Availability of in vitro vitellogenin assay for screening of estrogenic and anti-estrogenic activities of environmental chemicals.
Identifieur interne : 000C93 ( Main/Merge ); précédent : 000C92; suivant : 000C94Availability of in vitro vitellogenin assay for screening of estrogenic and anti-estrogenic activities of environmental chemicals.
Auteurs : Taisen Iguchi [Japon] ; Fumi Irie ; Hiroshi Urushitani ; Osamu Tooi ; Yukio Kawashima ; Mike Roberts ; Leif Norrgren ; Thomas HutchinsonSource :
- Environmental sciences : an international journal of environmental physiology and toxicology [ 0915-955X ] ; 2006.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Charadriiformes, Chickens, Coturnix, Environmental Pollutants (analysis), Enzyme-Linked Immunosorbent Assay (methods), Estrogens (analysis), Fishes, Hepatocytes (drug effects), Hepatocytes (metabolism), RNA, Messenger (biosynthesis), RNA, Messenger (genetics), Vitellogenins (analysis), Vitellogenins (biosynthesis), Vitellogenins (genetics), Xenopus laevis.
- MESH :
- chemical , analysis : Environmental Pollutants, Estrogens, Vitellogenins.
- chemical , biosynthesis : RNA, Messenger, Vitellogenins.
- drug effects : Hepatocytes.
- chemical , genetics : RNA, Messenger, Vitellogenins.
- metabolism : Hepatocytes.
- methods : Enzyme-Linked Immunosorbent Assay.
- Animals, Cells, Cultured, Charadriiformes, Chickens, Coturnix, Fishes, Xenopus laevis.
Abstract
Vitellogenin (VTG) protein, VTG mRNA, other egg yolk proteins, vitelline envelope proteins and their mRNAs are produced in the liver of oviparous species by stimulation of endogenous estrogen and exogenous estrogenic chemicals. The VTG assay based on enzyme-linked immunosorbent assay (ELISA) has been widely used for many fish species to screen estrogenic and anti-estrogenic activities of chemicals and sewage effluents using immature fish and/or male fish. In order to reduce the number of fish for screening of estrogenicity and anti-estrogenicity of chemicals, primary cultured fish hepatocytes can be used. In fact, primary cultured hepatocytes have been successfully used for the detection of estrogenic and anti-estrogenic activities of environmental chemicals in selected OECD fish species, e.g., medaka (Oryzias latipes) and rainbow trout (Oncorhynchys mykiss) together with other fish species such as Atlantic salmon (Salmo salar L.), Siberian sturgeon (Acipenser baeri), tilapia (Oreochromis mossambicus), carp (Cyprinus carpio), bream (Abramis brama), Carassius auratus, silver eel (Anguilla anguilla L.), and channel catfish (Ictalurus punctanus). In terms of hepatocyte assays relating to other taxa, these include frogs such as Xenopus laevis and the common green frog (Rana esculenta), chickens (Gallus domesticus) and herring gulls (Larus argentatus). VTG mRNA measurement by quantitative reverse transcription-polymerase chain reaction has also been successfully applied in the primary cultured hepatocytes of various species.
PubMed: 16883298
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last="Irie">Fumi Irie</name></author><author><name sortKey="Urushitani, Hiroshi" sort="Urushitani, Hiroshi" uniqKey="Urushitani H" first="Hiroshi" last="Urushitani">Hiroshi Urushitani</name></author><author><name sortKey="Tooi, Osamu" sort="Tooi, Osamu" uniqKey="Tooi O" first="Osamu" last="Tooi">Osamu Tooi</name></author><author><name sortKey="Kawashima, Yukio" sort="Kawashima, Yukio" uniqKey="Kawashima Y" first="Yukio" last="Kawashima">Yukio Kawashima</name></author><author><name sortKey="Roberts, Mike" sort="Roberts, Mike" uniqKey="Roberts M" first="Mike" last="Roberts">Mike Roberts</name></author><author><name sortKey="Norrgren, Leif" sort="Norrgren, Leif" uniqKey="Norrgren L" first="Leif" last="Norrgren">Leif Norrgren</name></author><author><name sortKey="Hutchinson, Thomas" sort="Hutchinson, Thomas" uniqKey="Hutchinson T" first="Thomas" last="Hutchinson">Thomas Hutchinson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date 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environmental chemicals.</title><author><name sortKey="Iguchi, Taisen" sort="Iguchi, Taisen" uniqKey="Iguchi T" first="Taisen" last="Iguchi">Taisen Iguchi</name><affiliation wicri:level="1"><nlm:affiliation>National Institutes of Natural Sciences, National Institute for Basic Biology, Okazaki Institute for Integrative Bioscience, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan. taisen@nibb.ac.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Institutes of Natural Sciences, National Institute for Basic Biology, Okazaki Institute for Integrative Bioscience, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787</wicri:regionArea><wicri:noRegion>Okazaki 444-8787</wicri:noRegion></affiliation></author><author><name sortKey="Irie, Fumi" sort="Irie, Fumi" uniqKey="Irie F" first="Fumi" last="Irie">Fumi Irie</name></author><author><name sortKey="Urushitani, Hiroshi" sort="Urushitani, Hiroshi" uniqKey="Urushitani H" first="Hiroshi" last="Urushitani">Hiroshi Urushitani</name></author><author><name sortKey="Tooi, Osamu" sort="Tooi, Osamu" uniqKey="Tooi O" first="Osamu" last="Tooi">Osamu Tooi</name></author><author><name sortKey="Kawashima, Yukio" sort="Kawashima, Yukio" uniqKey="Kawashima Y" first="Yukio" last="Kawashima">Yukio Kawashima</name></author><author><name sortKey="Roberts, Mike" sort="Roberts, Mike" uniqKey="Roberts M" first="Mike" last="Roberts">Mike Roberts</name></author><author><name sortKey="Norrgren, Leif" sort="Norrgren, Leif" uniqKey="Norrgren L" first="Leif" last="Norrgren">Leif Norrgren</name></author><author><name sortKey="Hutchinson, Thomas" sort="Hutchinson, Thomas" uniqKey="Hutchinson T" first="Thomas" last="Hutchinson">Thomas Hutchinson</name></author></analytic><series><title level="j">Environmental sciences : an international journal of environmental physiology and toxicology</title><idno type="ISSN">0915-955X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Charadriiformes</term><term>Chickens</term><term>Coturnix</term><term>Environmental Pollutants (analysis)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Estrogens (analysis)</term><term>Fishes</term><term>Hepatocytes (drug effects)</term><term>Hepatocytes (metabolism)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Messenger (genetics)</term><term>Vitellogenins (analysis)</term><term>Vitellogenins (biosynthesis)</term><term>Vitellogenins (genetics)</term><term>Xenopus laevis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Environmental Pollutants</term><term>Estrogens</term><term>Vitellogenins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Messenger</term><term>Vitellogenins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Hepatocytes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term><term>Vitellogenins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hepatocytes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Charadriiformes</term><term>Chickens</term><term>Coturnix</term><term>Fishes</term><term>Xenopus laevis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vitellogenin (VTG) protein, VTG mRNA, other egg yolk proteins, vitelline envelope proteins and their mRNAs are produced in the liver of oviparous species by stimulation of endogenous estrogen and exogenous estrogenic chemicals. The VTG assay based on enzyme-linked immunosorbent assay (ELISA) has been widely used for many fish species to screen estrogenic and anti-estrogenic activities of chemicals and sewage effluents using immature fish and/or male fish. In order to reduce the number of fish for screening of estrogenicity and anti-estrogenicity of chemicals, primary cultured fish hepatocytes can be used. In fact, primary cultured hepatocytes have been successfully used for the detection of estrogenic and anti-estrogenic activities of environmental chemicals in selected OECD fish species, e.g., medaka (Oryzias latipes) and rainbow trout (Oncorhynchys mykiss) together with other fish species such as Atlantic salmon (Salmo salar L.), Siberian sturgeon (Acipenser baeri), tilapia (Oreochromis mossambicus), carp (Cyprinus carpio), bream (Abramis brama), Carassius auratus, silver eel (Anguilla anguilla L.), and channel catfish (Ictalurus punctanus). In terms of hepatocyte assays relating to other taxa, these include frogs such as Xenopus laevis and the common green frog (Rana esculenta), chickens (Gallus domesticus) and herring gulls (Larus argentatus). VTG mRNA measurement by quantitative reverse transcription-polymerase chain reaction has also been successfully applied in the primary cultured hepatocytes of various species.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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