L'Hormone gonadotrope hypophysaire d'un poisson chondrostéen, l'esturgeon ( Acipenser stellatus pall.) I. Purification
Identifieur interne : 002367 ( Main/Merge ); précédent : 002366; suivant : 002368L'Hormone gonadotrope hypophysaire d'un poisson chondrostéen, l'esturgeon ( Acipenser stellatus pall.) I. Purification
Auteurs : E. Burzawa-Gerard [France] ; B. F. Goncharov [France, Russie] ; Y. A. Fontaine [France]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1975.
Abstract
Abstract: Pituitary glands of sturgeon (Acipenser stellatus) have been extracted and fractionated by alcoholic percolation, gel filtration on Sephadex G 100, and chromatography on DEAE-C. Biological activity was assayed in vivo on frog spermiation and in vitro on the maturation of oocytes from amphibians and sturgeon. Only one gonadotropic fraction—adsorbed on DEAE-C in glycinate buffer pH 9.4, 0.02 M—was obtained. Its biological activity was about 12 times that of the pituitary acetonic powder both on spermiation and on oocyte maturation. This material (aci-GTH) was slightly more active than carp GTH on frog spermiation.
Url:
DOI: 10.1016/0016-6480(75)90197-5
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Biological activity was assayed in vivo on frog spermiation and in vitro on the maturation of oocytes from amphibians and sturgeon. Only one gonadotropic fraction—adsorbed on DEAE-C in glycinate buffer pH 9.4, 0.02 M—was obtained. Its biological activity was about 12 times that of the pituitary acetonic powder both on spermiation and on oocyte maturation. This material (aci-GTH) was slightly more active than carp GTH on frog spermiation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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