Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches
Identifieur interne : 000B94 ( Main/Merge ); précédent : 000B93; suivant : 000B95Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches
Auteurs : Maria Sen Mun Wai [République populaire de Chine] ; Dietrich Ernst Lorke [Émirats arabes unis, Allemagne] ; Aiqun Zhang [République populaire de Chine] ; Hsiang-Fu Kung [République populaire de Chine] ; David T. Yew [République populaire de Chine]Source :
- Microscopy Research and Technique [ 1059-910X ] ; 2007-12.
English descriptors
- KwdEn :
- Animals, Central Nervous System (cytology), Central Nervous System (enzymology), Goldfish (anatomy & histology), Goldfish (physiology), Immunohistochemistry, Microscopy, Electron, Scanning, Spinal Cord (anatomy & histology), Spinal Nerve Roots (anatomy & histology), fish, neurotransmitter, protein electrophoresis.
- MESH :
- anatomy & histology : Goldfish, Spinal Cord, Spinal Nerve Roots.
- cytology : Central Nervous System.
- enzymology : Central Nervous System.
- physiology : Goldfish.
- Animals, Immunohistochemistry, Microscopy, Electron, Scanning.
Abstract
Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jemt.20515
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Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.</div></front></TEI><double doi="10.1002/jemt.20515"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches</title><author><name sortKey="Wai, Maria Sen Mun" sort="Wai, Maria Sen Mun" uniqKey="Wai M" first="Maria Sen Mun" last="Wai">Maria Sen Mun Wai</name></author><author><name sortKey="Lorke, Dietrich Ernst" sort="Lorke, Dietrich Ernst" uniqKey="Lorke D" first="Dietrich Ernst" last="Lorke">Dietrich Ernst Lorke</name></author><author><name sortKey="Zhang, Aiqun" sort="Zhang, Aiqun" uniqKey="Zhang A" first="Aiqun" last="Zhang">Aiqun Zhang</name></author><author><name sortKey="Kung, Hsiang U" sort="Kung, Hsiang U" uniqKey="Kung H" first="Hsiang-Fu" last="Kung">Hsiang-Fu Kung</name></author><author><name sortKey="Yew, David T" sort="Yew, David T" uniqKey="Yew D" first="David T." last="Yew">David T. 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Yew</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anatomy, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Microscopy Research and Technique</title><title level="j" type="abbrev">Microsc. Res. Tech.</title><idno type="ISSN">1059-910X</idno><idno type="eISSN">1097-0029</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2007-12">2007-12</date><biblScope unit="volume">70</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1079">1079</biblScope><biblScope unit="page" to="1090">1090</biblScope></imprint><idno type="ISSN">1059-910X</idno></series><idno type="istex">71220740ECA44AFBEB3567BD3ACC5EF1901B9673</idno><idno type="DOI">10.1002/jemt.20515</idno><idno type="ArticleID">JEMT20515</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1059-910X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>fish</term><term>neurotransmitter</term><term>protein electrophoresis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.</div></front></TEI></ISTEX><PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches.</title><author><name sortKey="Wai, Maria Sen Mun" sort="Wai, Maria Sen Mun" uniqKey="Wai M" first="Maria Sen Mun" last="Wai">Maria Sen Mun Wai</name><affiliation wicri:level="4"><nlm:affiliation>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Lorke, Dietrich Ernst" sort="Lorke, Dietrich Ernst" uniqKey="Lorke D" first="Dietrich Ernst" last="Lorke">Dietrich Ernst Lorke</name></author><author><name sortKey="Zhang, Aiqun" sort="Zhang, Aiqun" uniqKey="Zhang A" first="Aiqun" last="Zhang">Aiqun Zhang</name></author><author><name sortKey="Kung, Hsiang Fu" sort="Kung, Hsiang Fu" uniqKey="Kung H" first="Hsiang-Fu" last="Kung">Hsiang-Fu Kung</name></author><author><name sortKey="Yew, David T" sort="Yew, David T" uniqKey="Yew D" first="David T" last="Yew">David T. Yew</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17868134</idno><idno type="pmid">17868134</idno><idno type="doi">10.1002/jemt.20515</idno><idno type="wicri:Area/PubMed/Corpus">000480</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000480</idno><idno type="wicri:Area/PubMed/Curation">000480</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000480</idno><idno type="wicri:Area/PubMed/Checkpoint">000480</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000480</idno><idno type="wicri:Area/Ncbi/Merge">000233</idno><idno type="wicri:Area/Ncbi/Curation">000233</idno><idno type="wicri:Area/Ncbi/Checkpoint">000233</idno><idno type="wicri:doubleKey">1059-910X:2007:Wai M:study:of:the</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches.</title><author><name sortKey="Wai, Maria Sen Mun" sort="Wai, Maria Sen Mun" uniqKey="Wai M" first="Maria Sen Mun" last="Wai">Maria Sen Mun Wai</name><affiliation wicri:level="4"><nlm:affiliation>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Lorke, Dietrich Ernst" sort="Lorke, Dietrich Ernst" uniqKey="Lorke D" first="Dietrich Ernst" last="Lorke">Dietrich Ernst Lorke</name></author><author><name sortKey="Zhang, Aiqun" sort="Zhang, Aiqun" uniqKey="Zhang A" first="Aiqun" last="Zhang">Aiqun Zhang</name></author><author><name sortKey="Kung, Hsiang Fu" sort="Kung, Hsiang Fu" uniqKey="Kung H" first="Hsiang-Fu" last="Kung">Hsiang-Fu Kung</name></author><author><name sortKey="Yew, David T" sort="Yew, David T" uniqKey="Yew D" first="David T" last="Yew">David T. Yew</name></author></analytic><series><title level="j">Microscopy research and technique</title><idno type="ISSN">1059-910X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Central Nervous System (cytology)</term><term>Central Nervous System (enzymology)</term><term>Goldfish (anatomy & histology)</term><term>Goldfish (physiology)</term><term>Immunohistochemistry</term><term>Microscopy, Electron, Scanning</term><term>Spinal Cord (anatomy & histology)</term><term>Spinal Nerve Roots (anatomy & histology)</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Goldfish</term><term>Spinal Cord</term><term>Spinal Nerve Roots</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Central Nervous System</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Central Nervous System</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Goldfish</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Immunohistochemistry</term><term>Microscopy, Electron, Scanning</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two-dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y-shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. gamma-aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5-HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5-HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two-dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord.</div></front></TEI></PubMed></double></record>Pour manipuler ce document sous Unix (Dilib)
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