Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney.
Identifieur interne : 000450 ( PubMed/Corpus ); précédent : 000449; suivant : 000451Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney.
Auteurs : P. Ciba ; S. Schicktanz ; E. Anders ; E. Siegl ; A. Stielow ; E. Klink ; C. KruseSource :
- Fish physiology and biochemistry [ 1573-5168 ] ; 2008.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Antibodies, Monoclonal, Keratins, Nerve Tissue Proteins, RNA-Binding Proteins.
- cytology : Adipocytes, Kidney.
- physiology : Fishes.
- Animals, Cells, Cultured, Immunohistochemistry, Time Factors.
Abstract
In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.
DOI: 10.1007/s10695-007-9196-8
PubMed: 18958594
Links to Exploration step
pubmed:18958594Le document en format XML
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<author><name sortKey="Ciba, P" sort="Ciba, P" uniqKey="Ciba P" first="P" last="Ciba">P. Ciba</name>
<affiliation><nlm:affiliation>Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Luebeck, Germany.</nlm:affiliation>
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<author><name sortKey="Schicktanz, S" sort="Schicktanz, S" uniqKey="Schicktanz S" first="S" last="Schicktanz">S. Schicktanz</name>
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<author><name sortKey="Anders, E" sort="Anders, E" uniqKey="Anders E" first="E" last="Anders">E. Anders</name>
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<author><name sortKey="Siegl, E" sort="Siegl, E" uniqKey="Siegl E" first="E" last="Siegl">E. Siegl</name>
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<author><name sortKey="Stielow, A" sort="Stielow, A" uniqKey="Stielow A" first="A" last="Stielow">A. Stielow</name>
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<author><name sortKey="Klink, E" sort="Klink, E" uniqKey="Klink E" first="E" last="Klink">E. Klink</name>
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<author><name sortKey="Kruse, C" sort="Kruse, C" uniqKey="Kruse C" first="C" last="Kruse">C. Kruse</name>
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<term>Fishes (physiology)</term>
<term>Immunohistochemistry</term>
<term>Keratins (metabolism)</term>
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<term>Nerve Tissue Proteins (metabolism)</term>
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<term>Nerve Tissue Proteins</term>
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<front><div type="abstract" xml:lang="en">In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.</div>
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<Abstract><AbstractText>In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.</AbstractText>
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