Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting
Identifieur interne : 001697 ( Main/Merge ); précédent : 001696; suivant : 001698Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting
Auteurs : Ingo Jenneckens [Allemagne] ; Andreas Müller-Belecke [Allemagne] ; Gabriele Hörstgen-Schwark [Allemagne] ; Johann-Nikolaus Meyer [Allemagne]Source :
- Aquaculture [ 0044-8486 ] ; 1999.
Abstract
In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (Oreochromis niloticus). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.
Url:
DOI: 10.1016/S0044-8486(98)00462-1
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type="city">Göttingen</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="ISSN">0044-8486</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">173</biblScope><biblScope unit="issue">1–4</biblScope><biblScope unit="page" from="377">377</biblScope><biblScope unit="page" to="388">388</biblScope></imprint><idno type="ISSN">0044-8486</idno></series><idno type="istex">095C323C564FFB91A0221CF0830A47AE19511A18</idno><idno type="DOI">10.1016/S0044-8486(98)00462-1</idno><idno type="PII">S0044-8486(98)00462-1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0044-8486</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (Oreochromis niloticus). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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