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Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii

Identifieur interne : 000100 ( PascalFrancis/Corpus ); précédent : 000099; suivant : 000101

Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii

Auteurs : Zhi-Hua Li ; PING LI ; Marek Rodina ; Tomas Randak

Source :

RBID : Pascal:10-0510437

Descripteurs français

English descriptors

Abstract

In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2 h at 50 μg/L VRP, 5.0 μg/L Cd, the mixture of 50 μg/L VRP + 5.0 μg/L Cd, 50 μg/L Cd and the mixture of 50 μg/L VRP + 50 μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0 μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0166-445X
A02 01      @0 AQTODG
A03   1    @0 Aquat. toxicol.
A05       @2 100
A06       @2 4
A08 01  1  ENG  @1 Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii
A11 01  1    @1 LI (Zhi-Hua)
A11 02  1    @1 PING LI
A11 03  1    @1 RODINA (Marek)
A11 04  1    @1 RANDAK (Tomas)
A14 01      @1 University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II @2 389 25 Vodnany @3 CZE @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.
A14 02      @1 Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences @2 Jingzhou 434000 @3 CHN @Z 1 aut. @Z 2 aut.
A20       @1 373-375
A21       @1 2010
A23 01      @0 ENG
A43 01      @1 INIST @2 18841 @5 354000192532030090
A44       @0 0000 @1 © 2010 INIST-CNRS. All rights reserved.
A45       @0 1/4 p.
A47 01  1    @0 10-0510437
A60       @1 P
A61       @0 A
A64 01  1    @0 Aquatic toxicology
A66 01      @0 NLD
C01 01    ENG  @0 In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2 h at 50 μg/L VRP, 5.0 μg/L Cd, the mixture of 50 μg/L VRP + 5.0 μg/L Cd, 50 μg/L Cd and the mixture of 50 μg/L VRP + 50 μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0 μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.
C02 01  X    @0 002A14D05A
C02 02  X    @0 002A15B
C03 01  X  FRE  @0 Calcium @2 NC @2 FR @5 01
C03 01  X  ENG  @0 Calcium @2 NC @2 FR @5 01
C03 01  X  SPA  @0 Calcio @2 NC @2 FR @5 01
C03 02  X  FRE  @0 Cadmium @2 NC @2 FX @5 02
C03 02  X  ENG  @0 Cadmium @2 NC @2 FX @5 02
C03 02  X  SPA  @0 Cadmio @2 NC @2 FX @5 02
C03 03  X  FRE  @0 Stress @5 03
C03 03  X  ENG  @0 Stress @5 03
C03 03  X  SPA  @0 Estrés @5 03
C03 04  X  FRE  @0 Spermatozoïde @5 04
C03 04  X  ENG  @0 Spermatozoa @5 04
C03 04  X  SPA  @0 Espermatozoide @5 04
C03 05  X  FRE  @0 Russe @5 05
C03 05  X  ENG  @0 Russian @5 05
C03 05  X  SPA  @0 Ruso @5 05
C03 06  X  FRE  @0 Motilité @5 06
C03 06  X  ENG  @0 Motility @5 06
C03 06  X  SPA  @0 Motilidad @5 06
C03 07  X  FRE  @0 Antioxydant @5 07
C03 07  X  ENG  @0 Antioxidant @5 07
C03 07  X  SPA  @0 Antioxidante @5 07
C03 08  X  FRE  @0 Milieu aquatique @5 08
C03 08  X  ENG  @0 Aquatic environment @5 08
C03 08  X  SPA  @0 Medio acuático @5 08
C03 09  X  FRE  @0 Ecotoxicologie @5 09
C03 09  X  ENG  @0 Ecotoxicology @5 09
C03 09  X  SPA  @0 Ecotoxicología @5 09
C03 10  X  FRE  @0 Toxicité @5 23
C03 10  X  ENG  @0 Toxicity @5 23
C03 10  X  SPA  @0 Toxicidad @5 23
C03 11  X  FRE  @0 Polluant @5 24
C03 11  X  ENG  @0 Pollutant @5 24
C03 11  X  SPA  @0 Contaminante @5 24
C03 12  X  FRE  @0 Vérapamil @2 NK @2 FR @5 41
C03 12  X  ENG  @0 Verapamil @2 NK @2 FR @5 41
C03 12  X  SPA  @0 Verapamilo @2 NK @2 FR @5 41
C03 13  X  FRE  @0 Acipenser @4 INC @5 64
C07 01  X  FRE  @0 Métal lourd @5 17
C07 01  X  ENG  @0 Heavy metal @5 17
C07 01  X  SPA  @0 Metal pesado @5 17
C07 02  X  FRE  @0 Pisces @2 NS @5 29
C07 02  X  ENG  @0 Pisces @2 NS @5 29
C07 02  X  SPA  @0 Pisces @2 NS @5 29
C07 03  X  FRE  @0 Vertebrata @2 NS
C07 03  X  ENG  @0 Vertebrata @2 NS
C07 03  X  SPA  @0 Vertebrata @2 NS
C07 04  X  FRE  @0 Acipenseridae @4 INC @5 70
N21       @1 341
N44 01      @1 OTO
N82       @1 OTO

Format Inist (serveur)

NO : PASCAL 10-0510437 INIST
ET : Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii
AU : LI (Zhi-Hua); PING LI; RODINA (Marek); RANDAK (Tomas)
AF : University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II/389 25 Vodnany/Tchèque, République (1 aut., 2 aut., 3 aut., 4 aut.); Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences/Jingzhou 434000/Chine (1 aut., 2 aut.)
DT : Publication en série; Niveau analytique
SO : Aquatic toxicology; ISSN 0166-445X; Coden AQTODG; Pays-Bas; Da. 2010; Vol. 100; No. 4; Pp. 373-375; Bibl. 1/4 p.
LA : Anglais
EA : In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2 h at 50 μg/L VRP, 5.0 μg/L Cd, the mixture of 50 μg/L VRP + 5.0 μg/L Cd, 50 μg/L Cd and the mixture of 50 μg/L VRP + 50 μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0 μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.
CC : 002A14D05A; 002A15B
FD : Calcium; Cadmium; Stress; Spermatozoïde; Russe; Motilité; Antioxydant; Milieu aquatique; Ecotoxicologie; Toxicité; Polluant; Vérapamil; Acipenser
FG : Métal lourd; Pisces; Vertebrata; Acipenseridae
ED : Calcium; Cadmium; Stress; Spermatozoa; Russian; Motility; Antioxidant; Aquatic environment; Ecotoxicology; Toxicity; Pollutant; Verapamil
EG : Heavy metal; Pisces; Vertebrata
SD : Calcio; Cadmio; Estrés; Espermatozoide; Ruso; Motilidad; Antioxidante; Medio acuático; Ecotoxicología; Toxicidad; Contaminante; Verapamilo
LO : INIST-18841.354000192532030090
ID : 10-0510437

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Pascal:10-0510437

Le document en format XML

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<div type="abstract" xml:lang="en">In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2 h at 50 μg/L VRP, 5.0 μg/L Cd, the mixture of 50 μg/L VRP + 5.0 μg/L Cd, 50 μg/L Cd and the mixture of 50 μg/L VRP + 50 μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0 μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.</div>
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<s0>In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2 h at 50 μg/L VRP, 5.0 μg/L Cd, the mixture of 50 μg/L VRP + 5.0 μg/L Cd, 50 μg/L Cd and the mixture of 50 μg/L VRP + 50 μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0 μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.</s0>
</fC01>
<fC02 i1="01" i2="X">
<s0>002A14D05A</s0>
</fC02>
<fC02 i1="02" i2="X">
<s0>002A15B</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE">
<s0>Calcium</s0>
<s2>NC</s2>
<s2>FR</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG">
<s0>Calcium</s0>
<s2>NC</s2>
<s2>FR</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA">
<s0>Calcio</s0>
<s2>NC</s2>
<s2>FR</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Cadmium</s0>
<s2>NC</s2>
<s2>FX</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Cadmium</s0>
<s2>NC</s2>
<s2>FX</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Cadmio</s0>
<s2>NC</s2>
<s2>FX</s2>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Stress</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Stress</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Estrés</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Spermatozoïde</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Spermatozoa</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Espermatozoide</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Russe</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Russian</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Ruso</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Motilité</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>Motility</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>Motilidad</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE">
<s0>Antioxydant</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Antioxidant</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
<s0>Antioxidante</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE">
<s0>Milieu aquatique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG">
<s0>Aquatic environment</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
<s0>Medio acuático</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE">
<s0>Ecotoxicologie</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG">
<s0>Ecotoxicology</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA">
<s0>Ecotoxicología</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE">
<s0>Toxicité</s0>
<s5>23</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG">
<s0>Toxicity</s0>
<s5>23</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA">
<s0>Toxicidad</s0>
<s5>23</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE">
<s0>Polluant</s0>
<s5>24</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG">
<s0>Pollutant</s0>
<s5>24</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA">
<s0>Contaminante</s0>
<s5>24</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE">
<s0>Vérapamil</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>41</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG">
<s0>Verapamil</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>41</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA">
<s0>Verapamilo</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>41</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE">
<s0>Acipenser</s0>
<s4>INC</s4>
<s5>64</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Métal lourd</s0>
<s5>17</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Heavy metal</s0>
<s5>17</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Metal pesado</s0>
<s5>17</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Acipenseridae</s0>
<s4>INC</s4>
<s5>70</s5>
</fC07>
<fN21>
<s1>341</s1>
</fN21>
<fN44 i1="01">
<s1>OTO</s1>
</fN44>
<fN82>
<s1>OTO</s1>
</fN82>
</pA>
</standard>
<server>
<NO>PASCAL 10-0510437 INIST</NO>
<ET>Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii</ET>
<AU>LI (Zhi-Hua); PING LI; RODINA (Marek); RANDAK (Tomas)</AU>
<AF>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II/389 25 Vodnany/Tchèque, République (1 aut., 2 aut., 3 aut., 4 aut.); Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences/Jingzhou 434000/Chine (1 aut., 2 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Aquatic toxicology; ISSN 0166-445X; Coden AQTODG; Pays-Bas; Da. 2010; Vol. 100; No. 4; Pp. 373-375; Bibl. 1/4 p.</SO>
<LA>Anglais</LA>
<EA>In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2 h at 50 μg/L VRP, 5.0 μg/L Cd, the mixture of 50 μg/L VRP + 5.0 μg/L Cd, 50 μg/L Cd and the mixture of 50 μg/L VRP + 50 μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0 μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.</EA>
<CC>002A14D05A; 002A15B</CC>
<FD>Calcium; Cadmium; Stress; Spermatozoïde; Russe; Motilité; Antioxydant; Milieu aquatique; Ecotoxicologie; Toxicité; Polluant; Vérapamil; Acipenser</FD>
<FG>Métal lourd; Pisces; Vertebrata; Acipenseridae</FG>
<ED>Calcium; Cadmium; Stress; Spermatozoa; Russian; Motility; Antioxidant; Aquatic environment; Ecotoxicology; Toxicity; Pollutant; Verapamil</ED>
<EG>Heavy metal; Pisces; Vertebrata</EG>
<SD>Calcio; Cadmio; Estrés; Espermatozoide; Ruso; Motilidad; Antioxidante; Medio acuático; Ecotoxicología; Toxicidad; Contaminante; Verapamilo</SD>
<LO>INIST-18841.354000192532030090</LO>
<ID>10-0510437</ID>
</server>
</inist>
</record>

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