L'hormone gonadotrope hypophysaire d'un poisson chondrostéen, l'esturgeon ( Acipenser stellatus Pall.). II. Propriétés biochimiques
Identifieur interne : 002366 ( Main/Merge ); précédent : 002365; suivant : 002367L'hormone gonadotrope hypophysaire d'un poisson chondrostéen, l'esturgeon ( Acipenser stellatus Pall.). II. Propriétés biochimiques
Auteurs : E. Burzawa-Gerard [France] ; B. F. Goncharov [France, Russie] ; Y. A. Fontaine [France]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1975.
Abstract
Abstract: Biochemical properties of the aci-GTH prepared by gel filtration and DEAE-C chromatography were studied. This material had a sedimentation coefficient of 2.4–2.8. In analytical electrophoresis acrylamide gel at pH 8.9 it gave two main bands, a and b (Rf = 0.27 and 0.31) both of them active in the frog spermiation test. Preparative acrylamide electrophoresis led to the separation of two active fractions, of which one contained only band a. Amino acid composition of this fraction was determined. Dissociation agents strikingly modified the properties of aci-GTH. Sedimentation rate was decreased to a value of 1.5. Biological activity was less than one-tenth of that of the native hormone. Analytical electrophoresis showed the formation, from band a, of two more acidic bands (Rf = 0.53 and 0.59). These results strongly indicate that aci-GTH was, in these conditions, dissociated into two subunits. Aci-GTH is different from carp GTH in amino acid composition, electrophoretic behavior, and level of the equilibrium between the hormone and its subunits in several experimental conditions.
Url:
DOI: 10.1016/0016-6480(75)90198-7
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000440
- to stream Istex, to step Curation: 000439
- to stream Istex, to step Checkpoint: 001476
Links to Exploration step
ISTEX:58AA1489D3849E00C12DF607BA328EDC00682119Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="fr">L'hormone gonadotrope hypophysaire d'un poisson chondrostéen, l'esturgeon ( Acipenser stellatus Pall.). II. Propriétés biochimiques</title><author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gerard">E. Burzawa-Gerard</name></author><author><name sortKey="Goncharov, B F" sort="Goncharov, B F" uniqKey="Goncharov B" first="B. F." last="Goncharov">B. F. Goncharov</name></author><author><name sortKey="Fontaine, Y A" sort="Fontaine, Y A" uniqKey="Fontaine Y" first="Y. A." last="Fontaine">Y. A. Fontaine</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:58AA1489D3849E00C12DF607BA328EDC00682119</idno><date when="1975" year="1975">1975</date><idno type="doi">10.1016/0016-6480(75)90198-7</idno><idno type="url">https://api.istex.fr/document/58AA1489D3849E00C12DF607BA328EDC00682119/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000440</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000440</idno><idno type="wicri:Area/Istex/Curation">000439</idno><idno type="wicri:Area/Istex/Checkpoint">001476</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001476</idno><idno type="wicri:doubleKey">0016-6480:1975:Burzawa Gerard E:l:hormone:gonadotrope</idno><idno type="wicri:Area/Main/Merge">002366</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="fr">L'hormone gonadotrope hypophysaire d'un poisson chondrostéen, l'esturgeon ( Acipenser stellatus Pall.). II. Propriétés biochimiques</title><author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gerard">E. Burzawa-Gerard</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie du Muséum d'Histoire Naturelle, 7, rue Cuvier, 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Endocrinologie comparée associé au C.N.R.S., 7, rue Cuvier, 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Goncharov, B F" sort="Goncharov, B F" uniqKey="Goncharov B" first="B. F." last="Goncharov">B. F. Goncharov</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie du Muséum d'Histoire Naturelle, 7, rue Cuvier, 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Endocrinologie comparée associé au C.N.R.S., 7, rue Cuvier, 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Russie</country><wicri:regionArea>2 Adresse actuelle: Institute of Developmental Biology, Academy of Sciences USSR, 26 St. Vavilov, Moscou 117 334</wicri:regionArea><wicri:noRegion>Moscou 117 334</wicri:noRegion></affiliation></author><author><name sortKey="Fontaine, Y A" sort="Fontaine, Y A" uniqKey="Fontaine Y" first="Y. A." last="Fontaine">Y. A. Fontaine</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie du Muséum d'Histoire Naturelle, 7, rue Cuvier, 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Endocrinologie comparée associé au C.N.R.S., 7, rue Cuvier, 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="ISSN">0016-6480</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1975">1975</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="296">296</biblScope><biblScope unit="page" to="304">304</biblScope></imprint><idno type="ISSN">0016-6480</idno></series><idno type="istex">58AA1489D3849E00C12DF607BA328EDC00682119</idno><idno type="DOI">10.1016/0016-6480(75)90198-7</idno><idno type="PII">0016-6480(75)90198-7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0016-6480</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="fr">fr</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Biochemical properties of the aci-GTH prepared by gel filtration and DEAE-C chromatography were studied. This material had a sedimentation coefficient of 2.4–2.8. In analytical electrophoresis acrylamide gel at pH 8.9 it gave two main bands, a and b (Rf = 0.27 and 0.31) both of them active in the frog spermiation test. Preparative acrylamide electrophoresis led to the separation of two active fractions, of which one contained only band a. Amino acid composition of this fraction was determined. Dissociation agents strikingly modified the properties of aci-GTH. Sedimentation rate was decreased to a value of 1.5. Biological activity was less than one-tenth of that of the native hormone. Analytical electrophoresis showed the formation, from band a, of two more acidic bands (Rf = 0.53 and 0.59). These results strongly indicate that aci-GTH was, in these conditions, dissociated into two subunits. Aci-GTH is different from carp GTH in amino acid composition, electrophoretic behavior, and level of the equilibrium between the hormone and its subunits in several experimental conditions.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002366 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 002366 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:58AA1489D3849E00C12DF607BA328EDC00682119
|texte= L'hormone gonadotrope hypophysaire d'un poisson chondrostéen, l'esturgeon ( Acipenser stellatus Pall.). II. Propriétés biochimiques
}}
| This area was generated with Dilib version V0.6.27. |  |