Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa.
Identifieur interne : 000260 ( PubMed/Corpus ); précédent : 000259; suivant : 000261Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa.
Auteurs : Ievgeniia Gazo ; Pavla Linhartova ; Anna Shaliutina ; Martin HulakSource :
- Chemico-biological interactions [ 1872-7786 ] ; 2013.
English descriptors
- KwdEn :
- Adenosine Triphosphate (metabolism), Animals, Antioxidants (metabolism), DNA (genetics), DNA Fragmentation (drug effects), Dose-Response Relationship, Drug, Environment, Fishes (genetics), Fishes (metabolism), Fungicides, Industrial (adverse effects), Male, Oxazoles (adverse effects), Oxidative Stress (drug effects), Sperm Motility (drug effects), Spermatozoa (cytology), Spermatozoa (drug effects), Spermatozoa (metabolism), Water Pollutants, Chemical (adverse effects).
- MESH :
- chemical , adverse effects : Fungicides, Industrial, Oxazoles, Water Pollutants, Chemical.
- chemical , genetics : DNA.
- chemical , metabolism : Adenosine Triphosphate, Antioxidants.
- cytology : Spermatozoa.
- drug effects : DNA Fragmentation, Oxidative Stress, Sperm Motility, Spermatozoa.
- genetics : Fishes.
- metabolism : Fishes, Spermatozoa.
- Animals, Dose-Response Relationship, Drug, Environment, Male.
Abstract
The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 μg/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 μg/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 μg/l and above. After 2 h exposure at higher test concentrations (10-50 μg/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system.
DOI: 10.1016/j.cbi.2013.01.004
PubMed: 23376258
Links to Exploration step
pubmed:23376258Le document en format XML
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<author><name sortKey="Linhartova, Pavla" sort="Linhartova, Pavla" uniqKey="Linhartova P" first="Pavla" last="Linhartova">Pavla Linhartova</name>
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<author><name sortKey="Shaliutina, Anna" sort="Shaliutina, Anna" uniqKey="Shaliutina A" first="Anna" last="Shaliutina">Anna Shaliutina</name>
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<front><div type="abstract" xml:lang="en">The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 μg/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 μg/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 μg/l and above. After 2 h exposure at higher test concentrations (10-50 μg/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system.</div>
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<Abstract><AbstractText>The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 μg/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 μg/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 μg/l and above. After 2 h exposure at higher test concentrations (10-50 μg/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system.</AbstractText>
<CopyrightInformation>Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.</CopyrightInformation>
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