Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney.
Identifieur interne : 000A56 ( Main/Merge ); précédent : 000A55; suivant : 000A57Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney.
Auteurs : P. Ciba [Allemagne] ; S. Schicktanz ; E. Anders ; E. Siegl ; A. Stielow ; E. Klink ; C. KruseSource :
- Fish physiology and biochemistry [ 1573-5168 ] ; 2008.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Antibodies, Monoclonal, Keratins, Nerve Tissue Proteins, RNA-Binding Proteins.
- cytology : Adipocytes, Kidney.
- physiology : Fishes.
- Animals, Cells, Cultured, Immunohistochemistry, Time Factors.
Abstract
In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.
DOI: 10.1007/s10695-007-9196-8
PubMed: 18958594
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Anders</name></author><author><name sortKey="Siegl, E" sort="Siegl, E" uniqKey="Siegl E" first="E" last="Siegl">E. Siegl</name></author><author><name sortKey="Stielow, A" sort="Stielow, A" uniqKey="Stielow A" first="A" last="Stielow">A. Stielow</name></author><author><name sortKey="Klink, E" sort="Klink, E" uniqKey="Klink E" first="E" last="Klink">E. Klink</name></author><author><name sortKey="Kruse, C" sort="Kruse, C" uniqKey="Kruse C" first="C" last="Kruse">C. 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Ciba</name><affiliation wicri:level="1"><nlm:affiliation>Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Luebeck, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Luebeck</wicri:regionArea><wicri:noRegion>Luebeck</wicri:noRegion><wicri:noRegion>Luebeck</wicri:noRegion><wicri:noRegion>Luebeck</wicri:noRegion></affiliation></author><author><name sortKey="Schicktanz, S" sort="Schicktanz, S" uniqKey="Schicktanz S" first="S" last="Schicktanz">S. Schicktanz</name></author><author><name sortKey="Anders, E" sort="Anders, E" uniqKey="Anders E" first="E" last="Anders">E. Anders</name></author><author><name sortKey="Siegl, E" sort="Siegl, E" uniqKey="Siegl E" first="E" last="Siegl">E. Siegl</name></author><author><name sortKey="Stielow, A" sort="Stielow, A" uniqKey="Stielow A" first="A" last="Stielow">A. Stielow</name></author><author><name sortKey="Klink, E" sort="Klink, E" uniqKey="Klink E" first="E" last="Klink">E. Klink</name></author><author><name sortKey="Kruse, C" sort="Kruse, C" uniqKey="Kruse C" first="C" last="Kruse">C. Kruse</name></author></analytic><series><title level="j">Fish physiology and biochemistry</title><idno type="eISSN">1573-5168</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adipocytes (cytology)</term><term>Animals</term><term>Antibodies, Monoclonal (metabolism)</term><term>Cells, Cultured</term><term>Fishes (physiology)</term><term>Immunohistochemistry</term><term>Keratins (metabolism)</term><term>Kidney (cytology)</term><term>Nerve Tissue Proteins (metabolism)</term><term>RNA-Binding Proteins (metabolism)</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Keratins</term><term>Nerve Tissue Proteins</term><term>RNA-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Adipocytes</term><term>Kidney</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Immunohistochemistry</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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