Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus
Identifieur interne : 000B21 ( Main/Merge ); précédent : 000B20; suivant : 000B22Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus
Auteurs : G. Z. Zhou [République populaire de Chine] ; L. Gui [République populaire de Chine] ; Z. Q. Li [République populaire de Chine] ; X. P. Yuan [République populaire de Chine] ; Q. Y. Zhang [République populaire de Chine]Source :
- Journal of Fish Biology [ 0022-1112 ] ; 2008-12.
English descriptors
- KwdEn :
Abstract
Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a ‘living fossil’ on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail‐fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2n= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron‐microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV‐infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV‐infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.
Url:
DOI: 10.1111/j.1095-8649.2008.02076.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000734
- to stream Istex, to step Curation: 000732
- to stream Istex, to step Checkpoint: 000521
Links to Exploration step
ISTEX:6A4A097C2B372634091F303DC586658E8FE7C9E1Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus</title><author><name sortKey="Zhou, G Z" sort="Zhou, G Z" uniqKey="Zhou G" first="G. Z." last="Zhou">G. Z. Zhou</name></author><author><name sortKey="Gui, L" sort="Gui, L" uniqKey="Gui L" first="L." last="Gui">L. Gui</name></author><author><name sortKey="Li, Z Q" sort="Li, Z Q" uniqKey="Li Z" first="Z. Q." last="Li">Z. Q. Li</name></author><author><name sortKey="Yuan, X P" sort="Yuan, X P" uniqKey="Yuan X" first="X. P." last="Yuan">X. P. Yuan</name></author><author><name sortKey="Zhang, Q Y" sort="Zhang, Q Y" uniqKey="Zhang Q" first="Q. Y." last="Zhang">Q. Y. Zhang</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6A4A097C2B372634091F303DC586658E8FE7C9E1</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1111/j.1095-8649.2008.02076.x</idno><idno type="url">https://api.istex.fr/document/6A4A097C2B372634091F303DC586658E8FE7C9E1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000734</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000734</idno><idno type="wicri:Area/Istex/Curation">000732</idno><idno type="wicri:Area/Istex/Checkpoint">000521</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000521</idno><idno type="wicri:doubleKey">0022-1112:2008:Zhou G:establishment:of:a</idno><idno type="wicri:Area/Main/Merge">000B21</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus</title><author><name sortKey="Zhou, G Z" sort="Zhou, G Z" uniqKey="Zhou G" first="G. Z." last="Zhou">G. Z. Zhou</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072</wicri:regionArea><wicri:noRegion>Wuhan 430072</wicri:noRegion></affiliation></author><author><name sortKey="Gui, L" sort="Gui, L" uniqKey="Gui L" first="L." last="Gui">L. Gui</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072</wicri:regionArea><wicri:noRegion>Wuhan 430072</wicri:noRegion></affiliation></author><author><name sortKey="Li, Z Q" sort="Li, Z Q" uniqKey="Li Z" first="Z. Q." last="Li">Z. Q. Li</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072</wicri:regionArea><wicri:noRegion>Wuhan 430072</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, X P" sort="Yuan, X P" uniqKey="Yuan X" first="X. P." last="Yuan">X. P. Yuan</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072</wicri:regionArea><wicri:noRegion>Wuhan 430072</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Q Y" sort="Zhang, Q Y" uniqKey="Zhang Q" first="Q. Y." last="Zhang">Q. Y. Zhang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072</wicri:regionArea><wicri:noRegion>Wuhan 430072</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Fish Biology</title><idno type="ISSN">0022-1112</idno><idno type="eISSN">1095-8649</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2008-12">2008-12</date><biblScope unit="volume">73</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="2058">2058</biblScope><biblScope unit="page" to="2067">2067</biblScope></imprint><idno type="ISSN">0022-1112</idno></series><idno type="istex">6A4A097C2B372634091F303DC586658E8FE7C9E1</idno><idno type="DOI">10.1111/j.1095-8649.2008.02076.x</idno><idno type="ArticleID">JFB2076</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1112</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>chromosome</term><term>cytopathic effect</term><term>electron microscope</term><term>freshwater fish</term><term>immunofluorescence</term><term>karyotype</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a ‘living fossil’ on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail‐fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2n= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron‐microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV‐infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV‐infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B21 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000B21 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:6A4A097C2B372634091F303DC586658E8FE7C9E1
|texte= Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus
}}
| This area was generated with Dilib version V0.6.27. |  |