The RAPD technique failed to identify sex‐specific sequences in beluga (Huso huso)
Identifieur interne : 000248 ( Istex/Corpus ); précédent : 000247; suivant : 000249The RAPD technique failed to identify sex‐specific sequences in beluga (Huso huso)
Auteurs : S. Keyvanshokooh ; M. Pourkazemi ; M. R. KalbassiSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2007-02.
Abstract
Because the sex of sturgeon cannot be determined externally until the pre‐spawning phase, they are currently sexed by means of minor surgery. Although the survival rate is nearly 100%, this surgery is invasive. One effective solution is to use DNA markers to diagnose the sex. In this study we employed bulked segregant analysis methodology to screen for genetic markers associated with the sex of the beluga (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 310 randomly amplified polymorphic DNA primers was used to screen the bulks, resulting in 4146 bands that were present in both sexes. Our results suggest that either the sex‐specific DNA in sturgeon may be comprised of sequences not complementary to 310 random decamer primers or that sex chromosomes are weakly differentiated in the sturgeon genome.
Url:
DOI: 10.1111/j.1439-0426.2006.00798.x
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Although the survival rate is nearly 100%, this surgery is invasive. One effective solution is to use DNA markers to diagnose the sex. In this study we employed bulked segregant analysis methodology to screen for genetic markers associated with the sex of the beluga (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 310 randomly amplified polymorphic DNA primers was used to screen the bulks, resulting in 4146 bands that were present in both sexes. Our results suggest that either the sex‐specific DNA in sturgeon may be comprised of sequences not complementary to 310 random decamer primers or that sex chromosomes are weakly differentiated in the sturgeon genome.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>S. 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E‐mail: <email>keyvan56@yahoo.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI798.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: February 20, 2006 Accepted: March 27, 2006</unparsedEditorialHistory><countGroup><count type="figureTotal" number="0"></count><count type="tableTotal" number="0"></count></countGroup><titleGroup><title type="main">The RAPD technique failed to identify sex‐specific sequences in beluga (<i>Huso huso</i>)</title><title type="shortAuthors">S. Keyvanshokooh, M. Pourkazemi and M. R. Kalbassi</title><title type="short">Identifying sex‐specific sequences in <i>H. huso</i></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>S.</givenNames><familyName>Keyvanshokooh</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>M.</givenNames><familyName>Pourkazemi</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>M. R.</givenNames><familyName>Kalbassi</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Department of Fisheries, College of Natural Resources and Marine Sciences, Tarbiat Modares University, Mazandaran, Iran</unparsedAffiliation></affiliation><affiliation xml:id="a2"><unparsedAffiliation>International Sturgeon Research Institute, Rasht, Iran</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>Because the sex of sturgeon cannot be determined externally until the pre‐spawning phase, they are currently sexed by means of minor surgery. Although the survival rate is nearly 100%, this surgery is invasive. One effective solution is to use DNA markers to diagnose the sex. In this study we employed bulked segregant analysis methodology to screen for genetic markers associated with the sex of the beluga (<i>Huso huso</i>). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 310 randomly amplified polymorphic DNA primers was used to screen the bulks, resulting in 4146 bands that were present in both sexes. Our results suggest that either the sex‐specific DNA in sturgeon may be comprised of sequences not complementary to 310 random decamer primers or that sex chromosomes are weakly differentiated in the sturgeon genome.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The RAPD technique failed to identify sex‐specific sequences in beluga (Huso huso)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Identifying sex‐specific sequences in H. huso</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>The RAPD technique failed to identify sex‐specific sequences in beluga (Huso huso)</title></titleInfo><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Keyvanshokooh</namePart><affiliation>Department of Fisheries, College of Natural Resources and Marine Sciences, Tarbiat Modares University, Mazandaran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Pourkazemi</namePart><affiliation>International Sturgeon Research Institute, Rasht, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. R.</namePart><namePart type="family">Kalbassi</namePart><affiliation>Department of Fisheries, College of Natural Resources and Marine Sciences, Tarbiat Modares University, Mazandaran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2007-02</dateIssued><edition>Received: February 20, 2006 Accepted: March 27, 2006</edition><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Because the sex of sturgeon cannot be determined externally until the pre‐spawning phase, they are currently sexed by means of minor surgery. Although the survival rate is nearly 100%, this surgery is invasive. One effective solution is to use DNA markers to diagnose the sex. In this study we employed bulked segregant analysis methodology to screen for genetic markers associated with the sex of the beluga (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 310 randomly amplified polymorphic DNA primers was used to screen the bulks, resulting in 4146 bands that were present in both sexes. Our results suggest that either the sex‐specific DNA in sturgeon may be comprised of sequences not complementary to 310 random decamer primers or that sex chromosomes are weakly differentiated in the sturgeon genome.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>1</start><end>2</end><total>2</total></extent></part></relatedItem><identifier type="istex">E83E633A436CA360E8E08ED338B1674FF81750A7</identifier><identifier type="DOI">10.1111/j.1439-0426.2006.00798.x</identifier><identifier type="ArticleID">JAI798</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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