Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting
Identifieur interne : 000822 ( Istex/Corpus ); précédent : 000821; suivant : 000823Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting
Auteurs : Ingo Jenneckens ; Andreas Müller-Belecke ; Gabriele Hörstgen-Schwark ; Johann-Nikolaus MeyerSource :
- Aquaculture [ 0044-8486 ] ; 1999.
Abstract
In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (Oreochromis niloticus). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.
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DOI: 10.1016/S0044-8486(98)00462-1
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting</title><author><name sortKey="Jenneckens, Ingo" sort="Jenneckens, Ingo" uniqKey="Jenneckens I" first="Ingo" last="Jenneckens">Ingo Jenneckens</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Muller Belecke, Andreas" sort="Muller Belecke, Andreas" uniqKey="Muller Belecke A" first="Andreas" last="Müller-Belecke">Andreas Müller-Belecke</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Horstgen Schwark, Gabriele" sort="Horstgen Schwark, Gabriele" uniqKey="Horstgen Schwark G" first="Gabriele" last="Hörstgen-Schwark">Gabriele Hörstgen-Schwark</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Meyer, Johann Nikolaus" sort="Meyer, Johann Nikolaus" uniqKey="Meyer J" first="Johann-Nikolaus" last="Meyer">Johann-Nikolaus Meyer</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:095C323C564FFB91A0221CF0830A47AE19511A18</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1016/S0044-8486(98)00462-1</idno><idno type="url">https://api.istex.fr/document/095C323C564FFB91A0221CF0830A47AE19511A18/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000822</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000822</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting</title><author><name sortKey="Jenneckens, Ingo" sort="Jenneckens, Ingo" uniqKey="Jenneckens I" first="Ingo" last="Jenneckens">Ingo Jenneckens</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Muller Belecke, Andreas" sort="Muller Belecke, Andreas" uniqKey="Muller Belecke A" first="Andreas" last="Müller-Belecke">Andreas Müller-Belecke</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Horstgen Schwark, Gabriele" sort="Horstgen Schwark, Gabriele" uniqKey="Horstgen Schwark G" first="Gabriele" last="Hörstgen-Schwark">Gabriele Hörstgen-Schwark</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Meyer, Johann Nikolaus" sort="Meyer, Johann Nikolaus" uniqKey="Meyer J" first="Johann-Nikolaus" last="Meyer">Johann-Nikolaus Meyer</name><affiliation><mods:affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="ISSN">0044-8486</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">173</biblScope><biblScope unit="issue">1–4</biblScope><biblScope unit="page" from="377">377</biblScope><biblScope unit="page" to="388">388</biblScope></imprint><idno type="ISSN">0044-8486</idno></series><idno type="istex">095C323C564FFB91A0221CF0830A47AE19511A18</idno><idno type="DOI">10.1016/S0044-8486(98)00462-1</idno><idno type="PII">S0044-8486(98)00462-1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0044-8486</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (Oreochromis niloticus). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Ingo Jenneckens</name><affiliations><json:string>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Andreas Müller-Belecke</name><affiliations><json:string>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Gabriele Hörstgen-Schwark</name><affiliations><json:string>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Johann-Nikolaus Meyer</name><affiliations><json:string>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Oreochromis niloticus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Genetic marker</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Clones</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DNA fingerprinting</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (Oreochromis niloticus). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.</abstract><qualityIndicators><score>5.621</score><pdfVersion>1.2</pdfVersion><pdfPageSize>435 x 643 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>1362</abstractCharCount><pdfWordCount>3173</pdfWordCount><pdfCharCount>20078</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>204</abstractWordCount></qualityIndicators><title>Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting</title><pii><json:string>S0044-8486(98)00462-1</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>173</volume><pii><json:string>S0044-8486(00)X0086-5</json:string></pii><pages><last>388</last><first>377</first></pages><issn><json:string>0044-8486</json:string></issn><issue>1–4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Aquaculture</title><publicationDate>1999</publicationDate></host><categories><wos><json:string>science</json:string><json:string>marine & freshwater biology</json:string><json:string>fisheries</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>fisheries</json:string></scienceMetrix></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S0044-8486(98)00462-1</json:string></doi><id>095C323C564FFB91A0221CF0830A47AE19511A18</id><score>0.041468624</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/095C323C564FFB91A0221CF0830A47AE19511A18/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/095C323C564FFB91A0221CF0830A47AE19511A18/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/095C323C564FFB91A0221CF0830A47AE19511A18/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1999 Elsevier Science B.V.</p></availability><date>1999</date></publicationStmt><notesStmt><note type="content">Fig. 1: Multilocus DNA fingerprint pattern of the mitogyne (mit) clone founders with probe (GTG)5. M=molecular weight marker λ/HindIII. Lanes 2–7=HaeIII digested DNA, lanes 9–14=RsaI digested DNA.</note><note type="content">Fig. 2: Agarose gel electrophoresis of RAPD PCR amplification products obtained with primer OPH-03 of the male and the mitogyne (mit) clone founders. M=100 bp ladder.</note><note type="content">Fig. 3: Band pattern of simple sequence repeat-anchored PCR amplification products generated with primer (GT)7TC of the mitogyne (mit) clone founders. M=100 bp ladder.</note><note type="content">Fig. 4: Multilocus DNA fingerprint pattern generated with HaeIII and probe (GTG)5 of the male, mitogyne 26 (mit 26) and five of its gynogenetic offspring. M=molecular weight marker λ/HindIII. 1–5=gynogenetic offspring.</note><note type="content">Fig. 5: Agarose gel electrophoresis of RAPD PCR amplification products obtained with primer OPH-02 of the male, clone founder mit 77 and diploid control offspring. M=100 bp ladder. 1–10=diploid control offspring.</note><note type="content">Fig. 6: Agarose gel electrophoresis of RAPD PCR amplification products obtained with primer OPH-15 of the male, the mitogyne clone founder No. 26 (mit 26) and five of its gynogenetic offspring. M=100 bp ladder. 1–5=gynogenetic offspring. Informative paternal bands are indicated by an arrowhead.</note><note type="content">Table 1: Numbers of heterozygous female parents, their F1 mitogyne (mit) females (clone founders), numbers of produced and by DNA fingerprinting tested second generation meiogyne offspring within clonal lines as well as diploid controls</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting</title><author xml:id="author-1"><persName><forename type="first">Ingo</forename><surname>Jenneckens</surname></persName><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation></author><author xml:id="author-2"><persName><forename type="first">Andreas</forename><surname>Müller-Belecke</surname></persName><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation></author><author xml:id="author-3"><persName><forename type="first">Gabriele</forename><surname>Hörstgen-Schwark</surname></persName><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation></author><author xml:id="author-4"><persName><forename type="first">Johann-Nikolaus</forename><surname>Meyer</surname></persName><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation></author></analytic><monogr><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="pISSN">0044-8486</idno><idno type="PII">S0044-8486(00)X0086-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">173</biblScope><biblScope unit="issue">1–4</biblScope><biblScope unit="page" from="377">377</biblScope><biblScope unit="page" to="388">388</biblScope></imprint></monogr><idno type="istex">095C323C564FFB91A0221CF0830A47AE19511A18</idno><idno type="DOI">10.1016/S0044-8486(98)00462-1</idno><idno type="PII">S0044-8486(98)00462-1</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (Oreochromis niloticus). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Oreochromis niloticus</term></item><item><term>Genetic marker</term></item><item><term>Clones</term></item><item><term>DNA fingerprinting</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/095C323C564FFB91A0221CF0830A47AE19511A18/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity><istex:entity SYSTEM="gr5" NDATA="IMAGE" name="gr5"></istex:entity><istex:entity SYSTEM="gr6" NDATA="IMAGE" name="gr6"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>AQUA</jid><aid>60782</aid><ce:pii>S0044-8486(98)00462-1</ce:pii><ce:doi>10.1016/S0044-8486(98)00462-1</ce:doi><ce:copyright year="1999" type="full-transfer">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Proof of the successful development of Nile tilapia (<ce:italic>Oreochromis niloticus</ce:italic>) clones by DNA fingerprinting</ce:title><ce:author-group><ce:author><ce:given-name>Ingo</ce:given-name><ce:surname>Jenneckens</ce:surname><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>Andreas</ce:given-name><ce:surname>Müller-Belecke</ce:surname></ce:author><ce:author><ce:given-name>Gabriele</ce:given-name><ce:surname>Hörstgen-Schwark</ce:surname></ce:author><ce:author><ce:given-name>Johann-Nikolaus</ce:given-name><ce:surname>Meyer</ce:surname></ce:author><ce:affiliation><ce:textfn>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +49-551-395629; Fax: +49-551-395587; E-mail: ijennec@gwdg.de</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="14" month="10" year="1998"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (<ce:italic>Oreochromis niloticus</ce:italic>). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Oreochromis niloticus</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Genetic marker</ce:text></ce:keyword><ce:keyword><ce:text>Clones</ce:text></ce:keyword><ce:keyword><ce:text>DNA fingerprinting</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Proof of the successful development of Nile tilapia ( Oreochromis niloticus ) clones by DNA fingerprinting</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Proof of the successful development of Nile tilapia (</title></titleInfo><name type="personal"><namePart type="given">Ingo</namePart><namePart type="family">Jenneckens</namePart><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation><description>Corresponding author. Tel.: +49-551-395629; Fax: +49-551-395587; E-mail: ijennec@gwdg.de</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Andreas</namePart><namePart type="family">Müller-Belecke</namePart><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gabriele</namePart><namePart type="family">Hörstgen-Schwark</namePart><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Johann-Nikolaus</namePart><namePart type="family">Meyer</namePart><affiliation>Institute for Animal Breeding and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, D-37075 Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">In a study described in this paper, three different DNA-based techniques were applied to check the success of gynogenesis in Nile tilapia (Oreochromis niloticus). Out of six potentially homozygous female mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. The aim of this investigation was to confirm the clonal status of the clone founders, the genetic uniformity of gynogenetic offspring from each clone founder and the genetic differences between clonal lines on DNA level. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened using the techniques of multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques were able to show that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD were used to demonstrate that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the used primers in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.</abstract><note type="content">Fig. 1: Multilocus DNA fingerprint pattern of the mitogyne (mit) clone founders with probe (GTG)5. M=molecular weight marker λ/HindIII. Lanes 2–7=HaeIII digested DNA, lanes 9–14=RsaI digested DNA.</note><note type="content">Fig. 2: Agarose gel electrophoresis of RAPD PCR amplification products obtained with primer OPH-03 of the male and the mitogyne (mit) clone founders. M=100 bp ladder.</note><note type="content">Fig. 3: Band pattern of simple sequence repeat-anchored PCR amplification products generated with primer (GT)7TC of the mitogyne (mit) clone founders. M=100 bp ladder.</note><note type="content">Fig. 4: Multilocus DNA fingerprint pattern generated with HaeIII and probe (GTG)5 of the male, mitogyne 26 (mit 26) and five of its gynogenetic offspring. M=molecular weight marker λ/HindIII. 1–5=gynogenetic offspring.</note><note type="content">Fig. 5: Agarose gel electrophoresis of RAPD PCR amplification products obtained with primer OPH-02 of the male, clone founder mit 77 and diploid control offspring. M=100 bp ladder. 1–10=diploid control offspring.</note><note type="content">Fig. 6: Agarose gel electrophoresis of RAPD PCR amplification products obtained with primer OPH-15 of the male, the mitogyne clone founder No. 26 (mit 26) and five of its gynogenetic offspring. M=100 bp ladder. 1–5=gynogenetic offspring. Informative paternal bands are indicated by an arrowhead.</note><note type="content">Table 1: Numbers of heterozygous female parents, their F1 mitogyne (mit) females (clone founders), numbers of produced and by DNA fingerprinting tested second generation meiogyne offspring within clonal lines as well as diploid controls</note><subject><genre>Keywords</genre><topic>Oreochromis niloticus</topic><topic>Genetic marker</topic><topic>Clones</topic><topic>DNA fingerprinting</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture</title></titleInfo><titleInfo type="abbreviated"><title>AQUA</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19990330</dateIssued></originInfo><identifier type="ISSN">0044-8486</identifier><identifier type="PII">S0044-8486(00)X0086-5</identifier><part><date>19990330</date><detail type="volume"><number>173</number><caption>vol.</caption></detail><detail type="issue"><number>1–4</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>498</end></extent><extent unit="pages"><start>377</start><end>388</end></extent></part></relatedItem><identifier type="istex">095C323C564FFB91A0221CF0830A47AE19511A18</identifier><identifier type="DOI">10.1016/S0044-8486(98)00462-1</identifier><identifier type="PII">S0044-8486(98)00462-1</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science B.V., ©1999</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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