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Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery.

Identifieur interne : 000285 ( Ncbi/Curation ); précédent : 000284; suivant : 000286

Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery.

Auteurs : Matthew C. Hale [États-Unis] ; Cory R. Mccormick ; James R. Jackson ; J Andrew Dewoody

Source :

RBID : pubmed:19402907

English descriptors

Abstract

Next-generation sequencing technologies have been applied most often to model organisms or species closely related to a model. However, these methods have the potential to be valuable in many wild organisms, including those of conservation concern. We used Roche 454 pyrosequencing to characterize gene expression in polyploid lake sturgeon (Acipenser fulvescens) gonads.

DOI: 10.1186/1471-2164-10-203
PubMed: 19402907

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pubmed:19402907

Le document en format XML

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<term>Gene Library</term>
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<term>Polymorphism, Single Nucleotide</term>
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<div type="abstract" xml:lang="en">Next-generation sequencing technologies have been applied most often to model organisms or species closely related to a model. However, these methods have the potential to be valuable in many wild organisms, including those of conservation concern. We used Roche 454 pyrosequencing to characterize gene expression in polyploid lake sturgeon (Acipenser fulvescens) gonads.</div>
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