Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system
Identifieur interne : 000294 ( PascalFrancis/Checkpoint ); précédent : 000293; suivant : 000295Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system
Auteurs : L. Kuznetsova [Russie] ; A. Shpakov [Russie] ; Yu. Rusakov [Russie] ; S. Plesneva [Russie] ; V. Bondareva [Russie] ; M. Pertseva [Russie]Source :
- Regulatory peptides [ 0167-0115 ] ; 2003.
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Abstract
The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC50, i.e. concentration leading to half-maximal activating effect of the hormone (10-11-10-7 M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.
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Shpakov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu." last="Rusakov">Yu. Rusakov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S." last="Plesneva">S. Plesneva</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V." last="Bondareva">V. Bondareva</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M." last="Pertseva">M. Pertseva</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0497360</idno><date when="2003">2003</date><idno type="stanalyst">PASCAL 04-0497360 INIST</idno><idno type="RBID">Pascal:04-0497360</idno><idno type="wicri:Area/PascalFrancis/Corpus">000266</idno><idno type="wicri:Area/PascalFrancis/Curation">000110</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000294</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000294</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system</title><author><name sortKey="Kuznetsova, L" sort="Kuznetsova, L" uniqKey="Kuznetsova L" first="L." last="Kuznetsova">L. Kuznetsova</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Shpakov, A" sort="Shpakov, A" uniqKey="Shpakov A" first="A." last="Shpakov">A. Shpakov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu." last="Rusakov">Yu. Rusakov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S." last="Plesneva">S. Plesneva</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V." last="Bondareva">V. Bondareva</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M." last="Pertseva">M. Pertseva</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Russie</country><wicri:noRegion>194223 St. Petersburg</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Regulatory peptides</title><title level="j" type="abbreviated">Regul. pept.</title><idno type="ISSN">0167-0115</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Regulatory peptides</title><title level="j" type="abbreviated">Regul. pept.</title><idno type="ISSN">0167-0115</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenylate cyclase</term><term>Biological activity</term><term>Comparative study</term><term>Insulin</term><term>Peptides</term><term>Signal transduction</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Etude comparative</term><term>Activité biologique</term><term>Insuline</term><term>Adenylate cyclase</term><term>Peptide</term><term>Transduction signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC<sub>50</sub>, i.e. concentration leading to half-maximal activating effect of the hormone (10<sup>-11</sup>-10<sup>-7</sup> M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0167-0115</s0></fA01><fA02 i1="01"><s0>REPPDY</s0></fA02><fA03 i2="1"><s0>Regul. pept.</s0></fA03><fA05><s2>116</s2></fA05><fA06><s2>1-3</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system</s1></fA08><fA11 i1="01" i2="1"><s1>KUZNETSOVA (L.)</s1></fA11><fA11 i1="02" i2="1"><s1>SHPAKOV (A.)</s1></fA11><fA11 i1="03" i2="1"><s1>RUSAKOV (Yu.)</s1></fA11><fA11 i1="04" i2="1"><s1>PLESNEVA (S.)</s1></fA11><fA11 i1="05" i2="1"><s1>BONDAREVA (V.)</s1></fA11><fA11 i1="06" i2="1"><s1>PERTSEVA (M.)</s1></fA11><fA14 i1="01"><s1>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44</s1><s2>194223 St. Petersburg</s2><s3>RUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></fA14><fA20><s1>81-86</s1></fA20><fA21><s1>2003</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18854</s2><s5>354000112679530100</s5></fA43><fA44><s0>0000</s0><s1>© 2004 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>25 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>04-0497360</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Regulatory peptides</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC<sub>50</sub>, i.e. concentration leading to half-maximal activating effect of the hormone (10<sup>-11</sup>-10<sup>-7</sup> M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.</s0></fC01><fC02 i1="01" i2="X"><s0>002A28</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Etude comparative</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Comparative study</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Estudio comparativo</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Activité biologique</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Biological activity</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Actividad biológica</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Insuline</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Insulin</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Insulina</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Adenylate cyclase</s0><s2>FE</s2><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Adenylate cyclase</s0><s2>FE</s2><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Adenylate cyclase</s0><s2>FE</s2><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Peptide</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Peptides</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Péptido</s0><s5>07</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Transduction signal</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Signal transduction</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Transducción señal</s0><s5>08</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Phosphorus-oxygen lyases</s0><s2>FE</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Phosphorus-oxygen lyases</s0><s2>FE</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Phosphorus-oxygen lyases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Lyases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Lyases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Lyases</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Enzyme</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Enzyme</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Enzima</s0><s2>FE</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Hormone pancréatique</s0><s5>20</s5></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Pancreatic hormone</s0><s5>20</s5></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Hormona pancreática</s0><s5>20</s5></fC07><fN21><s1>285</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Russie</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Kuznetsova, L" sort="Kuznetsova, L" uniqKey="Kuznetsova L" first="L." last="Kuznetsova">L. Kuznetsova</name></noRegion><name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V." last="Bondareva">V. Bondareva</name><name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M." last="Pertseva">M. Pertseva</name><name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S." last="Plesneva">S. Plesneva</name><name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu." last="Rusakov">Yu. Rusakov</name><name sortKey="Shpakov, A" sort="Shpakov, A" uniqKey="Shpakov A" first="A." last="Shpakov">A. Shpakov</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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