Hulak, Martin" sort="Hulak, Martin" uniqKey="Hulak M" first="Martin" last="Hulak">Martin Hulak</name></author></analytic><series><title level="j">Chemico-biological interactions</title><idno type="eISSN">1872-7786</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate (metabolism)</term><term>Animals</term><term>Antioxidants (metabolism)</term><term>DNA (genetics)</term><term>DNA Fragmentation (drug effects)</term><term>Dose-Response Relationship, Drug</term><term>Environment</term><term>Fishes (genetics)</term><term>Fishes (metabolism)</term><term>Fungicides, Industrial (adverse effects)</term><term>Male</term><term>Oxazoles (adverse effects)</term><term>Oxidative Stress (drug effects)</term><term>Sperm Motility (drug effects)</term><term>Spermatozoa (cytology)</term><term>Spermatozoa (drug effects)</term><term>Spermatozoa (metabolism)</term><term>Water Pollutants, Chemical (adverse effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Fungicides, Industrial</term><term>Oxazoles</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine Triphosphate</term><term>Antioxidants</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>DNA Fragmentation</term><term>Oxidative Stress</term><term>Sperm Motility</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Dose-Response Relationship, Drug</term><term>Environment</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 μg/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 μg/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 μg/l and above. After 2 h exposure at higher test concentrations (10-50 μg/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23376258</PMID><DateCreated><Year>2013</Year><Month>04</Month><Day>29</Day></DateCreated><DateCompleted><Year>2013</Year><Month>06</Month><Day>26</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7786</ISSN><JournalIssue CitedMedium="Internet"><Volume>203</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Apr</Month><Day>25</Day></PubDate></JournalIssue><Title>Chemico-biological interactions</Title><ISOAbbreviation>Chem. Biol. Interact.</ISOAbbreviation></Journal><ArticleTitle>Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa.</ArticleTitle><Pagination><MedlinePgn>377-85</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cbi.2013.01.004</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0009-2797(13)00007-0</ELocationID><Abstract><AbstractText>The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 μg/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 μg/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 μg/l and above. After 2 h exposure at higher test concentrations (10-50 μg/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gazo</LastName><ForeName>Ievgeniia</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia, Zatisi 728/11, Vodnany 32901, Czech Republic. gazo@frov.jcu.cz</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Linhartova</LastName><ForeName>Pavla</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Shaliutina</LastName><ForeName>Anna</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Hulak</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research 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effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>12</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>01</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>2</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>2</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23376258</ArticleId><ArticleId IdType="pii">S0009-2797(13)00007-0</ArticleId><ArticleId IdType="doi">10.1016/j.cbi.2013.01.004</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République tchèque</li></country></list><tree><noCountry><name sortKey="Hulak, Martin" sort="Hulak, Martin" uniqKey="Hulak M" first="Martin" last="Hulak">Martin Hulak</name><name sortKey="Linhartova, Pavla" sort="Linhartova, Pavla" uniqKey="Linhartova P" first="Pavla" last="Linhartova">Pavla Linhartova</name><name sortKey="Shaliutina, Anna" sort="Shaliutina, Anna" uniqKey="Shaliutina A" first="Anna" last="Shaliutina">Anna Shaliutina</name></noCountry><country name="République tchèque"><noRegion><name sortKey="Gazo, Ievgeniia" sort="Gazo, Ievgeniia" uniqKey="Gazo I" first="Ievgeniia" last="Gazo">Ievgeniia Gazo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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