Isolation and transplantation of sturgeon early-stage germ cells.
Identifieur interne : 000122 ( PubMed/Checkpoint ); précédent : 000121; suivant : 000123Isolation and transplantation of sturgeon early-stage germ cells.
Auteurs : Martin Pšeni Ka [République tchèque] ; Taiju Saito [République tchèque] ; Zuzana Linhartová [République tchèque] ; Ievgeniia Gazo [République tchèque]Source :
- Theriogenology [ 1879-3231 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- growth & development : Fishes.
- physiology : Fishes, Ovary, Testis.
- transplantation : Germ Cells.
- Animals, Female, Male, Sexual Maturation.
Abstract
We report, for the first time, a series of baseline techniques comprising isolation and transplantation of female and male early-stage germ cells in sturgeon to generate a germline chimera as a potential tool for surrogate reproduction and gene banking. Cells were dissociated from testis, characterized by mostly spermatogonia, and from ovary, exclusively comprising oogonia and previtellogenic oocytes, of Acipenser baerii, using 0.3% trypsin (2 hours, 23 °C) dissolved in PBS, isotonic with blood plasma. The dissociated germ cells were sorted by Percoll gradient centrifugation followed by immunolabeling with germ cell-specific vasa antibody DDX4, while 10% to 30% Percoll solution contained 79.4% and 70.8% labeled testicular and ovarian cells. Sorted germ cells were transplanted into a cavity close to a presumptive genital ridge of newly hatched heterospecific Acipenser ruthenus larvae with fluorescein isothiocyanate-labeled endogenous primordial germ cells. The transplanted germ cells were randomly distributed in the body cavity through 30-day posttransplantation (dpt). Subsequently, the cells were organized into genital ridges 50 dpt and proliferated 90 dpt. The number of both transplanted and endogenous germ cells significantly increased from 18.1, 22.2, and 29.1 (30 dpt) to 108.5, 90.8, and 118.5 (90 dpt) in ovarian, testicular, and endogenous germ cells, respectively (P < 0.05). The efficiency of transplantation was 60% (counted 90 dpt).
DOI: 10.1016/j.theriogenology.2014.12.010
PubMed: 25559841
Affiliations:
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Electronic address: psenicka@frov.jcu.cz.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea><wicri:noRegion>Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Saito, Taiju" sort="Saito, Taiju" uniqKey="Saito T" first="Taiju" last="Saito">Taiju Saito</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea><wicri:noRegion>Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Linhartova, Zuzana" sort="Linhartova, Zuzana" uniqKey="Linhartova Z" first="Zuzana" last="Linhartová">Zuzana Linhartová</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea><wicri:noRegion>Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Gazo, Ievgeniia" sort="Gazo, Ievgeniia" uniqKey="Gazo I" first="Ievgeniia" last="Gazo">Ievgeniia Gazo</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany</wicri:regionArea><wicri:noRegion>Vodňany</wicri:noRegion></affiliation></author></analytic><series><title level="j">Theriogenology</title><idno type="eISSN">1879-3231</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Female</term><term>Fishes (growth & development)</term><term>Fishes (physiology)</term><term>Germ Cells (transplantation)</term><term>Male</term><term>Ovary (physiology)</term><term>Sexual Maturation</term><term>Testis (physiology)</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term><term>Ovary</term><term>Testis</term></keywords><keywords scheme="MESH" qualifier="transplantation" xml:lang="en"><term>Germ Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Male</term><term>Sexual Maturation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report, for the first time, a series of baseline techniques comprising isolation and transplantation of female and male early-stage germ cells in sturgeon to generate a germline chimera as a potential tool for surrogate reproduction and gene banking. Cells were dissociated from testis, characterized by mostly spermatogonia, and from ovary, exclusively comprising oogonia and previtellogenic oocytes, of Acipenser baerii, using 0.3% trypsin (2 hours, 23 °C) dissolved in PBS, isotonic with blood plasma. The dissociated germ cells were sorted by Percoll gradient centrifugation followed by immunolabeling with germ cell-specific vasa antibody DDX4, while 10% to 30% Percoll solution contained 79.4% and 70.8% labeled testicular and ovarian cells. Sorted germ cells were transplanted into a cavity close to a presumptive genital ridge of newly hatched heterospecific Acipenser ruthenus larvae with fluorescein isothiocyanate-labeled endogenous primordial germ cells. The transplanted germ cells were randomly distributed in the body cavity through 30-day posttransplantation (dpt). Subsequently, the cells were organized into genital ridges 50 dpt and proliferated 90 dpt. The number of both transplanted and endogenous germ cells significantly increased from 18.1, 22.2, and 29.1 (30 dpt) to 108.5, 90.8, and 118.5 (90 dpt) in ovarian, testicular, and endogenous germ cells, respectively (P < 0.05). The efficiency of transplantation was 60% (counted 90 dpt).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25559841</PMID><DateCreated><Year>2015</Year><Month>03</Month><Day>03</Day></DateCreated><DateCompleted><Year>2015</Year><Month>12</Month><Day>01</Day></DateCompleted><DateRevised><Year>2015</Year><Month>03</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-3231</ISSN><JournalIssue CitedMedium="Internet"><Volume>83</Volume><Issue>6</Issue><PubDate><Year>2015</Year><Month>Apr</Month><Day>01</Day></PubDate></JournalIssue><Title>Theriogenology</Title><ISOAbbreviation>Theriogenology</ISOAbbreviation></Journal><ArticleTitle>Isolation and transplantation of sturgeon early-stage germ cells.</ArticleTitle><Pagination><MedlinePgn>1085-92</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.theriogenology.2014.12.010</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0093-691X(14)00685-2</ELocationID><Abstract><AbstractText>We report, for the first time, a series of baseline techniques comprising isolation and transplantation of female and male early-stage germ cells in sturgeon to generate a germline chimera as a potential tool for surrogate reproduction and gene banking. Cells were dissociated from testis, characterized by mostly spermatogonia, and from ovary, exclusively comprising oogonia and previtellogenic oocytes, of Acipenser baerii, using 0.3% trypsin (2 hours, 23 °C) dissolved in PBS, isotonic with blood plasma. The dissociated germ cells were sorted by Percoll gradient centrifugation followed by immunolabeling with germ cell-specific vasa antibody DDX4, while 10% to 30% Percoll solution contained 79.4% and 70.8% labeled testicular and ovarian cells. Sorted germ cells were transplanted into a cavity close to a presumptive genital ridge of newly hatched heterospecific Acipenser ruthenus larvae with fluorescein isothiocyanate-labeled endogenous primordial germ cells. The transplanted germ cells were randomly distributed in the body cavity through 30-day posttransplantation (dpt). Subsequently, the cells were organized into genital ridges 50 dpt and proliferated 90 dpt. The number of both transplanted and endogenous germ cells significantly increased from 18.1, 22.2, and 29.1 (30 dpt) to 108.5, 90.8, and 118.5 (90 dpt) in ovarian, testicular, and endogenous germ cells, respectively (P < 0.05). The efficiency of transplantation was 60% (counted 90 dpt).</AbstractText><CopyrightInformation>Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pšenička</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech Republic. Electronic address: psenicka@frov.jcu.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>Taiju</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Linhartová</LastName><ForeName>Zuzana</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gazo</LastName><ForeName>Ievgeniia</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>12</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Theriogenology</MedlineTA><NlmUniqueID>0421510</NlmUniqueID><ISSNLinking>0093-691X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005854" MajorTopicYN="N">Germ Cells</DescriptorName><QualifierName UI="Q000637" MajorTopicYN="Y">transplantation</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010053" MajorTopicYN="N">Ovary</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012741" MajorTopicYN="N">Sexual Maturation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013737" MajorTopicYN="N">Testis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Germ cell</Keyword><Keyword MajorTopicYN="N">Germline chimera</Keyword><Keyword MajorTopicYN="N">Oogonium</Keyword><Keyword MajorTopicYN="N">Spermatogonium</Keyword><Keyword MajorTopicYN="N">Sturgeon</Keyword><Keyword MajorTopicYN="N">Vas</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>12</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>1</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>1</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25559841</ArticleId><ArticleId IdType="pii">S0093-691X(14)00685-2</ArticleId><ArticleId IdType="doi">10.1016/j.theriogenology.2014.12.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République tchèque</li></country></list><tree><country name="République tchèque"><noRegion><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name></noRegion><name sortKey="Gazo, Ievgeniia" sort="Gazo, Ievgeniia" uniqKey="Gazo I" first="Ievgeniia" last="Gazo">Ievgeniia Gazo</name><name sortKey="Linhartova, Zuzana" sort="Linhartova, Zuzana" uniqKey="Linhartova Z" first="Zuzana" last="Linhartová">Zuzana Linhartová</name><name sortKey="Saito, Taiju" sort="Saito, Taiju" uniqKey="Saito T" first="Taiju" last="Saito">Taiju Saito</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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