Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system
Identifieur interne : 001338 ( Main/Merge ); précédent : 001337; suivant : 001339Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system
Auteurs : L. Kuznetsova [Russie] ; A. Shpakov [Russie] ; Yu. Rusakov [Russie] ; S. Plesneva [Russie] ; V. Bondareva [Russie] ; M. Pertseva [Russie]Source :
- Regulatory peptides [ 0167-0115 ] ; 2003.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC50, i.e. concentration leading to half-maximal activating effect of the hormone (10-11-10-7 M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000266
- to stream PascalFrancis, to step Curation: 000110
- to stream PascalFrancis, to step Checkpoint: 000294
Links to Exploration step
Pascal:04-0497360Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system</title>
<author><name sortKey="Kuznetsova, L" sort="Kuznetsova, L" uniqKey="Kuznetsova L" first="L." last="Kuznetsova">L. Kuznetsova</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Shpakov, A" sort="Shpakov, A" uniqKey="Shpakov A" first="A." last="Shpakov">A. Shpakov</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu." last="Rusakov">Yu. Rusakov</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S." last="Plesneva">S. Plesneva</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V." last="Bondareva">V. Bondareva</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M." last="Pertseva">M. Pertseva</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">04-0497360</idno>
<date when="2003">2003</date>
<idno type="stanalyst">PASCAL 04-0497360 INIST</idno>
<idno type="RBID">Pascal:04-0497360</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000266</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000110</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000294</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000294</idno>
<idno type="wicri:doubleKey">0167-0115:2003:Kuznetsova L:comparative:study:of</idno>
<idno type="wicri:Area/Main/Merge">001338</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system</title>
<author><name sortKey="Kuznetsova, L" sort="Kuznetsova, L" uniqKey="Kuznetsova L" first="L." last="Kuznetsova">L. Kuznetsova</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Shpakov, A" sort="Shpakov, A" uniqKey="Shpakov A" first="A." last="Shpakov">A. Shpakov</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu." last="Rusakov">Yu. Rusakov</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S." last="Plesneva">S. Plesneva</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V." last="Bondareva">V. Bondareva</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M." last="Pertseva">M. Pertseva</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1>
<s2>194223 St. Petersburg</s2>
<s3>RUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Russie</country>
<wicri:noRegion>194223 St. Petersburg</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Regulatory peptides</title>
<title level="j" type="abbreviated">Regul. pept.</title>
<idno type="ISSN">0167-0115</idno>
<imprint><date when="2003">2003</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Regulatory peptides</title>
<title level="j" type="abbreviated">Regul. pept.</title>
<idno type="ISSN">0167-0115</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenylate cyclase</term>
<term>Biological activity</term>
<term>Comparative study</term>
<term>Insulin</term>
<term>Peptides</term>
<term>Signal transduction</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Etude comparative</term>
<term>Activité biologique</term>
<term>Insuline</term>
<term>Adenylate cyclase</term>
<term>Peptide</term>
<term>Transduction signal</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC<sub>50</sub>
, i.e. concentration leading to half-maximal activating effect of the hormone (10<sup>-11</sup>
-10<sup>-7</sup>
M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.</div>
</front>
</TEI>
<affiliations><list><country><li>Russie</li>
</country>
</list>
<tree><country name="Russie"><noRegion><name sortKey="Kuznetsova, L" sort="Kuznetsova, L" uniqKey="Kuznetsova L" first="L." last="Kuznetsova">L. Kuznetsova</name>
</noRegion>
<name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V." last="Bondareva">V. Bondareva</name>
<name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M." last="Pertseva">M. Pertseva</name>
<name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S." last="Plesneva">S. Plesneva</name>
<name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu." last="Rusakov">Yu. Rusakov</name>
<name sortKey="Shpakov, A" sort="Shpakov, A" uniqKey="Shpakov A" first="A." last="Shpakov">A. Shpakov</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001338 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001338 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Merge |type= RBID |clé= Pascal:04-0497360 |texte= Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |