Effects of chlorpyrifos on in vitro sex steroid production and thyroid follicular development in adult and larval Lake Sturgeon, Acipenser fulvescens.
Identifieur interne : 000628 ( Ncbi/Curation ); précédent : 000627; suivant : 000629Effects of chlorpyrifos on in vitro sex steroid production and thyroid follicular development in adult and larval Lake Sturgeon, Acipenser fulvescens.
Auteurs : Catherine Brandt [Canada] ; Duncan C. Burnett [Canada] ; Liane Arcinas [Canada] ; Vince Palace [Canada] ; W. Gary Anderson [Canada]Source :
- Chemosphere [ 1879-1298 ] ; 2015.
English descriptors
- KwdEn :
- Animals, Chlorpyrifos (toxicity), Estradiol (metabolism), Female, Fishes (growth & development), Fishes (metabolism), Insecticides (toxicity), Larva (drug effects), Larva (growth & development), Larva (metabolism), Male, Ovary (drug effects), Ovary (metabolism), Testis (drug effects), Testis (metabolism), Testosterone (metabolism), Thyroid Gland (drug effects), Thyroid Gland (growth & development).
- MESH :
- chemical , metabolism : Estradiol, Testosterone.
- chemical , toxicity : Chlorpyrifos, Insecticides.
- drug effects : Larva, Ovary, Testis, Thyroid Gland.
- growth & development : Fishes, Larva, Thyroid Gland.
- metabolism : Fishes, Larva, Ovary, Testis.
- Animals, Female, Male.
Abstract
Chlorpyrifos is a widely used organophosphate pesticide that has previously been shown to enter waterways in biologically relevant concentrations and has the potential to disrupt both thyroid hormone and sex steroid biosynthesis in vertebrates. Because gonadal maturation and larval development in Lake Sturgeon, Acipenser fulvescens, potentially coincide with the application of chlorpyrifos we examined the effects of chlorpyrifos on both thyroid follicular development in larval Lake Sturgeon, and sex hormone synthesis in adult Lake Sturgeon. For the first time, the present study reports steroidogenesis from testicular and ovarian tissue in Lake Sturgeon using an established in vitro bioassay. Furthermore, incubating gonad tissue with 5, 500 or 2000ngmL(-1) chlorpyrifos revealed an inhibitory effect on testosterone synthesis in both testicular (control, 40.29pgmg(-1) tissue wet weight(-1)h(-1) compared to experimental, 21.84pgmg(-1) tissue wet weight(-1)h(-1)) and ovarian (control, 33.83pgmg(-1) tissue wet weight(-1)h(-1) compared to experimental, 15.19pgmg(-1) tissue wet weight(-1)h(-1)) tissue. In a second series of experiments, larval Lake Sturgeon were exposed to equivalent concentrations of chlorpyrifos as above for 10days (d) between hatch and the onset of exogenous feeding. Larvae from each treatment group were raised until 67days post hatch (dph) and growth rates were compared alongside key indicators of thyroid follicle growth. Chlorpyrifos treatment had no effect on the measured indicators of thyroid follicular development.
DOI: 10.1016/j.chemosphere.2015.03.031
PubMed: 25855011
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pubmed:25855011Le document en format XML
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Burnett</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, 150 Sifton Road, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; North/South Consultants Inc., 83 Scurfield Boulevard, Winnipeg R3Y 1G4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biological Sciences, 150 Sifton Road, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; North/South Consultants Inc., 83 Scurfield Boulevard, Winnipeg R3Y 1G4</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Arcinas, Liane" sort="Arcinas, Liane" uniqKey="Arcinas L" first="Liane" last="Arcinas">Liane Arcinas</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, 150 Sifton Road, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biological Sciences, 150 Sifton Road, University of Manitoba, Winnipeg, MB R3T 2N2</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Palace, Vince" sort="Palace, Vince" uniqKey="Palace V" first="Vince" last="Palace">Vince Palace</name><affiliation wicri:level="1"><nlm:affiliation>Stantec Consultants Ltd., 603-386 Broadway Ave, Winnipeg, MB R3C 3R6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Stantec Consultants Ltd., 603-386 Broadway Ave, Winnipeg, MB R3C 3R6</wicri:regionArea><wicri:noRegion>MB R3C 3R6</wicri:noRegion></affiliation></author><author><name sortKey="Gary Anderson, W" sort="Gary Anderson, W" uniqKey="Gary Anderson W" first="W" last="Gary Anderson">W. Gary Anderson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, 150 Sifton Road, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. 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Because gonadal maturation and larval development in Lake Sturgeon, Acipenser fulvescens, potentially coincide with the application of chlorpyrifos we examined the effects of chlorpyrifos on both thyroid follicular development in larval Lake Sturgeon, and sex hormone synthesis in adult Lake Sturgeon. For the first time, the present study reports steroidogenesis from testicular and ovarian tissue in Lake Sturgeon using an established in vitro bioassay. Furthermore, incubating gonad tissue with 5, 500 or 2000ngmL(-1) chlorpyrifos revealed an inhibitory effect on testosterone synthesis in both testicular (control, 40.29pgmg(-1) tissue wet weight(-1)h(-1) compared to experimental, 21.84pgmg(-1) tissue wet weight(-1)h(-1)) and ovarian (control, 33.83pgmg(-1) tissue wet weight(-1)h(-1) compared to experimental, 15.19pgmg(-1) tissue wet weight(-1)h(-1)) tissue. In a second series of experiments, larval Lake Sturgeon were exposed to equivalent concentrations of chlorpyrifos as above for 10days (d) between hatch and the onset of exogenous feeding. Larvae from each treatment group were raised until 67days post hatch (dph) and growth rates were compared alongside key indicators of thyroid follicle growth. Chlorpyrifos treatment had no effect on the measured indicators of thyroid follicular development.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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