EsturgeonV1, PascalFrancis, Corpus, bibRecord, 000313

Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions

Identifieur interne : 000313 ( PascalFrancis/Corpus ); précédent : 000312; suivant : 000314

Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions

Auteurs : J. A. Lacey ; K. J. Rodnick

Source :

Journal of fish biology [ 0022-1112 ] ; 2002.

RBID : Pascal:02-0466515

Descripteurs français

Pascal (Inist)
- Sang, Méthémoglobine, Spectrophotométrie, Méthode mesure, Comparaison interspécifique, Oncorhynchus mykiss, Ictalurus punctatus, Tilapia nilotica, Acipenser transmontanus, Tilapia zillii.

English descriptors

KwdEn :
- Acipenser transmontanus, Blood, Ictalurus punctatus, Interspecific comparison, Measurement method, Methemoglobin, Oncorhynchus mykiss, Spectrophotometry, Tilapia nilotica.

Abstract

Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0022-1112`
A02	`01`			`@0 JFIBA9`
A03		`1`		`@0 J. fish biol.`
A05				`@2 60`
A06				`@2 5`
A08	`01`	`1`	`ENG`	`@1 Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions`
A11	`01`	`1`		`@1 LACEY (J. A.)`
A11	`02`	`1`		`@1 RODNICK (K. J.)`
A14	`01`			`@1 Department of Biological Sciences, Idaho State University @2 Pocatello, ID 83209-8007 @3 USA @Z 1 aut. @Z 2 aut.`
A20				`@1 1155-1169`
A21				`@1 2002`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 14279 @5 354000104406760080`
A44				`@0 0000 @1 © 2002 INIST-CNRS. All rights reserved.`
A45				`@0 1 p.1/4`
A47	`01`	`1`		`@0 02-0466515`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of fish biology`
A66	`01`			`@0 GBR`
C01	`01`		`ENG`	@0 Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.
C02	`01`	`X`		`@0 002A21`
C03	`01`	`X`	`FRE`	`@0 Sang @5 01`
C03	`01`	`X`	`ENG`	`@0 Blood @5 01`
C03	`01`	`X`	`SPA`	`@0 Sangre @5 01`
C03	`02`	`X`	`FRE`	`@0 Méthémoglobine @5 02`
C03	`02`	`X`	`ENG`	`@0 Methemoglobin @5 02`
C03	`02`	`X`	`SPA`	`@0 Metahemoglobina @5 02`
C03	`03`	`X`	`FRE`	`@0 Spectrophotométrie @5 03`
C03	`03`	`X`	`ENG`	`@0 Spectrophotometry @5 03`
C03	`03`	`X`	`SPA`	`@0 Espectrofotometría @5 03`
C03	`04`	`X`	`FRE`	`@0 Méthode mesure @5 04`
C03	`04`	`X`	`ENG`	`@0 Measurement method @5 04`
C03	`04`	`X`	`SPA`	`@0 Método medida @5 04`
C03	`05`	`X`	`FRE`	`@0 Comparaison interspécifique @5 05`
C03	`05`	`X`	`ENG`	`@0 Interspecific comparison @5 05`
C03	`05`	`X`	`SPA`	`@0 Comparación interespecífica @5 05`
C03	`06`	`X`	`FRE`	`@0 Oncorhynchus mykiss @2 NS @5 55`
C03	`06`	`X`	`ENG`	`@0 Oncorhynchus mykiss @2 NS @5 55`
C03	`06`	`X`	`SPA`	`@0 Oncorhynchus mykiss @2 NS @5 55`
C03	`07`	`X`	`FRE`	`@0 Ictalurus punctatus @2 NS @5 56`
C03	`07`	`X`	`ENG`	`@0 Ictalurus punctatus @2 NS @5 56`
C03	`07`	`X`	`SPA`	`@0 Ictalurus punctatus @2 NS @5 56`
C03	`08`	`X`	`FRE`	`@0 Tilapia nilotica @2 NS @5 57`
C03	`08`	`X`	`ENG`	`@0 Tilapia nilotica @2 NS @5 57`
C03	`08`	`X`	`SPA`	`@0 Tilapia nilotica @2 NS @5 57`
C03	`09`	`X`	`FRE`	`@0 Acipenser transmontanus @2 NS @5 58`
C03	`09`	`X`	`ENG`	`@0 Acipenser transmontanus @2 NS @5 58`
C03	`09`	`X`	`SPA`	`@0 Acipenser transmontanus @2 NS @5 58`
C03	`10`	`X`	`FRE`	`@0 Tilapia zillii @2 NS @4 INC @5 65`
C07	`01`	`X`	`FRE`	`@0 Milieu aquatique @5 17`
C07	`01`	`X`	`ENG`	`@0 Aquatic environment @5 17`
C07	`01`	`X`	`SPA`	`@0 Medio acuático @5 17`
C07	`02`	`X`	`FRE`	`@0 Cichlidae @2 NS @5 29`
C07	`02`	`X`	`ENG`	`@0 Cichlidae @2 NS @5 29`
C07	`02`	`X`	`SPA`	`@0 Cichlidae @2 NS @5 29`
C07	`03`	`X`	`FRE`	`@0 Pisces @2 NS`
C07	`03`	`X`	`ENG`	`@0 Pisces @2 NS`
C07	`03`	`X`	`SPA`	`@0 Pisces @2 NS`
C07	`04`	`X`	`FRE`	`@0 Vertebrata @2 NS`
C07	`04`	`X`	`ENG`	`@0 Vertebrata @2 NS`
C07	`04`	`X`	`SPA`	`@0 Vertebrata @2 NS`
C07	`05`	`X`	`FRE`	`@0 Salmonidae @2 NS @5 60`
C07	`05`	`X`	`ENG`	`@0 Salmonidae @2 NS @5 60`
C07	`05`	`X`	`SPA`	`@0 Salmonidae @2 NS @5 60`
C07	`06`	`X`	`FRE`	`@0 Ictaluridae @2 NS @4 INC @5 71`
C07	`07`	`X`	`FRE`	`@0 Acipenseridae @2 NS @4 INC @5 73`
N21				`@1 273`
N82				`@1 PSI`

Format Inist (serveur)

NO :	PASCAL 02-0466515 INIST
ET :	Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions
AU :	LACEY (J. A.); RODNICK (K. J.)
AF :	Department of Biological Sciences, Idaho State University/Pocatello, ID 83209-8007/Etats-Unis (1 aut., 2 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of fish biology; ISSN 0022-1112; Coden JFIBA9; Royaume-Uni; Da. 2002; Vol. 60; No. 5; Pp. 1155-1169; Bibl. 1 p.1/4
LA :	Anglais
EA :	Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.
CC :	002A21
FD :	Sang; Méthémoglobine; Spectrophotométrie; Méthode mesure; Comparaison interspécifique; Oncorhynchus mykiss; Ictalurus punctatus; Tilapia nilotica; Acipenser transmontanus; Tilapia zillii
FG :	Milieu aquatique; Cichlidae; Pisces; Vertebrata; Salmonidae; Ictaluridae; Acipenseridae
ED :	Blood; Methemoglobin; Spectrophotometry; Measurement method; Interspecific comparison; Oncorhynchus mykiss; Ictalurus punctatus; Tilapia nilotica; Acipenser transmontanus
EG :	Aquatic environment; Cichlidae; Pisces; Vertebrata; Salmonidae
SD :	Sangre; Metahemoglobina; Espectrofotometría; Método medida; Comparación interespecífica; Oncorhynchus mykiss; Ictalurus punctatus; Tilapia nilotica; Acipenser transmontanus
LO :	INIST-14279.354000104406760080
ID :	02-0466515

Links to Exploration step

Pascal:02-0466515

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions</title>
<author><name sortKey="Lacey, J A" sort="Lacey, J A" uniqKey="Lacey J" first="J. A." last="Lacey">J. A. Lacey</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1>
<s2>Pocatello, ID 83209-8007</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Rodnick, K J" sort="Rodnick, K J" uniqKey="Rodnick K" first="K. J." last="Rodnick">K. J. Rodnick</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1>
<s2>Pocatello, ID 83209-8007</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">02-0466515</idno>
<date when="2002">2002</date>
<idno type="stanalyst">PASCAL 02-0466515 INIST</idno>
<idno type="RBID">Pascal:02-0466515</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000313</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions</title>
<author><name sortKey="Lacey, J A" sort="Lacey, J A" uniqKey="Lacey J" first="J. A." last="Lacey">J. A. Lacey</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1>
<s2>Pocatello, ID 83209-8007</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Rodnick, K J" sort="Rodnick, K J" uniqKey="Rodnick K" first="K. J." last="Rodnick">K. J. Rodnick</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1>
<s2>Pocatello, ID 83209-8007</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of fish biology</title>
<title level="j" type="abbreviated">J. fish biol.</title>
<idno type="ISSN">0022-1112</idno>
<imprint><date when="2002">2002</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of fish biology</title>
<title level="j" type="abbreviated">J. fish biol.</title>
<idno type="ISSN">0022-1112</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term>
<term>Blood</term>
<term>Ictalurus punctatus</term>
<term>Interspecific comparison</term>
<term>Measurement method</term>
<term>Methemoglobin</term>
<term>Oncorhynchus mykiss</term>
<term>Spectrophotometry</term>
<term>Tilapia nilotica</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Sang</term>
<term>Méthémoglobine</term>
<term>Spectrophotométrie</term>
<term>Méthode mesure</term>
<term>Comparaison interspécifique</term>
<term>Oncorhynchus mykiss</term>
<term>Ictalurus punctatus</term>
<term>Tilapia nilotica</term>
<term>Acipenser transmontanus</term>
<term>Tilapia zillii</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-1112</s0>
</fA01>
<fA02 i1="01"><s0>JFIBA9</s0>
</fA02>
<fA03 i2="1"><s0>J. fish biol.</s0>
</fA03>
<fA05><s2>60</s2>
</fA05>
<fA06><s2>5</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LACEY (J. A.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>RODNICK (K. J.)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Biological Sciences, Idaho State University</s1>
<s2>Pocatello, ID 83209-8007</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</fA14>
<fA20><s1>1155-1169</s1>
</fA20>
<fA21><s1>2002</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>14279</s2>
<s5>354000104406760080</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2002 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>1 p.1/4</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>02-0466515</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of fish biology</s0>
</fA64>
<fA66 i1="01"><s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A21</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Sang</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Blood</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Sangre</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Méthémoglobine</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Methemoglobin</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Metahemoglobina</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Spectrophotométrie</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Spectrophotometry</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Espectrofotometría</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Méthode mesure</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Measurement method</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Método medida</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Comparaison interspécifique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Interspecific comparison</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Comparación interespecífica</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Oncorhynchus mykiss</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Oncorhynchus mykiss</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Oncorhynchus mykiss</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Ictalurus punctatus</s0>
<s2>NS</s2>
<s5>56</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Ictalurus punctatus</s0>
<s2>NS</s2>
<s5>56</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Ictalurus punctatus</s0>
<s2>NS</s2>
<s5>56</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Tilapia nilotica</s0>
<s2>NS</s2>
<s5>57</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Tilapia nilotica</s0>
<s2>NS</s2>
<s5>57</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Tilapia nilotica</s0>
<s2>NS</s2>
<s5>57</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Acipenser transmontanus</s0>
<s2>NS</s2>
<s5>58</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Acipenser transmontanus</s0>
<s2>NS</s2>
<s5>58</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Acipenser transmontanus</s0>
<s2>NS</s2>
<s5>58</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Tilapia zillii</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>65</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Milieu aquatique</s0>
<s5>17</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Aquatic environment</s0>
<s5>17</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Medio acuático</s0>
<s5>17</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Cichlidae</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Cichlidae</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Cichlidae</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Pisces</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Pisces</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Pisces</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Salmonidae</s0>
<s2>NS</s2>
<s5>60</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Salmonidae</s0>
<s2>NS</s2>
<s5>60</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Salmonidae</s0>
<s2>NS</s2>
<s5>60</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Ictaluridae</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>71</s5>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Acipenseridae</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>73</s5>
</fC07>
<fN21><s1>273</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 02-0466515 INIST</NO>
<ET>Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions</ET>
<AU>LACEY (J. A.); RODNICK (K. J.)</AU>
<AF>Department of Biological Sciences, Idaho State University/Pocatello, ID 83209-8007/Etats-Unis (1 aut., 2 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of fish biology; ISSN 0022-1112; Coden JFIBA9; Royaume-Uni; Da. 2002; Vol. 60; No. 5; Pp. 1155-1169; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>Spectrophotometric assays of methaemoglobin (metHb) in rainbow trout Oncorhynchus mykiss, channel catfish Ictalurus punctatus, tilapias Tilapia niloticus and Tilapia zillii and white sturgeon Acipenser transmontanus, under baseline conditions, were low (<4%) for each species, and yet higher than human values (<1%). MetHb results for a given fish species varied significantly between assays and two assays were deemed unacceptable for particular animals. For rainbow trout, white sturgeon, and the two species of tilapia, the Dubowski method gave uncharacteristically high estimates of metHb. MetHb could not measured in tilapia blood using the Evelyn & Malloy method due to spectral interference. Only the Horecker & Brackett assay worked well for all species. Storage conditions were extremely important in the quantification of metHb in rainbow trout blood. For consistent values, samples can be stored up to 4 h on ice (0° C) or at least 20 days under liquid nitrogen ( - 196° C). Auto-oxidation, however, elevates rainbow trout metHb at - 20 and - 80° C. It should not be assumed that the blood of fishes and humans perform similarly during assays of metHb.</EA>
<CC>002A21</CC>
<FD>Sang; Méthémoglobine; Spectrophotométrie; Méthode mesure; Comparaison interspécifique; Oncorhynchus mykiss; Ictalurus punctatus; Tilapia nilotica; Acipenser transmontanus; Tilapia zillii</FD>
<FG>Milieu aquatique; Cichlidae; Pisces; Vertebrata; Salmonidae; Ictaluridae; Acipenseridae</FG>
<ED>Blood; Methemoglobin; Spectrophotometry; Measurement method; Interspecific comparison; Oncorhynchus mykiss; Ictalurus punctatus; Tilapia nilotica; Acipenser transmontanus</ED>
<EG>Aquatic environment; Cichlidae; Pisces; Vertebrata; Salmonidae</EG>
<SD>Sangre; Metahemoglobina; Espectrofotometría; Método medida; Comparación interespecífica; Oncorhynchus mykiss; Ictalurus punctatus; Tilapia nilotica; Acipenser transmontanus</SD>
<LO>INIST-14279.354000104406760080</LO>
<ID>02-0466515</ID>
</server>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PascalFrancis/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000313 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000313 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Eau
   |area=    EsturgeonV1
   |flux=    PascalFrancis
   |étape=   Corpus
   |type=    RBID
   |clé=     Pascal:02-0466515
   |texte=   Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions
}}

This area was generated with Dilib version V0.6.27.
Data generation: Sat Mar 25 15:37:54 2017. Site generation: Tue Feb 13 14:18:49 2024

	Serveur d'exploration sur l'esturgeon
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'esturgeon

Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions

Important considerations for methaemoglobin measurement in fish blood: assay choice and storage conditions

Source :

Descripteurs français

English descriptors

Abstract

Notice en format standard (ISO 2709)

Format Inist (serveur)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri