EsturgeonV1, Main, Merge, bibRecord, 000D87

Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay

Identifieur interne : 000D87 ( Main/Merge ); précédent : 000D86; suivant : 000D88

Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay

Auteurs : Kevin T. Kwak [États-Unis] ; Ian A. Gardner [États-Unis] ; Thomas B. Farver [États-Unis] ; Ronald P. Hedrick [États-Unis]

Source :

Aquaculture : (Amsterdam) [ 0044-8486 ] ; 2006.

RBID : Pascal:06-0265987

Descripteurs français

Pascal (Inist)
- Détection, Réaction chaîne polymérase, Histologie, Capside, Protéine, Aquiculture, Acipenser transmontanus, Iridovirus.
Wicri :
- topic : Histologie, Aquiculture.

English descriptors

KwdEn :
- Acipenser transmontanus, Aquaculture, Capsid, Detection, Histology, Iridovirus, Polymerase chain reaction, Protein.

Abstract

A polymerase chain reaction (PCR) assay was developed for detection of white sturgeon iridovirus (WSIV). The synthetic oligonucleotides WS 229F and WS 245R generate a 519 bp DNA amplicon from the WSIV genome that encodes the putative major capsid protein (MCP) gene. The WSIV PCR detected as little as 1 fg of plasmid DNA as mixed with 100 ng of host DNA. No amplicons were detected with the WSIV PCR assay upon testing of genomic DNA from uninfected white sturgeon spleen cell line (WSS-2), uninfected white sturgeon tissues, white sturgeon adenovirus (WSAV), Acipenserid herpesvirus 2 (AcHV-2), frog virus 3 (FV-3), epizootic haematopoietic necrosis virus (EHNV), and red sea bream iridovirus (RSIV). The newly developed PCR assay was also evaluated with groups of juvenile white sturgeon following experimental exposures to WSIV. An analysis of WSIV detection by PCR compared to current histological methods provided sensitivity estimates of 98% and 64% for PCR and histology, respectively. This new PCR assay provides a more rapid and sensitive approach for the detection of WSIV infections among juvenile white sturgeon than currently available diagnostic approaches including virus isolation and microscopic examinations of stained tissue sections.

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000216
to stream PascalFrancis, to step Curation: 000160
to stream PascalFrancis, to step Checkpoint: 000197

Links to Exploration step

Pascal:06-0265987

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay</title>
<author><name sortKey="Kwak, Kevin T" sort="Kwak, Kevin T" uniqKey="Kwak K" first="Kevin T." last="Kwak">Kevin T. Kwak</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1>
<s2>Davis. CA 95616</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Davis. CA 95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gardner, Ian A" sort="Gardner, Ian A" uniqKey="Gardner I" first="Ian A." last="Gardner">Ian A. Gardner</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1>
<s2>Davis. CA 95616</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Davis. CA 95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Farver, Thomas B" sort="Farver, Thomas B" uniqKey="Farver T" first="Thomas B." last="Farver">Thomas B. Farver</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Population Health and Reproduction, School of Veterinary Medicine, University of California</s1>
<s2>Davis, CA 95616</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hedrick, Ronald P" sort="Hedrick, Ronald P" uniqKey="Hedrick R" first="Ronald P." last="Hedrick">Ronald P. Hedrick</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1>
<s2>Davis. CA 95616</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Davis. CA 95616</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">06-0265987</idno>
<date when="2006">2006</date>
<idno type="stanalyst">PASCAL 06-0265987 INIST</idno>
<idno type="RBID">Pascal:06-0265987</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000216</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000160</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000197</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000197</idno>
<idno type="wicri:doubleKey">0044-8486:2006:Kwak K:rapid:detection:of</idno>
<idno type="wicri:Area/Main/Merge">000D87</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay</title>
<author><name sortKey="Kwak, Kevin T" sort="Kwak, Kevin T" uniqKey="Kwak K" first="Kevin T." last="Kwak">Kevin T. Kwak</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1>
<s2>Davis. CA 95616</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Davis. CA 95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gardner, Ian A" sort="Gardner, Ian A" uniqKey="Gardner I" first="Ian A." last="Gardner">Ian A. Gardner</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1>
<s2>Davis. CA 95616</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Davis. CA 95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Farver, Thomas B" sort="Farver, Thomas B" uniqKey="Farver T" first="Thomas B." last="Farver">Thomas B. Farver</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Department of Population Health and Reproduction, School of Veterinary Medicine, University of California</s1>
<s2>Davis, CA 95616</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hedrick, Ronald P" sort="Hedrick, Ronald P" uniqKey="Hedrick R" first="Ronald P." last="Hedrick">Ronald P. Hedrick</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California</s1>
<s2>Davis. CA 95616</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Davis. CA 95616</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Aquaculture : (Amsterdam)</title>
<title level="j" type="abbreviated">Aquaculture : (Amst.)</title>
<idno type="ISSN">0044-8486</idno>
<imprint><date when="2006">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Aquaculture : (Amsterdam)</title>
<title level="j" type="abbreviated">Aquaculture : (Amst.)</title>
<idno type="ISSN">0044-8486</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser transmontanus</term>
<term>Aquaculture</term>
<term>Capsid</term>
<term>Detection</term>
<term>Histology</term>
<term>Iridovirus</term>
<term>Polymerase chain reaction</term>
<term>Protein</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Détection</term>
<term>Réaction chaîne polymérase</term>
<term>Histologie</term>
<term>Capside</term>
<term>Protéine</term>
<term>Aquiculture</term>
<term>Acipenser transmontanus</term>
<term>Iridovirus</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Histologie</term>
<term>Aquiculture</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A polymerase chain reaction (PCR) assay was developed for detection of white sturgeon iridovirus (WSIV). The synthetic oligonucleotides WS 229F and WS 245R generate a 519 bp DNA amplicon from the WSIV genome that encodes the putative major capsid protein (MCP) gene. The WSIV PCR detected as little as 1 fg of plasmid DNA as mixed with 100 ng of host DNA. No amplicons were detected with the WSIV PCR assay upon testing of genomic DNA from uninfected white sturgeon spleen cell line (WSS-2), uninfected white sturgeon tissues, white sturgeon adenovirus (WSAV), Acipenserid herpesvirus 2 (AcHV-2), frog virus 3 (FV-3), epizootic haematopoietic necrosis virus (EHNV), and red sea bream iridovirus (RSIV). The newly developed PCR assay was also evaluated with groups of juvenile white sturgeon following experimental exposures to WSIV. An analysis of WSIV detection by PCR compared to current histological methods provided sensitivity estimates of 98% and 64% for PCR and histology, respectively. This new PCR assay provides a more rapid and sensitive approach for the detection of WSIV infections among juvenile white sturgeon than currently available diagnostic approaches including virus isolation and microscopic examinations of stained tissue sections.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Kwak, Kevin T" sort="Kwak, Kevin T" uniqKey="Kwak K" first="Kevin T." last="Kwak">Kevin T. Kwak</name>
</noRegion>
<name sortKey="Farver, Thomas B" sort="Farver, Thomas B" uniqKey="Farver T" first="Thomas B." last="Farver">Thomas B. Farver</name>
<name sortKey="Gardner, Ian A" sort="Gardner, Ian A" uniqKey="Gardner I" first="Ian A." last="Gardner">Ian A. Gardner</name>
<name sortKey="Hedrick, Ronald P" sort="Hedrick, Ronald P" uniqKey="Hedrick R" first="Ronald P." last="Hedrick">Ronald P. Hedrick</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D87 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000D87 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Eau
   |area=    EsturgeonV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     Pascal:06-0265987
   |texte=   Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay
}}

This area was generated with Dilib version V0.6.27.
Data generation: Sat Mar 25 15:37:54 2017. Site generation: Tue Feb 13 14:18:49 2024

	Serveur d'exploration sur l'esturgeon
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'esturgeon

Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay

Rapid detection of white sturgeon iridovirus (WSIV) using a polymerase chain reaction (PCR) assay

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri