Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus
Identifieur interne : 000734 ( Istex/Corpus ); précédent : 000733; suivant : 000735Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus
Auteurs : G. Z. Zhou ; L. Gui ; Z. Q. Li ; X. P. Yuan ; Q. Y. ZhangSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2008-12.
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Abstract
Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a ‘living fossil’ on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail‐fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2n= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron‐microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV‐infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV‐infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.
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DOI: 10.1111/j.1095-8649.2008.02076.x
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ISTEX:6A4A097C2B372634091F303DC586658E8FE7C9E1Le document en format XML
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A cell line was established from Chinese sturgeon tail‐fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2n= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron‐microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV‐infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV‐infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. 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A cell line was established from Chinese sturgeon tail‐fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2n= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron‐microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV‐infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV‐infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>chromosome</term></item><item><term>cytopathic effect</term></item><item><term>electron microscope</term></item><item><term>freshwater fish</term></item><item><term>immunofluorescence</term></item><item><term>karyotype</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/6A4A097C2B372634091F303DC586658E8FE7C9E1/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1095-8649</doi><issn type="print">0022-1112</issn><issn type="electronic">1095-8649</issn><idGroup><id type="product" value="JFB"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF FISH BIOLOGY">Journal of Fish Biology</title></titleGroup></publicationMeta><publicationMeta level="part" position="12008"><doi origin="wiley">10.1111/jfb.2008.73.issue-8</doi><numberingGroup><numbering type="journalVolume" number="73">73</numbering><numbering type="journalIssue" number="8">8</numbering></numberingGroup><coverDate startDate="2008-12">December 2008</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="14" status="forIssue"><doi origin="wiley">10.1111/j.1095-8649.2008.02076.x</doi><idGroup><id type="unit" value="JFB2076"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="tocHeading1">REGULAR PAPERS</title></titleGroup><copyright>© 2008 The Authors Journal compilation © 2008 The Fisheries Society of the British Isles</copyright><eventGroup><event type="firstOnline" date="2008-11-28"></event><event type="publishedOnlineFinalForm" date="2008-11-28"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-02-28"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-30"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="2058">2058</numbering><numbering type="pageLast" number="2067">2067</numbering></numberingGroup><correspondenceTo> *Tel.: +86 27 68780792; fax: +86 27 68780123; email: <email>zhangqy@ihb.ac.cn</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JFB.JFB2076.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory><i>(Received 27 February 2008, Accepted 21 August 2008</i> <i>)</i></unparsedEditorialHistory><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="22"></count><count type="wordTotal" number="0"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Establishment of a Chinese sturgeon <i>Acipenser sinensis</i> tail‐fin cell line and its susceptibility to frog iridovirus</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>G. Z.</givenNames><familyName>Zhou</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>L.</givenNames><familyName>Gui</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Z. Q.</givenNames><familyName>Li</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>X. P.</givenNames><familyName>Yuan</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Q. Y.</givenNames><familyName>Zhang</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CN"><unparsedAffiliation><i>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</i></unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">chromosome</keyword><keyword xml:id="k2">cytopathic effect</keyword><keyword xml:id="k3">electron microscope</keyword><keyword xml:id="k4">freshwater fish</keyword><keyword xml:id="k5">immunofluorescence</keyword><keyword xml:id="k6">karyotype</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Chinese sturgeon <i>Acipenser sinensis</i>, a cartilaginous ganoid, is a ‘living fossil’ on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail‐fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2<i>n</i>= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [<i>Rana grylio</i> virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron‐microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV‐infected cells. Molecular analysis further suggested that <i>c.</i> 550 bp DNA fragment could be cloned from the RGV‐infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Establishment of a Chinese sturgeon Acipenser sinensis tail‐fin cell line and its susceptibility to frog iridovirus</title></titleInfo><name type="personal"><namePart type="given">G. Z.</namePart><namePart type="family">Zhou</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Gui</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z. Q.</namePart><namePart type="family">Li</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X. P.</namePart><namePart type="family">Yuan</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Q. Y.</namePart><namePart type="family">Zhang</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><affiliation>E-mail: zhangqy@ihb.ac.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2008-12</dateIssued><edition>(Received 27 February 2008, Accepted 21 August 2008 )</edition><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="references">22</extent></physicalDescription><abstract lang="en">Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a ‘living fossil’ on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail‐fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco’s modified Eagle’s medium at 25° C. Karyotypic analysis revealed a normal diploid karyotype with 2n= 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron‐microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV‐infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV‐infected CSTF cells’ DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies.</abstract><subject lang="en"><genre>keywords</genre><topic>chromosome</topic><topic>cytopathic effect</topic><topic>electron microscope</topic><topic>freshwater fish</topic><topic>immunofluorescence</topic><topic>karyotype</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>73</number></detail><detail type="issue"><caption>no.</caption><number>8</number></detail><extent unit="pages"><start>2058</start><end>2067</end><total>10</total></extent></part></relatedItem><identifier type="istex">6A4A097C2B372634091F303DC586658E8FE7C9E1</identifier><identifier type="DOI">10.1111/j.1095-8649.2008.02076.x</identifier><identifier type="ArticleID">JFB2076</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2008 The Authors Journal compilation © 2008 The Fisheries Society of the British Isles</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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