Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions
Identifieur interne : 001451 ( Main/Merge ); précédent : 001450; suivant : 001452Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions
Auteurs : J. A. Lacey [États-Unis] ; K. J. Rodnick [États-Unis]Source :
- Journal of fish biology [ 0022-1112 ] ; 2002.
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- Pascal (Inist)
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Abstract
Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.
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Pascal:02-0466515Le document en format XML
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Rodnick</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1><s2>Pocatello, ID 83209-8007</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Idaho</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">02-0466515</idno><date when="2002">2002</date><idno type="stanalyst">PASCAL 02-0466515 INIST</idno><idno type="RBID">Pascal:02-0466515</idno><idno type="wicri:Area/PascalFrancis/Corpus">000313</idno><idno type="wicri:Area/PascalFrancis/Curation">000063</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000308</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000308</idno><idno type="wicri:doubleKey">0022-1112:2002:Lacey J:important:considerations:for</idno><idno type="wicri:Area/Main/Merge">001451</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions</title><author><name sortKey="Lacey, J A" sort="Lacey, J A" uniqKey="Lacey J" first="J. A." last="Lacey">J. A. Lacey</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1><s2>Pocatello, ID 83209-8007</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Idaho</region></placeName></affiliation></author><author><name sortKey="Rodnick, K J" sort="Rodnick, K J" uniqKey="Rodnick K" first="K. J." last="Rodnick">K. J. Rodnick</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1><s2>Pocatello, ID 83209-8007</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Idaho</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of fish biology</title><title level="j" type="abbreviated">J. fish biol.</title><idno type="ISSN">0022-1112</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of fish biology</title><title level="j" type="abbreviated">J. fish biol.</title><idno type="ISSN">0022-1112</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term><term>Blood</term><term>Ictalurus punctatus</term><term>Interspecific comparison</term><term>Measurement method</term><term>Methemoglobin</term><term>Oncorhynchus mykiss</term><term>Spectrophotometry</term><term>Tilapia nilotica</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Sang</term><term>Méthémoglobine</term><term>Spectrophotométrie</term><term>Méthode mesure</term><term>Comparaison interspécifique</term><term>Oncorhynchus mykiss</term><term>Ictalurus punctatus</term><term>Tilapia nilotica</term><term>Acipenser transmontanus</term><term>Tilapia zillii</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Idaho</li></region></list><tree><country name="États-Unis"><region name="Idaho"><name sortKey="Lacey, J A" sort="Lacey, J A" uniqKey="Lacey J" first="J. A." last="Lacey">J. A. Lacey</name></region><name sortKey="Rodnick, K J" sort="Rodnick, K J" uniqKey="Rodnick K" first="K. J." last="Rodnick">K. J. Rodnick</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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