Serveur d'exploration sur l'esturgeon

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: Molecular cloning and distribution of the mRNAs encoding two somatostatin precursors

Identifieur interne : 001392 ( Main/Merge ); précédent : 001391; suivant : 001393

Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: Molecular cloning and distribution of the mRNAs encoding two somatostatin precursors

Auteurs : Michele Trabucchi [France, Italie] ; Hervé Tostivint [France] ; Isabelle Lihrmann [France] ; Cristina Sollars [États-Unis] ; Mauro Vallarino [Italie] ; Robert M. Dores [États-Unis] ; Hubert Vaudry [France]

Source :

RBID : ISTEX:2B4B9D73DE9A34FEE01ED9FB22541906AE9EB076

English descriptors

Abstract

The sequence of somatostatin‐14 (SS1) has been strongly preserved throughout the evolution of vertebrates from agnathans to mammals. In Acipenseridae (sturgeons), two isoforms of somatostatin have been characterized to date: somatostatin‐14 has been identified from the gastrointestinal tract of the pallid sturgeon Scaphirhynchus albus and [Pro2]somatostatin‐14 has been identified from the pituitary of the Russian sturgeon Acipenser gueldenstaedti. In the present study, we report the cloning of two distinct somatostatin cDNAs from the brain of the sturgeon Acipenser transmontanus. One of the cDNAs encodes a 116‐amino acid protein (PSS1) that contains the SS1 sequence at its C‐terminal extremity and, thus, is clearly orthologous to other vertebrate PSS1. The other cDNA encodes a 111‐amino acid protein that contains the somatostatin variant [Pro2]somatostatin‐14 at its C‐terminal extremity. This second precursor exhibits more than 67% identity with the recently characterized lungfish PSS2 and goldfish PSS2. Reverse transcriptase‐polymerase chain reaction analysis revealed that PSS1 is expressed in the central nervous system, the pancreas and the gut, whereas PSS2 is found in the central nervous system but not in the digestive system. In situ hybridization histochemistry showed that the PSS1 and PSS2 genes are differently expressed in numerous regions of the sturgeon brain. Interestingly, PSS1 and PSS2 mRNAs are present in the hypothalamus suggesting that, in sturgeon, both SS1 and SS2 may play hypophysiotropic functions. The PSS2 mRNA but not the PSS1 mRNA was found in the intermediate lobe of the pituitary. The present data demonstrate that two somatostatin genes are expressed in the sturgeon brain: one precursor generates somatostatin‐14 and the other one gives rise to a [Pro2]somatostatin‐14 variant, which is orthologous to goldfish, lungfish, and frog SS2. J. Comp. Neurol. 443:332–345, 2002. © 2002 Wiley‐Liss, Inc.

Url:
DOI: 10.1002/cne.10126

Links toward previous steps (curation, corpus...)


Links to Exploration step

ISTEX:2B4B9D73DE9A34FEE01ED9FB22541906AE9EB076

Le document en format XML

<record>
<TEI wicri:istexFullTextTei="biblStruct">
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: Molecular cloning and distribution of the mRNAs encoding two somatostatin precursors</title>
<author>
<name sortKey="Trabucchi, Michele" sort="Trabucchi, Michele" uniqKey="Trabucchi M" first="Michele" last="Trabucchi">Michele Trabucchi</name>
</author>
<author>
<name sortKey="Tostivint, Herve" sort="Tostivint, Herve" uniqKey="Tostivint H" first="Hervé" last="Tostivint">Hervé Tostivint</name>
</author>
<author>
<name sortKey="Lihrmann, Isabelle" sort="Lihrmann, Isabelle" uniqKey="Lihrmann I" first="Isabelle" last="Lihrmann">Isabelle Lihrmann</name>
</author>
<author>
<name sortKey="Sollars, Cristina" sort="Sollars, Cristina" uniqKey="Sollars C" first="Cristina" last="Sollars">Cristina Sollars</name>
</author>
<author>
<name sortKey="Vallarino, Mauro" sort="Vallarino, Mauro" uniqKey="Vallarino M" first="Mauro" last="Vallarino">Mauro Vallarino</name>
</author>
<author>
<name sortKey="Dores, Robert M" sort="Dores, Robert M" uniqKey="Dores R" first="Robert M." last="Dores">Robert M. Dores</name>
</author>
<author>
<name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:2B4B9D73DE9A34FEE01ED9FB22541906AE9EB076</idno>
<date when="2002" year="2002">2002</date>
<idno type="doi">10.1002/cne.10126</idno>
<idno type="url">https://api.istex.fr/document/2B4B9D73DE9A34FEE01ED9FB22541906AE9EB076/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001344</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001344</idno>
<idno type="wicri:Area/Istex/Curation">001342</idno>
<idno type="wicri:Area/Istex/Checkpoint">000B20</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B20</idno>
<idno type="wicri:doubleKey">0021-9967:2002:Trabucchi M:polygenic:expression:of</idno>
<idno type="wicri:Area/Main/Merge">001392</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title level="a" type="main" xml:lang="en">Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: Molecular cloning and distribution of the mRNAs encoding two somatostatin precursors</title>
<author>
<name sortKey="Trabucchi, Michele" sort="Trabucchi, Michele" uniqKey="Trabucchi M" first="Michele" last="Trabucchi">Michele Trabucchi</name>
<affiliation wicri:level="3">
<country xml:lang="fr">France</country>
<wicri:regionArea>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Santé et de la Recherche Médicale (INSERM U‐413), Unité Affiliée au Centre National de la Recherche Scientifique (UA CNRS), University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea>
<placeName>
<region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Mont‐Saint‐Aignan</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1">
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Experimental Biology, DIBISAA, University of Genova, 16132 Genova</wicri:regionArea>
<wicri:noRegion>16132 Genova</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Tostivint, Herve" sort="Tostivint, Herve" uniqKey="Tostivint H" first="Hervé" last="Tostivint">Hervé Tostivint</name>
<affiliation wicri:level="3">
<country xml:lang="fr">France</country>
<wicri:regionArea>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Santé et de la Recherche Médicale (INSERM U‐413), Unité Affiliée au Centre National de la Recherche Scientifique (UA CNRS), University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea>
<placeName>
<region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Mont‐Saint‐Aignan</settlement>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Lihrmann, Isabelle" sort="Lihrmann, Isabelle" uniqKey="Lihrmann I" first="Isabelle" last="Lihrmann">Isabelle Lihrmann</name>
<affiliation wicri:level="3">
<country xml:lang="fr">France</country>
<wicri:regionArea>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Santé et de la Recherche Médicale (INSERM U‐413), Unité Affiliée au Centre National de la Recherche Scientifique (UA CNRS), University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea>
<placeName>
<region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Mont‐Saint‐Aignan</settlement>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Sollars, Cristina" sort="Sollars, Cristina" uniqKey="Sollars C" first="Cristina" last="Sollars">Cristina Sollars</name>
<affiliation wicri:level="2">
<country xml:lang="fr">États-Unis</country>
<placeName>
<region type="state">Colorado</region>
</placeName>
<wicri:cityArea>Department of Biological Sciences, University of Denver, Denver</wicri:cityArea>
</affiliation>
</author>
<author>
<name sortKey="Vallarino, Mauro" sort="Vallarino, Mauro" uniqKey="Vallarino M" first="Mauro" last="Vallarino">Mauro Vallarino</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Experimental Biology, DIBISAA, University of Genova, 16132 Genova</wicri:regionArea>
<wicri:noRegion>16132 Genova</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Dores, Robert M" sort="Dores, Robert M" uniqKey="Dores R" first="Robert M." last="Dores">Robert M. Dores</name>
<affiliation wicri:level="2">
<country xml:lang="fr">États-Unis</country>
<placeName>
<region type="state">Colorado</region>
</placeName>
<wicri:cityArea>Department of Biological Sciences, University of Denver, Denver</wicri:cityArea>
</affiliation>
</author>
<author>
<name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name>
<affiliation wicri:level="3">
<country xml:lang="fr">France</country>
<wicri:regionArea>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Santé et de la Recherche Médicale (INSERM U‐413), Unité Affiliée au Centre National de la Recherche Scientifique (UA CNRS), University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea>
<placeName>
<region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Mont‐Saint‐Aignan</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3">
<country xml:lang="fr">France</country>
<wicri:regionArea>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U‐413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea>
<placeName>
<region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Mont‐Saint‐Aignan</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series>
<title level="j">Journal of Comparative Neurology</title>
<title level="j" type="abbrev">J. Comp. Neurol.</title>
<idno type="ISSN">0021-9967</idno>
<idno type="eISSN">1096-9861</idno>
<imprint>
<publisher>John Wiley & Sons, Inc.</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="2002-02-18">2002-02-18</date>
<biblScope unit="volume">443</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="332">332</biblScope>
<biblScope unit="page" to="345">345</biblScope>
</imprint>
<idno type="ISSN">0021-9967</idno>
</series>
<idno type="istex">2B4B9D73DE9A34FEE01ED9FB22541906AE9EB076</idno>
<idno type="DOI">10.1002/cne.10126</idno>
<idno type="ArticleID">CNE10126</idno>
</biblStruct>
</sourceDesc>
<seriesStmt>
<idno type="ISSN">0021-9967</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>SRIF</term>
<term>central nervous system</term>
<term>cortistatin</term>
<term>evolution</term>
<term>ray‐finned fish</term>
</keywords>
</textClass>
<langUsage>
<language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">The sequence of somatostatin‐14 (SS1) has been strongly preserved throughout the evolution of vertebrates from agnathans to mammals. In Acipenseridae (sturgeons), two isoforms of somatostatin have been characterized to date: somatostatin‐14 has been identified from the gastrointestinal tract of the pallid sturgeon Scaphirhynchus albus and [Pro2]somatostatin‐14 has been identified from the pituitary of the Russian sturgeon Acipenser gueldenstaedti. In the present study, we report the cloning of two distinct somatostatin cDNAs from the brain of the sturgeon Acipenser transmontanus. One of the cDNAs encodes a 116‐amino acid protein (PSS1) that contains the SS1 sequence at its C‐terminal extremity and, thus, is clearly orthologous to other vertebrate PSS1. The other cDNA encodes a 111‐amino acid protein that contains the somatostatin variant [Pro2]somatostatin‐14 at its C‐terminal extremity. This second precursor exhibits more than 67% identity with the recently characterized lungfish PSS2 and goldfish PSS2. Reverse transcriptase‐polymerase chain reaction analysis revealed that PSS1 is expressed in the central nervous system, the pancreas and the gut, whereas PSS2 is found in the central nervous system but not in the digestive system. In situ hybridization histochemistry showed that the PSS1 and PSS2 genes are differently expressed in numerous regions of the sturgeon brain. Interestingly, PSS1 and PSS2 mRNAs are present in the hypothalamus suggesting that, in sturgeon, both SS1 and SS2 may play hypophysiotropic functions. The PSS2 mRNA but not the PSS1 mRNA was found in the intermediate lobe of the pituitary. The present data demonstrate that two somatostatin genes are expressed in the sturgeon brain: one precursor generates somatostatin‐14 and the other one gives rise to a [Pro2]somatostatin‐14 variant, which is orthologous to goldfish, lungfish, and frog SS2. J. Comp. Neurol. 443:332–345, 2002. © 2002 Wiley‐Liss, Inc.</div>
</front>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001392 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001392 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Eau
   |area=    EsturgeonV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     ISTEX:2B4B9D73DE9A34FEE01ED9FB22541906AE9EB076
   |texte=   Polygenic expression of somatostatin in the sturgeon Acipenser transmontanus: Molecular cloning and distribution of the mRNAs encoding two somatostatin precursors
}}

Wicri

This area was generated with Dilib version V0.6.27.
Data generation: Sat Mar 25 15:37:54 2017. Site generation: Tue Feb 13 14:18:49 2024