Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery.
Identifieur interne : 000285 ( Ncbi/Checkpoint ); précédent : 000284; suivant : 000286Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery.
Auteurs : Matthew C. Hale [États-Unis] ; Cory R. Mccormick ; James R. Jackson ; J Andrew DewoodySource :
- BMC genomics [ 1471-2164 ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- genetics : Fishes.
- metabolism : Gonads.
- methods : Gene Expression Profiling, Sequence Analysis, DNA.
- Animals, Expressed Sequence Tags, Female, Gene Library, Male, Pilot Projects, Polymorphism, Single Nucleotide.
Abstract
Next-generation sequencing technologies have been applied most often to model organisms or species closely related to a model. However, these methods have the potential to be valuable in many wild organisms, including those of conservation concern. We used Roche 454 pyrosequencing to characterize gene expression in polyploid lake sturgeon (Acipenser fulvescens) gonads.
DOI: 10.1186/1471-2164-10-203
PubMed: 19402907
Affiliations:
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pubmed:19402907Le document en format XML
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<front><div type="abstract" xml:lang="en">Next-generation sequencing technologies have been applied most often to model organisms or species closely related to a model. However, these methods have the potential to be valuable in many wild organisms, including those of conservation concern. We used Roche 454 pyrosequencing to characterize gene expression in polyploid lake sturgeon (Acipenser fulvescens) gonads.</div>
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