The RAPD technique failed to identify sex‐specific sequences in beluga (Huso huso)
Identifieur interne : 000B91 ( Main/Merge ); précédent : 000B90; suivant : 000B92The RAPD technique failed to identify sex‐specific sequences in beluga (Huso huso)
Auteurs : S. Keyvanshokooh [Iran] ; M. Pourkazemi [Iran] ; M. R. Kalbassi [Iran]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2007-02.
Abstract
Because the sex of sturgeon cannot be determined externally until the pre‐spawning phase, they are currently sexed by means of minor surgery. Although the survival rate is nearly 100%, this surgery is invasive. One effective solution is to use DNA markers to diagnose the sex. In this study we employed bulked segregant analysis methodology to screen for genetic markers associated with the sex of the beluga (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 310 randomly amplified polymorphic DNA primers was used to screen the bulks, resulting in 4146 bands that were present in both sexes. Our results suggest that either the sex‐specific DNA in sturgeon may be comprised of sequences not complementary to 310 random decamer primers or that sex chromosomes are weakly differentiated in the sturgeon genome.
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DOI: 10.1111/j.1439-0426.2006.00798.x
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