Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay
Identifieur interne : 000160 ( PascalFrancis/Curation ); précédent : 000159; suivant : 000161Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay
Auteurs : Kevin T. Kwak [États-Unis] ; Ian A. Gardner [États-Unis] ; Thomas B. Farver [États-Unis] ; Ronald P. Hedrick [États-Unis]Source :
- Aquaculture : (Amsterdam) [ 0044-8486 ] ; 2006.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Histologie, Aquiculture.
English descriptors
- KwdEn :
Abstract
A polymerase chain reaction (PCR) assay was developed for detection of white sturgeon iridovirus (WSIV). The synthetic oligonucleotides WS 229F and WS 245R generate a 519 bp DNA amplicon from the WSIV genome that encodes the putative major capsid protein (MCP) gene. The WSIV PCR detected as little as 1 fg of plasmid DNA as mixed with 100 ng of host DNA. No amplicons were detected with the WSIV PCR assay upon testing of genomic DNA from uninfected white sturgeon spleen cell line (WSS-2), uninfected white sturgeon tissues, white sturgeon adenovirus (WSAV), Acipenserid herpesvirus 2 (AcHV-2), frog virus 3 (FV-3), epizootic haematopoietic necrosis virus (EHNV), and red sea bream iridovirus (RSIV). The newly developed PCR assay was also evaluated with groups of juvenile white sturgeon following experimental exposures to WSIV. An analysis of WSIV detection by PCR compared to current histological methods provided sensitivity estimates of 98% and 64% for PCR and histology, respectively. This new PCR assay provides a more rapid and sensitive approach for the detection of WSIV infections among juvenile white sturgeon than currently available diagnostic approaches including virus isolation and microscopic examinations of stained tissue sections.
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CA 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Farver, Thomas B" sort="Farver, Thomas B" uniqKey="Farver T" first="Thomas B." last="Farver">Thomas B. Farver</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Population Health and Reproduction, School of Veterinary Medicine, University of California</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Hedrick, Ronald P" sort="Hedrick, Ronald P" uniqKey="Hedrick R" first="Ronald P." last="Hedrick">Ronald P. Hedrick</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1><s2>Davis. 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CA 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Gardner, Ian A" sort="Gardner, Ian A" uniqKey="Gardner I" first="Ian A." last="Gardner">Ian A. Gardner</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1><s2>Davis. CA 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Farver, Thomas B" sort="Farver, Thomas B" uniqKey="Farver T" first="Thomas B." last="Farver">Thomas B. Farver</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Population Health and Reproduction, School of Veterinary Medicine, University of California</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author><author><name sortKey="Hedrick, Ronald P" sort="Hedrick, Ronald P" uniqKey="Hedrick R" first="Ronald P." last="Hedrick">Ronald P. Hedrick</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1><s2>Davis. CA 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country></affiliation></author></analytic><series><title level="j" type="main">Aquaculture : (Amsterdam)</title><title level="j" type="abbreviated">Aquaculture : (Amst.)</title><idno type="ISSN">0044-8486</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Aquaculture : (Amsterdam)</title><title level="j" type="abbreviated">Aquaculture : (Amst.)</title><idno type="ISSN">0044-8486</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term><term>Aquaculture</term><term>Capsid</term><term>Detection</term><term>Histology</term><term>Iridovirus</term><term>Polymerase chain reaction</term><term>Protein</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Détection</term><term>Réaction chaîne polymérase</term><term>Histologie</term><term>Capside</term><term>Protéine</term><term>Aquiculture</term><term>Acipenser transmontanus</term><term>Iridovirus</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Histologie</term><term>Aquiculture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A polymerase chain reaction (PCR) assay was developed for detection of white sturgeon iridovirus (WSIV). The synthetic oligonucleotides WS 229F and WS 245R generate a 519 bp DNA amplicon from the WSIV genome that encodes the putative major capsid protein (MCP) gene. The WSIV PCR detected as little as 1 fg of plasmid DNA as mixed with 100 ng of host DNA. No amplicons were detected with the WSIV PCR assay upon testing of genomic DNA from uninfected white sturgeon spleen cell line (WSS-2), uninfected white sturgeon tissues, white sturgeon adenovirus (WSAV), Acipenserid herpesvirus 2 (AcHV-2), frog virus 3 (FV-3), epizootic haematopoietic necrosis virus (EHNV), and red sea bream iridovirus (RSIV). The newly developed PCR assay was also evaluated with groups of juvenile white sturgeon following experimental exposures to WSIV. An analysis of WSIV detection by PCR compared to current histological methods provided sensitivity estimates of 98% and 64% for PCR and histology, respectively. This new PCR assay provides a more rapid and sensitive approach for the detection of WSIV infections among juvenile white sturgeon than currently available diagnostic approaches including virus isolation and microscopic examinations of stained tissue sections.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0044-8486</s0></fA01><fA02 i1="01"><s0>AQCLAL</s0></fA02><fA03 i2="1"><s0>Aquaculture : (Amst.)</s0></fA03><fA05><s2>254</s2></fA05><fA06><s2>1-4</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay</s1></fA08><fA11 i1="01" i2="1"><s1>KWAK (Kevin T.)</s1></fA11><fA11 i1="02" i2="1"><s1>GARDNER (Ian A.)</s1></fA11><fA11 i1="03" i2="1"><s1>FARVER (Thomas B.)</s1></fA11><fA11 i1="04" i2="1"><s1>HEDRICK (Ronald P.)</s1></fA11><fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1><s2>Davis. CA 95616</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Population Health and Reproduction, School of Veterinary Medicine, University of California</s1><s2>Davis, CA 95616</s2><s3>USA</s3><sZ>3 aut.</sZ></fA14><fA20><s1>92-101</s1></fA20><fA21><s1>2006</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>15964</s2><s5>354000142636520120</s5></fA43><fA44><s0>0000</s0><s1>© 2006 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>28 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>06-0265987</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Aquaculture : (Amsterdam)</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>A polymerase chain reaction (PCR) assay was developed for detection of white sturgeon iridovirus (WSIV). 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